Overexpression of lncRNA SLC26A4‐AS1 inhibits papillary thyroid carcinoma progression through recruiting ETS1 to promote ITPR1‐mediated autophagy

Abstract

Papillary thyroid carcinoma (PTC), accounting for approximately 85% cases of thyroid cancer, is a common endocrine tumour with a relatively low mortality but an alarmingly high rate of recurrence or persistence. Long non‐coding RNAs (lncRNAs) is emerging as a critical player modulating diverse cellular mechanisms correlated with the progression of various cancers, including PTC. Herein, we aimed to investigate the role of lncRNA SLC26A4‐AS1 in regulating autophagy and tumour growth during PTC progression. Initially, ITPR1 was identified by bioinformatics analysis as a differentially expressed gene. Then, Western blot and RT‐qPCR were conducted to determine the expression of ITPR1 and SLC26A4‐AS1 in PTC tissues and cells, both of which were found to be poorly expressed in PTC tissues and cells. Then, we constructed ITPR1‐overexpressing cells and revealed that ITPR1 overexpression could trigger the autophagy of PTC cells. Further, we performed a series of gain‐ and loss‐of function experiments. The results suggested that silencing of SLC26A4‐AS1 led to declined ITPR1 level, up‐regulation of ETS1 promoted ITPR1 expression, and either ETS1 knockdown or autophagy inhibitor Bafilomycin A1 could mitigate the promoting effects of SLC26A4‐AS1 overexpression on PTC cell autophagy. In vivo experiments also revealed that SLC26A4‐AS1 overexpression suppressed PTC tumour growth. In conclusion, our study elucidated that SLC26A4‐AS1 overexpression promoted ITPR1 expression through recruiting ETS1 and thereby promotes autophagy, alleviating PTC progression. These finding provides insight into novel target therapy for the clinical treatment of PTC.

Identification and Validation of Autophagy-Related Genes in Diabetic Retinopathy

“…P-value rankings when>3 terms were enriched for a given category. ischemic stroke and osteoporosis (39)(40)(41)(42). However, the role of these genes in modulating autophagy in DR has not been fully explored.…”

A seven-autophagy-related gene signature for predicting the prognosis of differentiated thyroid carcinoma

“…ITPR1-mediated induction of autophagy is thought to promote renal cell carcinoma by suppressing NK cells [ 53 ]. In papillary thyroid carcinoma, expression of ITPR1 was promoted via effect of lncRNA SLC26A4-AS1 mediated ETS1 recruitment, suppressing tumour growth by enhancing autophagy [ 54 ]. However, regression analysis revealed high ITPR1 expression is associated with poor THCA prognosis.…”

Overexpression of lncRNA SLC26A4‐AS1 inhibits papillary thyroid carcinoma progression through recruiting ETS1 to promote ITPR1‐mediated autophagy

“…In Xuefen Liu et al 1 the immunofluorescence diagram pcDNA30.1 in Figure 2C, the WB experiments in Figure 1E,F are incorrect. The correct figure is shown below.…”