Overexpression of lncRNA-Gm2044 in spermatogonia impairs spermatogenesis in partial seminiferous tubules

Hu, Ke; Gao, Yuanyuan; Xu, Yifan; He, Chunyan; Wang, Kaixian; Li, Leina; Liao, Yaping; Liu, Xiaohua; Liang, Meng

doi:10.1016/j.psj.2022.101930

Cited by 4 publications

(3 citation statements)

References 34 publications

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“…Liu et al (2021) also found that knockdown testis-specific lncRNA Rbkdn in mice disturbs meiosis, reduces testicular volume, and increases apoptosis of spermatocytes. Hu et al (2022) found that overexpression of lncRNA-Gm2044 in transgenic mouse spermatogonia impairs spermatogenesis. In this study, it was found that LncRNA-IMS is involved in the regulation of gga-miR-31-5p-Stra8 signal chain.…”

Section: Discussionmentioning

confidence: 99%

Jun‐mediated lncRNA‐IMS promotes the meiosis of chicken spermatogonial stem cells via gga‐miR‐31‐5p/stra8

Wang

Zhang

Kong

et al. 2023

Molecular Reproduction Devel

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Meiosis, a key step in spermatogenesis, is affected by many factors. Current studies have shown that long noncoding RNAs (lncRNAs) are potential factors regulating meiosis, and their regulatory mechanisms have received much attention. However, little research has been done on its regulatory mechanism in the spermatogenesis of roosters. Here, we found that lncRNA involved in meiosis and spermatogenesis (lncRNA-IMS) was involved in the regulation of Stra8 by gga-miR-31-5p and hindered the inhibition of Stra8 by gga-miR-31-5p. The acquisition and loss of function experiments demonstrated that lncRNA-IMS was involved in meiosis and spermatogenesis. In addition, we predicted and determined the core promoter region of lncRNA-IMS. Prediction of transcription factors, deletion/ overexpression of binding sites, knockdown/overexpression of Jun, and dualluciferase reporter analysis confirmed that Jun positively activated transcription of lncRNA-IMS. Our findings further enrich the TF-lncRNA-miRNA-mRNA regulatory network during male meiosis and provide new ideas for studying the molecular mechanism of meiosis and spermatogenesis in chicken spermatogonial stem cells.

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Section: Discussionmentioning

confidence: 99%

Jun‐mediated lncRNA‐IMS promotes the meiosis of chicken spermatogonial stem cells via gga‐miR‐31‐5p/stra8

Wang

Zhang

Kong

et al. 2023

Molecular Reproduction Devel

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show abstract

“…This study provided new insight into the mechanistic roles of lncRNA Gm2044/miR‐335‐3p in the regulation of spermatocyte function by transcription factor A‐MYB (Liang et al, 2020). Importantly, using the transgenic mouse model of lncRNA‐Gm2044, researchers found that the upregulation of lncRNA‐Gm2044 did not affect testicular morphology and fertility but partially impaired spermatogenesis in transgenic mice (Hu et al, 2022).…”

Section: The Roles Of Lncrnas In Male Reproductionmentioning

confidence: 99%

Long noncoding RNAs in human reproductive processes and diseases

Zhang,

Sun,

Chen

2024

Molecular Reproduction Devel

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Infertility has become a global disease burden. Although assisted reproductive technologies are widely used, the assisted reproduction birth rate is no more than 30% worldwide. Therefore, understanding the mechanisms of reproduction can provide new strategies to improve live birth rates and clinical outcomes of enhanced implantation. Long noncoding RNAs (lncRNAs) have been reported to exert regulatory roles in various biological processes and diseases in many species. In this review, we especially focus on the role of lncRNAs in human reproduction. We summarize the function and mechanisms of lncRNAs in processes vital to reproduction, such as spermatogenesis and maturation, sperm motility and morphology, follicle development and maturation, embryo development and implantation. Then, we highlight the importance and diverse potential of lncRNAs as good diagnostic molecular biomarkers and therapeutic targets for infertility treatment.

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“…The role of long non-coding RNAs (lncRNAs) like Gm2044 and GULP1-2:1 in the realm of reproductive health, particularly within the context of ovarian function, is an area of growing significance and scientific exploration [ 54 , 55 ]. LncRNA Gm2044 has garnered attention due to its substantial involvement in male reproductive processes, and it's noteworthy that this lncRNA is abundantly expressed in both testicular and ovarian tissues.…”

Section: Correlation Between Lncrna As Cerna and Ovarian Functionmentioning

confidence: 99%

The functional role of lncRNAs as ceRNAs in both ovarian processes and associated diseases

Usman,

Li,

et al. 2024

Non-coding RNA Research

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Overexpression of lncRNA-Gm2044 in spermatogonia impairs spermatogenesis in partial seminiferous tubules

Cited by 4 publications

References 34 publications

Jun‐mediated lncRNA‐IMS promotes the meiosis of chicken spermatogonial stem cells via gga‐miR‐31‐5p/stra8

Jun‐mediated lncRNA‐IMS promotes the meiosis of chicken spermatogonial stem cells via gga‐miR‐31‐5p/stra8

Long noncoding RNAs in human reproductive processes and diseases

The functional role of lncRNAs as ceRNAs in both ovarian processes and associated diseases

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