Overexpression of lipid transfer protein (LTP) genes enhances resistance to plant pathogens and LTP functions in long-distance systemic signaling in tobacco

Sarowar, Sujon; Kim, Young Jin; Kim, Ki Deok; Hwang, Byung Kook; Ok, Sung Han; Shin, Jeong Sheop

doi:10.1007/s00299-008-0653-3

Cited by 111 publications

(56 citation statements)

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“…Later, these lipid transfer molecules were shown to be able to transport a variety of hydrophobic molecules, such as fatty acids (Han et al, 2001), fatty acyl-CoA (Lerche et al, 1997), lysophosphatidylcholine (Charvolin et al, 1999), and phosphatidylglycerol (Sodano et al, 1997). LTPs in plants are thought to play key roles in many biological processes, such as cutin biosynthesis (Kim et al, 2008;Lee et al, 2009), defense response (Lee et al, 2009;Sarowar et al, 2009), plant signaling (Blein et al, 2002Maldonado et al, 2002), and seed maturation (Thoma et al, 1994). In the rice genome, 52 LTP genes have been annotated (Boutrot et al, 2008).…”

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confidence: 99%

OsC6, Encoding a Lipid Transfer Protein, Is Required for Postmeiotic Anther Development In Rice

et al. 2010

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Synthesis of lipidic components in anthers, including of the pollen exine, is essential for plant male reproductive development. Plant lipid transfer proteins (LTPs) are small, abundant lipid-binding proteins that have the ability to exchange lipids between membranes in vitro. However, their biological role in male reproductive development remains less understood. Here, we report the crucial role of OsC6 in regulating postmeiotic anther development in rice (Oryza sativa). Found in monocots, OsC6 belongs to a distinct clade from previously identified LTP1 and LTP2 family members found in both dicots and monocots. OsC6 expression is mainly detectable in tapetal cells and weakly in microspores from stage 9 to stage 11 of anther development. Immunological assays indicated that OsC6 is widely distributed in anther tissues such as the tapetal cytoplasm, the extracellular space between the tapetum and middle layer, and the anther locule and anther cuticle. Biochemical assays indicated that recombinant OsC6 has lipid binding activity. Moreover, plants in which OsC6 was silenced had defective development of orbicules (i.e. Ubisch bodies) and pollen exine and had reduced pollen fertility. Furthermore, additional evidence is provided that the expression of OsC6 is positively regulated by a basic helix-loop-helix transcription factor, Tapetum Degeneration Retardation (TDR). Extra granule-like structures were observed on the inner surface of the tdr tapetal layer when the expression of OsC6 was driven by the TDR promoter compared with the tdr mutant. These data suggest that OsC6 plays a crucial role in the development of lipidic orbicules and pollen exine during anther development in rice.

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confidence: 99%

OsC6, Encoding a Lipid Transfer Protein, Is Required for Postmeiotic Anther Development In Rice

et al. 2010

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“…The study of how intracellular pathogens manipulate lipid traffic and membrane biogenesis is an expanding research area (Sarowar et al, 2009;Mutsafi et al, 2013;Barajas et al, 2014b;Konan and Sanchez-Felipe, 2014;Nour and Modis, 2014;Paul et al, 2014). We show the importance of 3D imaging when combined with new methods for sensitive molecular mapping to better understand viral replication in cells.…”

Section: Discussionmentioning

confidence: 93%

Three dimensional imaging of the intracellular assembly of a functional viral RNA replicase complex

Castro

Fernández

Barajas

et al. 2016

Journal of Cell Science

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Positive-strand RNA viruses, which can be devastating pathogens in humans, animals and plants, replicate their genomes on intracellular membranes. Here, we describe the three-dimensional ultrastructural organization of a tombusvirus replicase in yeast, a valuable model for exploring virus-host interactions. We visualized the intracellular distribution of a viral replicase protein using metal-tagging transmission electron microscopy, a highly sensitive nanotechnology whose full potential remains to be developed. These three-dimensional images show how viral replicase molecules are organized when they are incorporated into the active domains of the intracellular replication compartment. Our approach provides a means to study protein activation mechanisms in cells and to identify targets for new antiviral compounds.

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“…Genetic approaches to identifying signals sent during virus infection include selective partial suppression of transcripts via virus-induced gene silencing (VIGS) (Wu et al 2008;Sarowar et al 2009;Cheng et al 2010;Chen et al 2009), complete knockout of selected genes by mutagenesis (Xia et al 2008;Lim et al 2010), overexpression of a plant (Vannini et al 2006;Quilis et al 2008) or virus (Geri et al 1999) transgene, and two-hybrid interaction studies (Piroux et al 2007;Carvalho et al 2008). …”

Section: Review Of Methodsmentioning

confidence: 97%

Virus Operation Control Centers

Melcher

2011

Biocommunication of Plants

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The nucleic acids of plant viruses are the control centers that coordinate all activities associated with virus survival and propagation within cells, in whole plants and between organisms. Within cells, the viruses use a diversity of signaling mechanisms to assure the orderly production at specific subcellular locations of viral mRNAs, viral proteins, viral genomic nucleic acids, and viral particles and the export of infectious entities to neighboring cells. Within cells, viruses also signal their presence to the host cell machinery, establishing the conditions of coexistence of virus and plant in successful infections. At the plant level, the control centers direct the movement of infectious entities from one cell to another, into the vascular system, and into tissues remote from the site of initial infection. At the same time, the control centers condition the plant to be hospitable to virus reproduction and survival. They also cause the plant to issue signals to potential vectors guiding them to the plant to acquire the virus and encouraging their departure to further plants, in effect spreading the virus among multiple plants. The signals used in these processes include small molecules (hormones and volatiles), macromolecules with binding sites for other molecules (some being enzymatic), macromolecular structure conformations, genomic organizations, and others. Often, different viruses accomplish the same activity in completely different ways, although some common strategies are employed.

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Overexpression of lipid transfer protein (LTP) genes enhances resistance to plant pathogens and LTP functions in long-distance systemic signaling in tobacco

Cited by 111 publications

References 34 publications

OsC6, Encoding a Lipid Transfer Protein, Is Required for Postmeiotic Anther Development In Rice

OsC6, Encoding a Lipid Transfer Protein, Is Required for Postmeiotic Anther Development In Rice

Three dimensional imaging of the intracellular assembly of a functional viral RNA replicase complex

Virus Operation Control Centers

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