Overexpression of JcWRKY2 confers increased resistance towards Macrophomina phaseolina in transgenic tobacco

Dabi, Mitali; Agarwal, Parinita; Agarwal, Pradeep K.

doi:10.1007/s13205-020-02490-0

Cited by 8 publications

(6 citation statements)

References 36 publications

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“…mali ( Zhang et al., 2017 ). Other WRKY TFs, such as WRKY11 ( Lee et al., 2018 ), WRKY13 ( Wang et al., 2019 ), WRKY2 ( Dabi et al., 2020 ), WRKY67 ( Vo et al., 2017 ), WRKY62, WRKY28, WRKY71, and WRKY76 ( Peng et al., 2010 ) were identified in this SR and acted by regulating PR-10 expression in biotic stress. Taken together, these data revealed the regulatory network that PR-10 is part of, involving phytohormones and TFs, to help guide the plant’s resistance to the stressor agent.…”

Section: Discussionmentioning

confidence: 88%

“…These processes include the SA and JA hormonal signaling pathways, activating the expression of PR-10, as well as the WRKY13 TF, which also acts by activating the transcription of this protein ( Figure 5 ) ( Wang et al., 2019 ). Other molecules were seen as active in the PR-10 signaling network, where they positively regulated PR-10 transcription, such as COL9 (Constans-Like9) ( Liu et al., 2016 ); CAD12 (cinnamyl alcohol dehydrogenase enzyme) ( Rong et al., 2016 ); UPL-BOI (E3 ubiquitin protein ligase) ( Khorramdelazad et al., 2018 ); and the TFs WRKY2 ( Dabi et al., 2020 ), WRKY67 ( Vo et al., 2017 ), WRKY26, WRKYN1 ( Zhang et al., 2017 ), PIMP2 ( Wei et al., 2017 ), ERF83 ( Tezuka et al., 2019 ), RIM1 ( Shan et al., 2016 ), GATA1 ( Liu et al., 2019 ), and PIE1 ( Zhu et al., 2014 ). Systematized data showed that in stress caused by fungi, PR-10, when induced, acted with RNase function ( Xie et al., 2010 ), degrading the RNA of the invading fungus and binding to cytokinin to mitigate the damage that the fungal stress caused ( Agarwal et al., 2016 ).…”

Section: Resultsmentioning

confidence: 99%

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Pathogenesis-related protein 10 in resistance to biotic stress: progress in elucidating functions, regulation and modes of action

et al. 2023

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IntroductionThe Family of pathogenesis-related proteins 10 (PR-10) is widely distributed in the plant kingdom. PR-10 are multifunctional proteins, constitutively expressed in all plant tissues, playing a role in growth and development or being induced in stress situations. Several studies have investigated the preponderant role of PR-10 in plant defense against biotic stresses; however, little is known about the mechanisms of action of these proteins. This is the first systematic review conducted to gather information on the subject and to reveal the possible mechanisms of action that PR-10 perform.MethodsTherefore, three databases were used for the article search: PubMed, Web of Science, and Scopus. To avoid bias, a protocol with inclusion and exclusion criteria was prepared. In total, 216 articles related to the proposed objective of this study were selected.ResultsThe participation of PR-10 was revealed in the plant’s defense against several stressor agents such as viruses, bacteria, fungi, oomycetes, nematodes and insects, and studies involving fungi and bacteria were predominant in the selected articles. Studies with combined techniques showed a compilation of relevant information about PR-10 in biotic stress that collaborate with the understanding of the mechanisms of action of these molecules. The up-regulation of PR-10 was predominant under different conditions of biotic stress, in addition to being more expressive in resistant varieties both at the transcriptional and translational level.DiscussionBiological models that have been proposed reveal an intrinsic network of molecular interactions involving the modes of action of PR-10. These include hormonal pathways, transcription factors, physical interactions with effector proteins or pattern recognition receptors and other molecules involved with the plant’s defense system.ConclusionThe molecular networks involving PR-10 reveal how the plant’s defense response is mediated, either to trigger susceptibility or, based on data systematized in this review, more frequently, to have plant resistance to the disease.

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Section: Discussionmentioning

confidence: 88%

Section: Resultsmentioning

confidence: 99%

Pathogenesis-related protein 10 in resistance to biotic stress: progress in elucidating functions, regulation and modes of action

et al. 2023

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“…Similarly, ERFs were reported to be induced by pathogens, ethylene, JA, and regulate the expression of downstream pathogenesisrelated genes [38,39]. It has been shown that overexpression of WRKY and ERF TFs resulted in increased resistance against fungal pathogens [40,41]. Similarly, it is well known that the expression of a few TF families can increase susceptibility to fungal pathogens, and one among them is the homeodomain-leucine zipper (HD-ZIP).…”

Section: Discussionmentioning

confidence: 99%

Comprehensive transcriptome analysis of different potato cultivars provides insight into early blight disease caused by Alternaria solani

et al. 2023

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Background Early blight, caused by the necrotrophic fungal pathogen Alternaria solani, is an economically important disease affecting the tuber yield worldwide. The disease is mainly controlled by chemical plant protection agents. However, over-using these chemicals can lead to the evolution of resistant A. solani strains and is environmentally hazardous. Identifying genetic disease resistance factors is crucial for the sustainable management of early blight but little effort has been diverted in this direction. Therefore, we carried out transcriptome sequencing of the A. solani interaction with different potato cultivars with varying levels of early blight resistance to identify key host genes and pathways in a cultivar-specific manner. Results In this study, we have captured transcriptomes from three different potato cultivars with varying susceptibility to A. solani, namely Magnum Bonum, Désirée, and Kuras, at 18 and 36 h post-infection. We identified many differentially expressed genes (DEGs) between these cultivars, and the number of DEGs increased with susceptibility and infection time. There were 649 transcripts commonly expressed between the potato cultivars and time points, of which 627 and 22 were up- and down-regulated, respectively. Interestingly, overall the up-regulated DEGs were twice in number as compared to down-regulated ones in all the potato cultivars and time points, except Kuras at 36 h post-inoculation. In general, transcription factor families WRKY, ERF, bHLH, MYB, and C2H2 were highly enriched DEGs, of which a significant number were up-regulated. The majority of the key transcripts involved in the jasmonic acid and ethylene biosynthesis pathways were highly up-regulated. Many transcripts involved in the mevalonate (MVA) pathway, isoprenyl-PP, and terpene biosynthesis were also up-regulated across the potato cultivars and time points. Compared to Magnum Bonum and Désirée, multiple components of the photosynthesis machinery, starch biosynthesis and degradation pathway were down-regulated in the most susceptible potato cultivar, Kuras. Conclusions Transcriptome sequencing identified many differentially expressed genes and pathways, thereby contributing to the improved understanding of the interaction between the potato host and A. solani. The transcription factors identified are attractive targets for genetic modification to improve potato resistance against early blight. The results provide important insights into the molecular events at the early stages of disease development, help to shorten the knowledge gap, and support potato breeding programs for improved early blight disease resistance.

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“…Similarly, ERFs were reported to be induced by pathogens, ethylene, JA, and regulate the expression of downstream pathogenesis-related genes [52,53]. It has been shown that overexpression of WRKY and ERF TFs resulted in increased resistance against fungal pathogens [54,55]. Similarly, it is well known that the expression of a few TF families can increase susceptibility to fungal pathogens, and one among them is the homeodomain-leucine zipper (HD-ZIP).…”

Section: Discussionmentioning

confidence: 99%

Comprehensive transcriptome analysis of different potato cultivars provides insight into early blight disease caused by Alternaria solani

Sivarajan

Abdelmeguid

Saripella

et al. 2022

Preprint

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Background Early blight disease in potatoes is one of the economically important diseases affecting the tuber yield and quality worldwide. It is caused by the necrotrophic fungal pathogen Alternaria solani. The disease is mainly controlled by chemical plant protection agents. However, the over-usage of these chemicals leads to the evolution of resistant A. solani strains and is environmentally hazardous. Therefore, identifying resistant potato cultivars or genetic disease resistance factors is the best strategy for the sustainable management of early blight. Results In this study, we have captured transcriptomes from three different potato cultivars with varying susceptibility to A. solani, Magnum Bonum, Désirée, and Kuras, at 18 and 36 hours post-infection. We identified many differentially expressed genes (DEGs) between these cultivars, and the number of DEGs increased with susceptibility and infection time. There were 649 transcripts commonly expressed between the potato cultivars and time points, of which 627 and 22 were up- and down-regulated, respectively. Interestingly, overall the up-regulated DEGs were twice in number as compared to down-regulated ones in all the potato cultivars and time points, except Kuras at 36 hours post-inoculation. In general, transcription factor families WRKY, ERF, bHLH, MYB, and C2H2 were highly enriched DEGs, of which a significant number were up-regulated. The majority of the key transcripts involved in the jasmonic acid and ethylene biosynthesis pathways were highly up-regulated. Many transcripts involved in the mevalonate (MVA) pathway, isoprenyl-PP, and terpene biosynthesis were also up-regulated across the potato cultivars and time points. Compared to Magnum Bonum and Désirée, multiple components of the photosynthesis machinery, starch biosynthesis and degradation pathway were down-regulated in the most susceptible potato cultivar, Kuras. Conclusions This study provides important insights into the molecular events occurring in early disease development and helps to shorten the knowledge gap and supports potato breeding programs for improved early blight disease resistance.

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Overexpression of JcWRKY2 confers increased resistance towards Macrophomina phaseolina in transgenic tobacco

Cited by 8 publications

References 36 publications

Pathogenesis-related protein 10 in resistance to biotic stress: progress in elucidating functions, regulation and modes of action

Pathogenesis-related protein 10 in resistance to biotic stress: progress in elucidating functions, regulation and modes of action

Comprehensive transcriptome analysis of different potato cultivars provides insight into early blight disease caused by Alternaria solani

Comprehensive transcriptome analysis of different potato cultivars provides insight into early blight disease caused by Alternaria solani

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