Overexpression of IL-1ra gene up-regulates interleukin-1β converting enzyme (ICE) gene expression: Possible mechanism underlying IL-1β-resistance of cancer cells

Sumitomo, Makoto; Tachibana, Masaaki; Murai, Masaru; Hayakawa, Masamichi; Nakamura, Haruo; Takayanagi, Atsushi; Shimizu, Nobuyoshi

doi:10.1038/sj.bjc.6690688

Cited by 8 publications

(4 citation statements)

References 41 publications

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“…Previous studies have used one or both of these cytotoxicity assays and/or the differential dye uptake assay in combination with other assay methods (Edwards and Tolkovsky, 1994;Hardwick et al;Hughes et al, 1996;Burger et al, 1999;Lizard et al, 1999;McGuinness et al, 1999;Sumitomo et al, 1999;DeMeester et al, 1997). However, none have used this specific combination of assays in evaluating apoptosis in breast cancer cell lines.…”

Section: Discussionmentioning

confidence: 99%

Staurosporine-induced apoptosis and hydrogen peroxide-induced necrosis in two human breast cell lines

2003

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The use of apoptosis-inducing agents in the treatment of malignant cancer is increasingly being considered as a therapeutic approach. In this study, the induction of apoptosis and necrosis was examined in terms of temporal dose responses, comparing a malignant and nonmalignant breast cell line. Staurosporine (SSP)-induced apoptosis and H 2 O 2 -induced necrosis were evaluated by two cytotoxicity assays, neutral red (NR) and methyl-thiazolyl tertrazolium (MTT), in comparison with a differential dye uptake assay, using Hoechst33342/propidium iodide (Hoechst/PI). Confirmatory morphological assessment was also performed by routine resin histology and transmission electron microscopy. Cell viability was assessed over a 0.5 -48 h time course. In nonmalignant HBL-100 cells, 50 nM SSP induced 100% apoptosis after a 48 h exposure, while the same exposure to SSP caused only 4% apoptosis in metastatic T47D cells. Although complete apoptosis of both cell lines was induced by 50 mM SSP, this effect was delayed in T47D (24 h) compared with HBL-100 (4 h). Results also showed that neither MTT or NR can distinguish between the modes of cell death, nor detect the early onset of apoptosis revealed by Hoechst/PI.

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Section: Discussionmentioning

confidence: 99%

Staurosporine-induced apoptosis and hydrogen peroxide-induced necrosis in two human breast cell lines

2003

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“…The male host spleen cDNA, which was transplanted irradiated spleen, amplified the bcl-2 and LICE genes in the RT-PCR, while the female host spleen cDNA, which was transplanted irradiated spleen, amplified the bcl-2 gene and small amounts of the bcl-xS gene in the RT-PCR. Interleukin 1b converting enzyme (ICE)/caspase-1 is the enzyme responsible for cleaving pre IL-1b [13]. Soluble IL-1 receptor antagonist (sIL-1ra) enhances ICE gene expression.…”

Section: Discussionmentioning

confidence: 99%

A Type of Rat Hepatosplenic Gamma-delta (γδ) T-cell Lymphoma Developed after Injections with Hepatocyte Growth Factor (HGF) Oligonucleotides, which was Rejected by Surviving Syngeneic Spleen Graft

Nakatsuji

2003

Leukemia & Lymphoma

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The transduction of exogenous hepatocyte growth factor (HGF) genes to spleen T lymphocytes and the immune effects of syngeneic spleen graft on spleen lymphoma cells were studied in LEW/Sea rats. Three different systems were designed. (1) Six female rats and six male rats received irradiated spleen graft and were followed for 7 months. (2) Five female rats and six male rats received intra-peritoneal (i.p.) injections of the Lewis red cells incorporated 20-bp HGF genes and anti-interleukin-6 (IL-6) antibody (Ab) with spleen graft and were followed for 5.5 months. (3) Four females and five males received i.p. injections of the Lewis red cells incorporated 20-bp HGF genes and anti-IL-6 Ab without spleen graft and were followed for 5.5 months. Hemato-pathological analyses, flow cytometer analyses of gamma-delta (gammadelta) T-cell receptor (TCR)-positive lymphocytes and reverse-transcription-polymerase chain reaction (RT-PCR) of TCRgamma gene, TCRValpha3 gene and apoptotic genes were performed. Results showed that one of the six females received irradiated spleen graft developed nodal gamma-delta (gammadelta) T-cell pre-lymphoma with 100% of gammadelta TCR+ lymphocytes in the mesenteric lymph nodes. One female injected with the HGF genes and anti-IL-6 Ab and grafted spleen died of advanced hepatosplenic gammadelta T-cell lymphoma at 3.5 month observation. All the five males injected with the HGF genes and anti-IL-6 Ab without spleen graft developed early hepatosplenic gammadelta T-cell lymphoma at 5.5 month observation. In two rats with spleen graft and the two rats without spleen graft, which were injected with the HGF genes and anti-IL-6 Ab, the lymphoma was suspected uncertainly with activated TCRValpha3 mRNA. The i.p. injections of HGF genes, which were incorporated in the red blood cells, triggered hapatosplenic gammadelta T-cell lymphoma. Surviving spleen graft rejected the lymphoma cells, but not in rats rejected the graft. Spleen graft was a good organ transplantation to expect graft versus lymphoma effects.

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“…It is also possible that intracellular forms of IL-1Ra are more affected. A recent report related the overexpression of the IL-1Ra gene to the up-regulation of IL-1␤-converting enzyme expression (17). This could be related to altered processing and increased production of the important proinflammatory cytokines IL-1 and IL-18.…”

Section: Discussionmentioning

confidence: 99%

Higher frequency of allele 2 of the interleukin-1 receptor antagonist gene in patients with juvenile idiopathic arthritis

Vencovský¹,

Jarošová²,

Růžicková³

et al. 2001

Arthritis & Rheumatism

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Overexpression of IL-1ra gene up-regulates interleukin-1β converting enzyme (ICE) gene expression: Possible mechanism underlying IL-1β-resistance of cancer cells

Cited by 8 publications

References 41 publications

Staurosporine-induced apoptosis and hydrogen peroxide-induced necrosis in two human breast cell lines

Staurosporine-induced apoptosis and hydrogen peroxide-induced necrosis in two human breast cell lines

A Type of Rat Hepatosplenic Gamma-delta (γδ) T-cell Lymphoma Developed after Injections with Hepatocyte Growth Factor (HGF) Oligonucleotides, which was Rejected by Surviving Syngeneic Spleen Graft

Higher frequency of allele 2 of the interleukin-1 receptor antagonist gene in patients with juvenile idiopathic arthritis

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