Overexpression of ICE1, a Regulator of Cold-Induced Transcriptome, Confers Cold Tolerance to Transgenic Zoysia japonica

Zuo, Zhi-Fang; Kang, Hong‐Gyu; Park, Mi-Young; Jeong, Hana; Sun, Hui; Yang, Doo-Yeong; Lee, Yong‐Eok; Song, Pill‐Soon; Lee, Hyo-Yeon

doi:10.1007/s12374-018-0330-1

Cited by 23 publications

(6 citation statements)

References 57 publications

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“…ICE1 improved freezing tolerance in transgenic Arabidopsis thaliana [19]. The overexpression of ICE1 was reported to confer cold stress tolerance in transgenic zoysia grass [20]. Taken together our results reveal that the special activity of ICE1 and CBF1 genes provided cold stress defense in alfalfa seedlings.…”

Section: Molecular Insights Of Cold Stress Tolerance In Alfalfamentioning

confidence: 56%

“…Another key candidate is ICE1 (Inducer of CBF expression 1) which binds to MYC recognition cis-elements (CANNTG) in the promoter of CBF3/DREB and controls the transcription of CBF during CS adaptation in Arabidopsis [18]. Overexpression of ICE1 enhances freezing tolerance in transgenic Arabidopsis [19], Zoysia grass [20], cold-drought-salt tolerance in transgenic tobacco and rice [21,22]. However, it is still unclear how the combined roles of ICE1 and COR in cold stress tolerance in Medicago species.…”

Section: Introductionmentioning

confidence: 99%

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Mechanistic Basis of Silicon Mediated Cold Stress Tolerance in Alfalfa (Medicago sativa L.)

Rahman,

Song,

Hasan

et al. 2023

Silicon

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Cold stress (CS) impact on crops is one of the critical constraints for sustainable and smart agricultural production. CS adversely affects plants leading to growth retardation, necrosis, chlorosis, and significant yield loss. The objective of this study was to explore the mechanistic basis of silicon (Si) in enhancing CS tolerance in alfalfa plants. The fluorescence staining indicated that Si-reduced the intensity of CS-induced superoxide radical (O2•–) and hydrogen peroxide (H2O2) generation in plants that improved plant photosynthesis, cellular integrity, and alfalfa biomass production under CS. The exogenous supplementation of Si significantly restored the endogenous Si status accompanied by the upregulation of NIP (nodulin 26-like intrinsic protein) genes NIP2, NIP5;1, and NIP6;1 in alfalfa. The elemental concentration analysis revealed that exogenous silicon (E-Si) triggers the increase of calcium (Ca), magnesium (Mg), and sulfur (S) in plants subjected to Si-supplementation compared to the plants cultivated without Si under CS. The application of Si significantly increased the activity of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR). Furthermore, Si significantly enhanced the expression of CS-responsive candidate genes including ICE1, CBF1/DREB1C, CBF2/DREB1B, CBF3/DREB1A, COR15A, COR47, and KIN1 in alfalfa. These findings together provide mechanistic insights into Si-involving CS tolerance in alfalfa. This eco-friendly SC management strategy using Si treatment can be useful to plant breeders and farmers for developing CS-resilient smart alfalfa production through breeding program.

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Section: Molecular Insights Of Cold Stress Tolerance In Alfalfamentioning

confidence: 56%

Section: Introductionmentioning

confidence: 99%

Mechanistic Basis of Silicon Mediated Cold Stress Tolerance in Alfalfa (Medicago sativa L.)

Rahman,

Song,

Hasan

et al. 2023

Silicon

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“…Anthocyanins may scavenge ROS as an efficient antioxidant by neutralizing radicals with their hydroxyl groups to preserve normal cellular redox homeostasis; hence, plants often generate anthocyanins to reduce oxidative damage caused by low-temperature stress [ 20 , 21 , 22 , 23 , 24 , 25 ]. Low-temperature responsive networks in zoysiagrass have been investigated through physiological, biochemical, molecular, genetic and omic approaches [ 7 , 15 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 ]. However, no studies have attempted to associate anthocyanin accumulation with cold tolerance in zoysiagrass.…”

Section: Introductionmentioning

confidence: 99%

A Survey of Enhanced Cold Tolerance and Low-Temperature-Induced Anthocyanin Accumulation in a Novel Zoysia japonica Biotype

Jin

Jiang

Yang

et al. 2022

Plants

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Zoysia japonica is a warm-season turfgrass that is extensively used in landscaping, sports fields, and golf courses worldwide. Uncovering the low-temperature response mechanism of Z. japonica can help to accelerate the development of new cold-tolerant cultivars, which could be used to prolong the ornamental and usage duration of turf. A novel Z. japonica biotype, YueNong-9 (YN-9), was collected from northeastern China for this study. Phenotypic measurements, cold-tolerance investigation, and whole-transcriptome surveys were performed on YN-9 and LanYin-3 (LY-3), the most popular Z. japonica cultivar in Southern China. The results indicated the following: YN-9 has longer second and third leaves than LY-3; when exposed to the natural low temperature during winter in Guangzhou, YN-9 accumulated 4.74 times more anthocyanin than LY-3; after cold acclimation and freezing treatment, 83.25 ± 9.55% of YN-9 survived while all LY-3 leaves died, and the dark green color index (DGCI) value of YN-9 was 1.78 times that of LY-3; in YN-9, there was a unique up-regulation of Phenylalanine ammonia-lyase (PAL), Homeobox-leucine Zipper IV (HD-ZIP), and ATP-Binding Cassette transporter B8 (ABCB8) expressions, as well as a unique down-regulation of zinc-regulated transporters and iron-regulated transporter-like proteins (ZIPs) expression, which may promote anthocyanin biosynthesis, transport, and accumulation. In conclusion, YN-9 exhibited enhanced cold tolerance and is thus an excellent candidate for breeding cold-tolerant Z. japonica variety, and its unique low-temperature-induced anthocyanin accumulation and gene responses provide ideas and candidate genes for the study of low-temperature tolerance mechanisms and genetic engineering breeding.

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“… 1 The CA treatment modulates morphological, anatomical, physiological and biochemical properties of plants, significantly enhancing their stress resistance. 2 Plants acclimate to cold stress by accumulating osmotic regulators, such as soluble sugar (SS), 3 proline (Pro), 4 soluble protein (SP), and polyamines (PAs). 5 Plants exhibit enhanced activities of antioxidant enzymes, such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), glutathione peroxidase (GPX), and ascorbic acid peroxidase (APX) and eliminate excess reactive oxygen species (ROS) to alleviate the oxidative damage in plant cells under cold stress 6–9 Plants can also resist cold injury by increasing the proportion of unsaturated fatty acids (oleic acid, linoleic acid, and linolenic acid), 10 , 11 CA is induced by highly complex biochemical mechanisms, including the expression of genes encoding stress proteins (dehydrins), increased levels of sugars, enhanced antioxidant defense mechanisms, and changes in lipid compositions 12–14 CA increases the proportion of unsaturated fatty acids in cucumber seedlings, improves fluidity, and alleviates the damage of membrane peroxidation under cold stress by improving the activity of antioxidant enzymes and accumulating antioxidants in plants.…”

Section: Introductionmentioning

confidence: 99%

Cold acclimation alleviates cold stress-induced PSII inhibition and oxidative damage in tobacco leaves

Wei¹,

Chen²,

Wang³

et al. 2021

Plant Signaling & Behavior

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This study aimed to explore how cold acclimation (CA) modulates cold stress in tobacco leaves and reveal the relationship between CA and cold stress resistance, and the mechanism of CA-induced plant resistance to cold stress. This study examined the effects of CA treatment (at 8–10℃ for 2 d) on the cold tolerance of tobacco leaves under 4°C cold stress treatment using seedlings without CA treatment as the control (NA). In both CA and NA leaves, cold stress treatment resulted in a decrease in maximum photochemical efficiency of PSII ( F v /F m ), increase in relative variable fluorescence ( V J ) at 2 ms on the standardized OJIP curve, inhibition of PSII activity, and impairment of electron transfer on the acceptor side. Besides increasing the malondialdehyde (MDA) content and electrolyte leakage rate, the cold stress exacerbated the degree of membrane peroxidation. The CA treatment also induced the accumulation of reactive oxygen species (ROS), including superoxide anion (O 2 · − ) and H 2 O 2, and increased the activities of antioxidant enzymes, such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbic acid peroxidase (APX). The CA treatment also enhanced the accumulation of soluble sugar (SS) and soluble protein (SP), cyclic electron flow (CEF), and the proportion of regulatory energy dissipation Y(NPQ). Moreover, CA+ cold stress treatment significantly reduced CEF and Y(NPQ) in tobacco leaves than under NA+ cold stress treatment, thus significantly alleviating the degree of PSII photoinhibition. In conclusion, CA treatment significantly alleviated PSII photoinhibition and oxidative damage in tobacco leaves under cold stress treatment. Improvement in cold resistance of tobacco leaves is associated with the induction of antioxidant enzyme activity, accumulation of osmoregulation substances, and initiation of photoprotective mechanisms.

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Overexpression of ICE1, a Regulator of Cold-Induced Transcriptome, Confers Cold Tolerance to Transgenic Zoysia japonica

Cited by 23 publications

References 57 publications

Mechanistic Basis of Silicon Mediated Cold Stress Tolerance in Alfalfa (Medicago sativa L.)

Mechanistic Basis of Silicon Mediated Cold Stress Tolerance in Alfalfa (Medicago sativa L.)

A Survey of Enhanced Cold Tolerance and Low-Temperature-Induced Anthocyanin Accumulation in a Novel Zoysia japonica Biotype

Cold acclimation alleviates cold stress-induced PSII inhibition and oxidative damage in tobacco leaves

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