Overexpression of IAA1 with domain II mutation impairs cell elongation and cell division in inflorescences and leaves of Arabidopsis

Ku, Su-Jin; Park, Jin‐Young; Ha, Suk-Bong; Kim, Jungmook

doi:10.1016/j.jplph.2008.07.006

Cited by 27 publications

(11 citation statements)

References 26 publications

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“…S4B) (9). We also found that PHB was an indirect target of SHR (33), and as shown in a time course experiment (9), INDOLE-3-ACETIC ACID INDUCIBLE 1 (IAA1) was also downstream of SHR (8) (34). Unfortunately, we were not able to test NAC076 and ACETIC ACID INDUCIBLE 29 (IAA29) since the Agilent chip did not have probes covering the promoter region of such genes (9).…”

Section: Resultssupporting

confidence: 60%

An inference approach combines spatial and temporal gene expression data to predict gene regulatory networks in Arabidopsis stem cells

Balaguer

Clark

et al. 2017

Preprint

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Section: Resultssupporting

confidence: 60%

An inference approach combines spatial and temporal gene expression data to predict gene regulatory networks in Arabidopsis stem cells

Balaguer

Clark

et al. 2017

Preprint

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“…Of some 20 Aux/IAA proteins that have been tested to date, including 16 from Arabidopsis, all functioned as transcriptional repressors when expressed from effector plasmids in protoplast transfection assays (Ulmasov et al, 1997;Tiwari et al, 2001;Bargmann and Birnbaum, 2009). Consistent with the transfection results, targeted or constitutive expression of stabilized versions of IAA proteins in Arabidopsis plants resulted in suppression of auxin response genes and auxin-responsive growth and developmental responses (Fukaki et al, 2005;Kim et al, 2006;Nakamura et al, 2006;De Smet et al, 2007;Sato and Yamamoto, 2008;Ku et al, 2009;Li et al, 2009).…”

mentioning

confidence: 67%

Identical Amino Acid Substitutions in the Repression Domain of Auxin/Indole-3-Acetic Acid Proteins Have Contrasting Effects on Auxin Signaling

Tiwari

Hagen

et al. 2011

Plant Physiology

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Auxin/indole-3-acetic acid (Aux/IAA) proteins function as repressors of auxin response gene expression when auxin concentrations in a cell are low. At elevated auxin concentrations, these repressors are destroyed via the ubiquitin-proteasome pathway, resulting in derepression/activation of auxin response genes. Most Aux/IAA repressors contain four conserved domains, with one of these being an active, portable repression domain (domain I) and a second being an auxin-dependent instability domain (domain II). Here, we have analyzed the effects of amino acid substitutions in the repression domain of selected Aux/IAA proteins. We show that stabilized versions of Aux/IAA proteins with amino acid substitutions in domain I display contrasting phenotypes when expressed in transformed Arabidopsis (Arabidopsis thaliana) plants. An alanine-forleucine substitution in the LxLxL (where L is leucine and x is another amino acid) repression domain of IAA3, IAA6, or IAA19 confers enhanced auxin response gene expression and "high-auxin" phenotypes when expressed from the 35S or IAA19 promoter (as tested with IAA19) in transformed Arabidopsis plants. In marked contrast, a single alanine-for-leucine substitution in domain I of IAA12 or IAA17 confers repression of auxin response genes and "low-auxin" phenotypes. These results point to intrinsic differences in the repression domain(s) of IAA proteins and suggest that some IAA proteins have stronger or more complex repression domains than others.

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“…DEX treatment of this transgenic Arabidopsis evoked dramatic auxin-related phenotypes and repressed the auxin induction of a variety of Aux/IAA genes (Park et al, 2002;Ku et al, 2009). We previously assessed the effects of DEX-inducible iaa1 on auxinregulated gene expression, focusing on early genes, with the Affymetrix full genome array and subsequently identified a transcriptome downstream of iaa1 during the auxin response (Lee et al, 2009).…”

Section: Resultsmentioning

confidence: 99%

LBD18/ASL20Regulates Lateral Root Formation in Combination withLBD16/ASL18Downstream ofARF7andARF19in Arabidopsis

et al. 2009

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The LATERAL ORGAN BOUNDARIES DOMAIN/ASYMMETRIC LEAVES2-LIKE (LBD/ASL) genes encode proteins harboring a conserved amino acid domain, referred to as the LOB (for lateral organ boundaries) domain. While recent studies have revealed developmental functions of some LBD genes in Arabidopsis (Arabidopsis thaliana) and in crop plants, the biological functions of many other LBD genes remain to be determined. In this study, we have demonstrated that the lbd18 mutant evidenced a reduced number of lateral roots and that lbd16 lbd18 double mutants exhibited a dramatic reduction in the number of lateral roots compared with lbd16 or lbd18. Consistent with this observation, significant b-glucuronidase (GUS) expression in Pro LBD18 :GUS seedlings was detected in lateral root primordia as well as in the emerged lateral roots. Whereas the numbers of primordia of lbd16, lbd18, and lbd16 lbd18 mutants were similar to those observed in the wild type, the numbers of emerged lateral roots of lbd16 and lbd18 single mutants were reduced significantly. lbd16 lbd18 double mutants exhibited additively reduced numbers of emerged lateral roots compared with single mutants. This finding indicates that LBD16 and LBD18 may function in the initiation and emergence of lateral root formation via a different pathway. LBD18 was shown to be localized into the nucleus. We determined whether LBD18 functions in the nucleus using a steroid regulator-inducible system in which the nuclear translocation of LBD18 can be regulated by dexamethasone in the wild-type, lbd18, and lbd16 lbd18 backgrounds. Whereas LBD18 overexpression in the wild-type background induced lateral root formation to some degree, other lines manifested the growth-inhibition phenotype. However, LBD18 overexpression rescued lateral root formation in lbd18 and lbd16 lbd18 mutants without inducing any other phenotypes. Furthermore, we demonstrated that LBD18 overexpression can stimulate lateral root formation in auxin response factor7/19 (arf7 arf19) mutants with blocked lateral root formation. Taken together, our results suggest that LBD18 functions in the initiation and emergence of lateral roots, in conjunction with LBD16, downstream of ARF7 and ARF19.

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Overexpression of IAA1 with domain II mutation impairs cell elongation and cell division in inflorescences and leaves of Arabidopsis

Cited by 27 publications

References 26 publications

An inference approach combines spatial and temporal gene expression data to predict gene regulatory networks in Arabidopsis stem cells

An inference approach combines spatial and temporal gene expression data to predict gene regulatory networks in Arabidopsis stem cells

Identical Amino Acid Substitutions in the Repression Domain of Auxin/Indole-3-Acetic Acid Proteins Have Contrasting Effects on Auxin Signaling

LBD18/ASL20Regulates Lateral Root Formation in Combination withLBD16/ASL18Downstream ofARF7andARF19in Arabidopsis

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