Overexpression of <i>trans</i>‐Golgi network t‐<scp>SNARE</scp>s rescues vacuolar trafficking and <scp>TGN</scp> morphology defects in a putative tethering factor mutant

Yang, Xiaochen; Liao, Ching‐Yi; Tong, Jie; Bassham, Diane C.

doi:10.1111/tpj.14353

Cited by 10 publications

(10 citation statements)

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“…Interestingly, the TGN was disrupted and vesicle formation from the Golgi cisternae was affected in a tno1 mutant, and these defects were rescued by overexpression of either the Qa-SNARE SYP41 or the Qc-SNARE SYP61. These results supported the implication of the tethering factor TNO1 in vesicle fusion at the TGN (Yang et al, 2019).…”

Section: Breakthrough: Isolation Of Syp61 Tgn-derived Vesiclessupporting

confidence: 83%

Complex roles of Rabs and SNAREs in the secretory pathway and plant development: a never‐ending story

Martinière

Moreau

2020

Journal of Microscopy

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Membrane trafficking is critical for cell compartmentation which allows the maintenance of specialised environments required for specific cellular activities. To achieve this goal, cells need to tightly regulate vesicle transport between donor and acceptor compartments. Several different protein families are involved: among them the SNAREs (65 genes) and the small GTPases Rabs (57 genes) show the highest number of isoforms and will be in the spotlight. We will focus on the roles of these proteins in the ER-Golgi-plasma membrane pathway to illustrate how Rabs and SNAREs are involved to achieve specific set of functions.

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Section: Breakthrough: Isolation Of Syp61 Tgn-derived Vesiclessupporting

confidence: 83%

Complex roles of Rabs and SNAREs in the secretory pathway and plant development: a never‐ending story

Martinière

Moreau

2020

Journal of Microscopy

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“…However, homolog genes in animal cells participate in: (i) the deactivation of Rab3 [14], a homolog of RabD in plants, (ii) interaction with ATG8 in autophagy processes [59,60], (iii) or activation of a RabGTPase of subfamily C, acting as a guanine exchange factor in degradative pathways and macro-autophagy [61]. Intriguingly, a RabD of plants (RABD2A) localizes with proteins associated with salt-stress tolerance capacity, such as EHD1 or SYP41 [62,63]. In a similar way to expression patterns of Rab3GAP1, Rab3GAP2, and RabGAP10 of S. lycopersicum , the autophagy is a mechanism rapidly induced by salt stress [64].…”

Section: Discussionmentioning

confidence: 99%

The RabGAP Gene Family in Tomato (Solanum lycopersicum) and Wild Relatives: Identification, Interaction Networks, and Transcriptional Analysis during Plant Development and in Response to Salt Stress

Madrid-Espinoza

Salinas-Cornejo

Ruíz-Lara

2019

Genes

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RabGTPase activating proteins (RabGAP) are responsible for directing the deactivation of vesicular trafficking master regulators associated to plant development, the RabGTPase proteins. Recently, RabGAPs were identified in Arabidopsis and rice, but studies were not yet reported in tomato. Herein, we identified 24 RabGAP-encoding genes in cultivated tomato (Solanum lycopersicum) and its wild relative genomes (Solanum pimpinellifolium and Solanum pennellii). We analyzed them based on their exon-intron structures, conserved protein motifs, putative subcellular localizations, phylogenetic and gene duplications analyses, interaction networks, and gene expression patterns in tomato. Phylogenetic relationship analysis also indicated that RabGAP family is classified into seven subclasses, of which subclasses I and II are plant-exclusive. Furthermore, segmental duplication events and positive evolutionary forces are associated with the maintenance of the number and function of their members. On the other hand, the protein–protein interaction networks on tomato suggested that members of subclasses I, II, and III could be associated to endocytic traffic routes. In addition, the qRT-PCR experiments in S. lycopersicum and Solanum chilense exposed to a salt stress treatment validated the differential expression patterns of 20 RabGAP genes in different tissues, development stages, and stress conditions obtained through extensive microarray-based analyses. This work suggests the critical role of RabGAP family in the context of intracellular vesicular trafficking in tomato, particularly under conditions of abiotic stress. It also contributes to the breeding programs associated with the development of crops tolerant to salt stress.

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“…Surprisingly, there were only three SSTF DEGs in ERY dataset, among 45 genes analyzed in both datasets (Additional file 8: Table S4 and Additional file 12: Data S1). Two of those genes showed similar FC in both cultivars while the expression of the third one, the recently characterized TDR INTERACTING PROTEIN 3 ( TIP3 ) (Yang et al 2019), was more induced by cold stress in ERY than in S.AND cultivar (Additional file 8: Table S4 and Additional file 12: Data S1).…”

Section: Resultsmentioning

confidence: 99%

Early cold stress responses in post-meiotic anthers from tolerant and sensitive rice cultivars

González-Schain

Roig‐Villanova

Kater

2019

Rice

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BackgroundRice grain production is susceptible to a changing environment that imposes both biotic and abiotic stress conditions. Cold episodes are becoming more frequent in the last years and directly affect rice yield in areas with a temperate climate. Rice is particularly susceptible to cold stress during the reproductive phase, especially in anthers during post-meiotic stages which, in turn, affect pollen production. However, a number of rice cultivars with a certain degree of tolerance to cold have been described, which may represent a good breeding resource for improvement of susceptible commercial varieties. Plants experiencing cold stress activate a molecular response in order to reprogram many metabolic pathways to face these hostile conditions.ResultsHere we performed RNA-seq analysis using cold-stressed post-meiotic anther samples from a cold-tolerant, Erythroceros Hokkaido (ERY), and a cold-susceptible commercial cultivar Sant’Andrea (S.AND). Both cultivars displayed an early common molecular response to cold, although the changes in expression levels are much more drastic in the tolerant one. Comparing our datasets, obtained after one-night cold stress, with other similar genome-wide studies showed very few common deregulated genes, suggesting that molecular responses in cold-stressed anthers strongly depend on conditions and the duration of the cold treatments. Cold-tolerant ERY exhibits specific molecular responses related to ethylene metabolism, which appears to be activated after cold stress. On the other hand, S.AND cold-treated plants showed a general downregulation of photosystem I and II genes, supporting a role of photosynthesis and chloroplasts in cold responses in anthers, which has remained elusive.ConclusionsOur study revealed that a number of ethylene-related transcription factors, as putative master regulators of cold responses, were upregulated in ERY providing promising candidates to confer tolerance to susceptible cultivars. Our results also suggest that the photosynthesis machinery might be a good target to improve cold tolerance in anthers. In summary, our study provides valuable candidates for further analysis and molecular breeding for cold-tolerant rice cultivars.

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Overexpression of trans‐Golgi network t‐SNAREs rescues vacuolar trafficking and TGN morphology defects in a putative tethering factor mutant

Cited by 10 publications

References 94 publications

Complex roles of Rabs and SNAREs in the secretory pathway and plant development: a never‐ending story

Complex roles of Rabs and SNAREs in the secretory pathway and plant development: a never‐ending story

The RabGAP Gene Family in Tomato (Solanum lycopersicum) and Wild Relatives: Identification, Interaction Networks, and Transcriptional Analysis during Plant Development and in Response to Salt Stress

Early cold stress responses in post-meiotic anthers from tolerant and sensitive rice cultivars

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