Overexpression of<scp>CaWRKY27</scp>, a subgroup<scp>IIe WRKY</scp>transcription factor ofCapsicum annuum, positively regulates tobacco resistance toRalstonia solanacearuminfection

Dang, Fengfeng; Wang, Yuna; She, Jianju; Lei, Yang; Liu, Zhiqin; Eulgem, Thomas; Liu, Yan; Lin, Jin-Jia; Yu, Lu; Lei, Dan; Guan, Deyi; Li, Xia; Yuan, Qian; He, Shuilin

doi:10.1111/ppl.12093

Cited by 126 publications

(99 citation statements)

References 70 publications

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“…Since its first identification in sweet potato56, WRKY has been characterized in various plant species and shown to be involved in many biological processes, including biotic and abiotic stress responses789 and plant–pathogen interactions910. In tomato, the effector-triggered immunity against Pseudomonas syringae pv.…”

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confidence: 99%

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Putative WRKYs associated with regulation of fruit ripening revealed by detailed expression analysis of the WRKY gene family in pepper

Cheng

Ahammed

et al. 2016

Sci Rep

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WRKY transcription factors play important roles in plant development and stress responses. Here, global expression patterns of pepper CaWRKYs in various tissues as well as response to environmental stresses and plant hormones were systematically analyzed, with an emphasis on fruit ripening. The results showed that most CaWRKYs were expressed in at least two of the tissues tested. Group I, a subfamily of the entire CaWRKY gene family, had a higher expression level in vegetative tissues, whereas groups IIa and III showed relatively lower expression levels. Comparative analysis showed that the constitutively highly expressed WRKY genes were conserved in tomato and pepper, suggesting potential functional similarities. Among the identified 61 CaWRKYs, almost 60% were expressed during pepper fruit maturation, and the group I genes were in higher proportion during the ripening process, indicating an as-yet unknown function of group I in the fruit maturation process. Further analysis suggested that many CaWRKYs expressed during fruit ripening were also regulated by abiotic stresses or plant hormones, indicating that these CaWRKYs play roles in the stress-related signaling pathways during fruit ripening. This study provides new insights to the current research on CaWRKY and contributes to our knowledge about the global regulatory network in pepper fruit ripening.

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confidence: 99%

“…Recently, different types of transcription factors, including WRKY, MYB, and AP2/EREBP, have been induced during the ripening of pepper fruits31. Several CaWRKY s have also been reported to be involved in stress responses in pepper91032. However, there are a very few studies on the potential roles of CaWRKY proteins in fruit maturation.…”

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confidence: 99%

Putative WRKYs associated with regulation of fruit ripening revealed by detailed expression analysis of the WRKY gene family in pepper

Cheng

Ahammed

et al. 2016

Sci Rep

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“…WRKY transcription factors have been proven to play important roles in plant defence responses to attacks by several pathogens. CaWRKY27, a WRKY protein from pepper ( Capsicum annum ) positively regulates the stress resistance response to Ralstonia solanacearum infection through modulation of SA‐, JA‐ and ET‐mediated signaling pathways in tobacco ( Nicotiana tabacum ) (Dang et al ). Overexpression of the cotton ( Gossypium hirsutum ) genes GhWRKY39‐1 and GhWRKY40 in tobacco regulates its resistance response to R. solanacearum (Shi et al ).…”

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confidence: 99%

WRKY transcription factors in plant responses to stresses

Jiang

et al. 2017

JIPB

695

469

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The WRKY gene family is among the largest families of transcription factors (TFs) in higher plants. By regulating the plant hormone signal transduction pathway, these TFs play critical roles in some plant processes in response to biotic and abiotic stress. Various bodies of research have demonstrated the important biological functions of WRKY TFs in plant response to different kinds of biotic and abiotic stresses and working mechanisms. However, very little summarization has been done to review their research progress. Not just important TFs function in plant response to biotic and abiotic stresses, WRKY also participates in carbohydrate synthesis, senescence, development, and secondary metabolites synthesis. WRKY proteins can bind to W-box (TGACC (A/T)) in the promoter of its target genes and activate or repress the expression of downstream genes to regulate their stress response. Moreover, WRKY proteins can interact with other TFs to regulate plant defensive responses. In the present review, we focus on the structural characteristics of WRKY TFs and the research progress on their functions in plant responses to a variety of stresses.

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“…By ChIP assay, it was found that, amongst the three typical W‐boxes, only the W3‐box appeared to be bound by CaWRKY40, which was further supported by the data from MST analysis. However, the W3‐box was not found to be bound by CaWRKY27 and CaWRKY58, the two other pepper WRKY TFs implicated in pepper immunity against R. solanacearum by our previous studies (Dang et al ., ; Wang et al ., ); this suggests that the binding of CaWRKY40 to the W3‐box containing the CaC3H14 promoter is selective; however, its possible binding by other WRKY TFs cannot be ruled out at present. We also observed that, on R. solanacearum infection, pepper plants transiently overexpressing CaWRKY40 exhibited significantly enhanced CaC3H14 transcription, whereas TRV:: CaWRKY40 plants exhibited significantly down‐regulated CaC3H14 transcript abundance, compared with control plants.…”

Section: Discussionmentioning

confidence: 99%

CaC3H14 encoding a tandem CCCH zinc finger protein is directly targeted by CaWRKY40 and positively regulates the response of pepper to inoculation by Ralstonia solanacearum

Qiu

Lei

Yang

et al. 2018

Molecular Plant Pathology

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Tandem CCCH zinc finger (TZnF) proteins have been implicated in plant defence, but their role in pepper (Capsicum annuum) is unclear. In the present study, the role of CaC3H14, a pepper TZnF protein, in the immune response of pepper plants to Ralstonia solanacearum infection was characterized. When fused to the green fluorescent protein, CaC3H14 was localized exclusively to the nuclei in leaf cells of Nicotiana benthamiana plants transiently overexpressing CaC3H14. Transcript abundance of CaC3H14 was up-regulated by inoculation with R. solanacearum. Virus-induced silencing of CaC3H14 increased the susceptibility of the plants to R. solanacearum and down-regulated the genes associated with the hypersensitive response (HR), specifically HIR1 and salicylic acid (SA)-dependent PR1a. By contrast, silencing resulted in the up-regulation of jasmonic acid (JA)-dependent DEF1 and ethylene (ET) biosynthesis-associated ACO1. Transient overexpression of CaC3H14 in pepper triggered an intensive HR, indicated by cell death and hydrogen peroxide (H O ) accumulation, up-regulated PR1a and down-regulated DEF1 and ACO1. Ectopic overexpression of CaC3H14 in tobacco plants significantly decreased the susceptibility of tobacco plants to R. solanacearum. It also up-regulated HR-associated HSR515, immunity-associated GST1 and the SA-dependent marker genes NPR1 and PR2, but down-regulated JA-dependent PR1b and ET-dependent EFE26. The CaC3H14 promoter and was bound and its transcription was up-regulated by CaWRKY40. Collectively, these results indicate that CaC3H14 is transcriptionally targeted by CaWRKY40, is a modulator of the antagonistic interaction between SA and JA/ET signalling, and enhances the defence response of pepper plants to infection by R. solanacearum.

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Overexpression ofCaWRKY27, a subgroupIIe WRKYtranscription factor ofCapsicum annuum, positively regulates tobacco resistance toRalstonia solanacearuminfection

Cited by 126 publications

References 70 publications

Putative WRKYs associated with regulation of fruit ripening revealed by detailed expression analysis of the WRKY gene family in pepper

Putative WRKYs associated with regulation of fruit ripening revealed by detailed expression analysis of the WRKY gene family in pepper

WRKY transcription factors in plant responses to stresses

CaC3H14 encoding a tandem CCCH zinc finger protein is directly targeted by CaWRKY40 and positively regulates the response of pepper to inoculation by Ralstonia solanacearum

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