Overexpression of<i>OsCYP19-4</i>increases tolerance to cold stress and enhances grain yield in rice (<i>Oryza sativa</i>)

Yoon, Dae Hwa; Lee, Sang Sook; Park, Hyun Ji; Lyu, Jae Il; Chong, Won Seog; Liu, Jang Ryol; Kim, Beom-Gi; Ahn, Jinhyun; Cho, Hye Sun

doi:10.1093/jxb/erv421

Cited by 49 publications

(43 citation statements)

References 54 publications

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“…In addition, protein-protein interaction analysis showed that the psbQ-like protein 1 was interacted with another up-regulated protein PPI (A0A0K9RJJ3 |peptidyl-prolyl cis-trans isomerase fkbp16-4) ( Figure 4a). The same up-regulation has been shown in other studies [19] which imply that plants may respond to salt stress by accumulating PPI to accelerate protein synthesis. Another up-regulated protein A0A0K9QN40 is thioredoxin Y1 that has the ability to regulate the activity of photosynthetic enzymes [20] .…”

Section: Analysis Of the Daps Response To Salt Stress In Chloroplastsupporting

confidence: 84%

iTRAQ protein profile analysis of leaves and roots of sugar beet (Beta vulgaris) differing in response to salt stress

Cui

Cheng

et al. 2020

Preprint

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Background Salinity is one of the most serious threat to agriculture worldwide. Sugar beet is an important sugar-yielding crop and has a certain tolerance to salt. However, the molecular mechanism of salt tolerance in beta vulgaris are poorly understood. Proteomics can provide a new perspective and deeper understanding for the research of beet salt-tolerant. Results Here, leaves and roots were used to identify the differentially abundant protein species between salt-stress and control conditions in beta vulgaris. As a result, 70 and 76 DAPs were identified in leaves and roots, respectively. The functions were determined for the classification of the DAPs, mainly involved in cellular processes, environmental information processing, genetic information processing and metabolism. These processes can work cooperatively to reconstruct the favorable equilibrium of physiological and cellular homeostasis under salt stress. Some candidate DAPs are closely related to salt resistance such as choline monooxygenase, betaine aldehyde dehydrogenase, glutathione S-transferase (GST) and F-type H+-transporting ATPase. The expressional pattern of 10 DAPs encoding genes were consistent with the iTRAQ data. Conclusions Our results demonstrated that during adaptation of beet to salt stress, leaves and roots have distinct mechanisms of molecular metabolism regulation. This study provided some significative insights into the molecular mechanism underlying the response of higher plant to salt stress, and identified some candidate proteins against salt stress.

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Section: Analysis Of the Daps Response To Salt Stress In Chloroplastsupporting

confidence: 84%

iTRAQ protein profile analysis of leaves and roots of sugar beet (Beta vulgaris) differing in response to salt stress

Cui

Cheng

et al. 2020

Preprint

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“…In previous studies, we showed that several plant IMMs function in various subcellular organelles, such as cytosol, apoplast, chloroplast, and Golgi apparatus, as positive regulators of the abiotic stress response (Park et al , ; Seok et al , ; Lee et al , ; Yoon et al , ; Park et al , ). In rice, OsFKBP20‐1b is the most inducible IMM under water stress (Ahn et al , ), and is also responsive to other abiotic stresses (Figure ).…”

Section: Discussionmentioning

confidence: 99%

OsFKBP20‐1b interacts with the splicing factor OsSR45 and participates in the environmental stress response at the post‐transcriptional level in rice

et al. 2020

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Sessile plants have evolved distinct mechanisms to respond and adapt to adverse environmental conditions through diverse mechanisms including RNA processing. While the role of RNA processing in the stress response is well understood for Arabidopsis thaliana, limited information is available for rice (Oryza sativa).Here, we show that OsFKBP20-1b, belonging to the immunophilin family, interacts with the splicing factor OsSR45 in both nuclear speckles and cytoplasmic foci, and plays an essential role in post-transcriptional regulation of abiotic stress response. The expression of OsFKBP20-1b was highly upregulated under various abiotic stresses. Moreover genetic analysis revealed that OsFKBP20-1b positively affected transcription and pre-mRNA splicing of stress-responsive genes under abiotic stress conditions. In osfkbp20-1b loss-of-function mutants, the expression of stress-responsive genes was downregulated, while that of their splicing variants was increased. Conversely, in plants overexpressing OsFKBP20-1b, the expression of the same stress-responsive genes was strikingly upregulated under abiotic stress. In vivo experiments demonstrated that OsFKBP20-1b directly maintains protein stability of OsSR45 splicing factor. Furthermore, we found that the plant-specific OsFKBP20-1b gene has uniquely evolved as a paralogue only in some Poaceae species. Together, our findings suggest that OsFKBP20-1b-mediated RNA processing contributes to stress adaptation in rice.OsFKBP20-1b is involved in pre-mRNA processing under abiotic stress 999 Subcellular localization and co-localizationThe full-length coding sequence (CDS) of OsFKBP20-1b was cloned into the pCAMBIA1302 binary vector, and the recombinant

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“…Rice (Oryza sativa), as a dietary staple for almost two-thirds of the world's population, is one of the most important agricultural crops. Recently, some genes have been used successfully to improve the tolerance of rice to stresses [23][24][25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and function analysis of the rice OsDUF946 family

et al. 2017

Biotechnology & Biotechnological Equipment

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The domain of unknown function DUF946 family consists of plant proteins with an average length of around 239 residues that have no characterized function. The gene family needs deeper characterization and functional analysis of its members. In this study, we report five OsDUF946 (OsDUF946.1-OsDUF946.5) family members in rice Nipponbare with three distinct motifs. All the OsDUF946 proteins were classified into three major groups (I, II, III) by phylogenetic analysis. Realtime polymerase chain reaction showed that the expression patterns of the five OsDUF946 family members were different in 15 different rice tissues as well as under various stress conditions and abscisic acid treatment. The expression of OsDUF946.1 was significantly downregulated under salt, cold and heat stress, while the expression of OsDUF946.4 and OsDUF946.5 was significantly upregulated under drought and salt conditions. Overexpression of OsDUF946.4 in Escherichia coli significantly improved the resistance to salt and drought, while overexpression of OsDUF946.5 in E. coli did not have such an effect. The obtained results provide a starting point for further research into the function of the OsDUF946 family in rice.

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Overexpression ofOsCYP19-4increases tolerance to cold stress and enhances grain yield in rice (Oryza sativa)

Abstract: HighlightOsCYP19-4 is a novel apoplastic immunophilin that plays a role in developmental acclimation and cold stress, probably via regulation of auxin transport.

Cited by 49 publications

References 54 publications

iTRAQ protein profile analysis of leaves and roots of sugar beet (Beta vulgaris) differing in response to salt stress

iTRAQ protein profile analysis of leaves and roots of sugar beet (Beta vulgaris) differing in response to salt stress

OsFKBP20‐1b interacts with the splicing factor OsSR45 and participates in the environmental stress response at the post‐transcriptional level in rice

Molecular characterization and function analysis of the rice OsDUF946 family

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