Overexpression of <i>LSH1</i>, a member of an uncharacterised gene family, causes enhanced light regulation of seedling development

Zhao, Li; Nakazawa, Miki; Takase, Tomoyuki; Manabe, Katsushi; Kobayashi, Masatomo; Seki, Motoaki; Shinozaki, Kazuo; Matsui, Minami

doi:10.1111/j.1365-313x.2003.01993.x

Cited by 79 publications

(82 citation statements)

References 53 publications

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“…LSH1 was identified by activation tagging; LSH1-D exhibits a dominant short hypocotyl phenotype in response to red, blue, and far-red light. Ten LSH homologs have been described (26); OBO1 corresponds to LSH3 (Figs. S1 and S2).…”

Section: Resultsmentioning

confidence: 99%

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ORGAN BOUNDARY1 defines a gene expressed at the junction between the shoot apical meristem and lateral organs

Cho

Zambryski

2011

Proc. Natl. Acad. Sci. U.S.A.

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We identify a gene, ORGAN BOUNDARY1 (OBO1), by its unique pattern of enhancer-driven GFP expression at the boundaries between the apical meristems and lateral organs in Arabidopsis embryos, seedlings, and mature plants. OBO1 also is expressed at the root apical meristem and in distinct cell files surrounding this area. OBO1 is one of a 10-member plant-specific gene family encoding a single small domain (133 amino acids) with unknown function. One member of this gene family, OBO2, is identical to a previously studied gene, LIGHT-SENSITIVE HYPOCOTYL1. Overexpression of OBO1 causes an abnormal number and size of petals and petalstamen fusions. The patterns of OBO1 gene expression are distinct but overlap with other genes involved in boundary formation in the Arabidopsis shoot apical meristem, including CUP-SHAPED COTYLEDON, LATERAL ORGAN BOUNDARIES, BLADE-ON-PETIOLE, ASYMMETRIC LEAVES, and LATERAL ORGAN FUSION. Nuclear localization of OBO1 suggests that it might act with one or more of the transcription factors encoded by the foregoing genes. Ablation of the specific cells expressing OBO1 leads to loss of the shoot apical meristem and lateral organs. Thus, the cells expressing OBO1 are important for meristem maintenance and organogenesis in Arabidopsis.

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Section: Resultsmentioning

confidence: 99%

“…OBO2 has been previously identified as LIGHT-DEPENDENT SHORT HYPOCOTYL 1 (LSH1) (26). LSH1 was identified by activation tagging; LSH1-D exhibits a dominant short hypocotyl phenotype in response to red, blue, and far-red light.…”

Section: Resultsmentioning

confidence: 99%

ORGAN BOUNDARY1 defines a gene expressed at the junction between the shoot apical meristem and lateral organs

Cho

Zambryski

2011

Proc. Natl. Acad. Sci. U.S.A.

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“…The ALOG domain was defined based on the conserved amino acid sequence in the Arabidopsis LIGHT-DEPENDENT SHORT HYPOCOTYLS1 (LSH1) and rice G1 genes Zhao et al, 2004). The ALOG domain is distributed throughout land plants and is highly conserved .…”

Section: Discussionmentioning

confidence: 99%

A role for <i>TRIANGULAR HULL1</i> in fine-tuning spikelet morphogenesis in rice

et al. 2014

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The lemma and palea, which enclose the pistil, stamens, and lodicules, are the most conspicuous organs in the rice spikelet. We isolated a mutant line (ng6569) in which the lemma and palea were narrower than those of the wild type, and found that the mutant had a defect in TRIANGULAR HULL1 (TH1), which encodes a nuclear protein with an ALOG domain. Detailed morphological analysis indicated that the th1 mutation caused a reduction in the size of tubercles, which are convex structures on the surface of the lemma and palea. This reduction was more pronounced in the apical region of the lemma than in the basal region, resulting in the formation of a beak-like spikelet. By contrast, the number of tubercle rows and their spatial distribution on the lemma were not affected in the th1 mutant. Thus, the TH1 gene seems to be involved in fine-tuning the morphogenesis of the lemma and palea. In situ hybridization analysis revealed that TH1 was highly expressed in the primordia of the lemma and palea, but only weakly expressed in the primordia of the sterile lemma and rudimentary glume. We then examined the effect of th1 mutation on the lemma-like structure formed in the long sterile lemma/glume1 (g1) and extra glume1 (eg1) mutants. The result showed that the th1 mutation strongly affected the morphology of the extra lemma of eg1, but had no significant effect on the transformed lemma of g1.

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“…3). The only gene functionally characterized so far in this family is LIGHT-DEPENDENT SHORT HYPOCOTYLS1 (LSH1), which is involved in phytochrome-dependent light signaling in Arabidopsis (31). Here, we named DUF640 the ALOG (Arabidopsis LSH1 and Oryza G1) domain.…”

Section: G1 Encodes a Unique Protein With An Uncharacterized Domainmentioning

confidence: 99%

The homeotic gene long sterile lemma ( G1 ) specifies sterile lemma identity in the rice spikelet

Yoshida

Suzaki

Tanaka

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

150

175

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The mechanism of floral organ specification is principally conserved in angiosperms, as demonstrated by the ABC model. By contrast, mechanisms that regulate the development of organs or structures specific to a group of species remain unclear. Grasses have unique inflorescence units, comprising spikelets and florets. In the genus Oryza (rice), the single spikelet consists of a fertile floret subtended by a lemma and a palea, two sterile lemmas, and rudimentary glumes. Each sterile lemma is a tiny glume-like organ with a smooth surface. Here, we have examined a long sterile lemma1 (g1) mutant, in which the sterile lemma is enlarged like the lemma. Detailed phenotypic analysis reveals that the large sterile lemma in the g1 mutant appears to be caused by homeotic transformation of the sterile lemma into a lemma, suggesting that G1 is involved in the repression of lemma identity to specify the sterile lemma. Gene isolation reveals that G1 is a member of a plant-specific gene family that encodes proteins with a previously uncharacterized domain, named here ALOG (Arabidopsis LSH1 and Oryza G1). G1 mRNA is expressed in sterile lemma primordia throughout their development, and G1 protein is localized in the nucleus. A trans-activation assay using the yeast GAL4 system suggests that G1 is involved in transcriptional regulation. Repression of lemma identity by G1 is consistent with a hypothesis proposed to explain the morphological evolution of rice spikelets. We also show that a wild rice species, Oryza grandiglumis, that forms large sterile lemmas has serious mutations in the G1 gene.flower ͉ grass ͉ Oryza ͉ ALOG ͉ morphological evolution A lthough angiosperms produce diverse forms of flowers, from beautiful and entomophilous to inconspicuous and anemophilous, the genetic programs directing floral development are fundamentally conserved. Floral organ specification is explained by the ABC model, and genes constituting this model are likely to be functionally conserved in a wide range of flowering plants (1-3). In contrast to our deep understanding of floral organs common to many angiosperms, genes that regulate the development of floral organs and/or flower-associated structures distinctive to a group of plant species remain poorly elucidated.Grass species, such as Oryza sativa (rice) and Zea mays (maize), bear a unique inflorescence consisting of spikelets and florets (4, 5). Each spikelet produces a defined number of florets depending on species, and is subtended by a pair of glumes. The floret comprises the flower proper (carpels, stamens, and lodicules) and a pair of additional structures (a palea and a lemma) that subtend the flower. The lodicule, an organ homologous to the petal in ordinary flowers, is small and semitransparent, and acts to open the palea and lemma for anthesis. Molecular genetic studies in rice and maize have revealed that the function of B class MADS-box genes is conserved in grasses: these genes specify the identities of the lodicule and stamen similar to the way in which B class genes specify t...

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Overexpression of LSH1, a member of an uncharacterised gene family, causes enhanced light regulation of seedling development

Cited by 79 publications

References 53 publications

ORGAN BOUNDARY1 defines a gene expressed at the junction between the shoot apical meristem and lateral organs

ORGAN BOUNDARY1 defines a gene expressed at the junction between the shoot apical meristem and lateral organs

A role for <i>TRIANGULAR HULL1</i> in fine-tuning spikelet morphogenesis in rice

The homeotic gene long sterile lemma ( G1 ) specifies sterile lemma identity in the rice spikelet

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