Overexpression of <i>AtLEAFY</i> Accelerates Flowering in <i>Brassica juncea</i>

Roy, Suchandra Deb; Saxena, Mukesh; Bhalla‐Sarin, Neera

doi:10.2135/cropsci2008.03.0118

Cited by 13 publications

(6 citation statements)

References 35 publications

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“…This is quite similar to phenotypes under constitutive LFY ortholog expression in Arabidopsis 27, Gloxinia 29, Brassica juncea 30, and Nicotiana tabacum 28. Constitutive LFY expression driven by the CaMV35S promoter attenuates development of both vegetative and inflorescence phases, inducing the production of terminal flowers on Arabidopsis primary shoots27.…”

Section: Discussionsupporting

confidence: 62%

“…Additionally, LFY overexpression accelerates flowering in a variety of other plants, including tobacco28, rice24, S inningia speciosa 29, Brassica juncea 30, and Citrus 31. In Arabidopsis lfy mutants, FM development is dramatically delayed with late flowering32, flower buds are surrounded by extra bracts, and the early flowers are completely transformed into inflorescence shoots.…”

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confidence: 99%

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An ortholog of LEAFY in Jatropha curcas regulates flowering time and floral organ development

Tang

Tao

et al. 2016

Sci Rep

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Jatropha curcas seeds are an excellent biofuel feedstock, but seed yields of Jatropha are limited by its poor flowering and fruiting ability. Thus, identifying genes controlling flowering is critical for genetic improvement of seed yield. We isolated the JcLFY, a Jatropha ortholog of Arabidopsis thaliana LEAFY (LFY), and identified JcLFY function by overexpressing it in Arabidopsis and Jatropha. JcLFY is expressed in Jatropha inflorescence buds, flower buds, and carpels, with highest expression in the early developmental stage of flower buds. JcLFY overexpression induced early flowering, solitary flowers, and terminal flowers in Arabidopsis, and also rescued the delayed flowering phenotype of lfy-15, a LFY loss-of-function Arabidopsis mutant. Microarray and qPCR analysis revealed several flower identity and flower organ development genes were upregulated in JcLFY-overexpressing Arabidopsis. JcLFY overexpression in Jatropha also induced early flowering. Significant changes in inflorescence structure, floral organs, and fruit shape occurred in JcLFY co-suppressed plants in which expression of several flower identity and floral organ development genes were changed. This suggests JcLFY is involved in regulating flower identity, floral organ patterns, and fruit shape, although JcLFY function in Jatropha floral meristem determination is not as strong as that of Arabidopsis.

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Section: Discussionsupporting

confidence: 62%

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confidence: 99%

An ortholog of LEAFY in Jatropha curcas regulates flowering time and floral organ development

Tang

Tao

et al. 2016

Sci Rep

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“…Overexpression of VpLFY2 causes precocious owering in Arabidopsis [28]. Similar results were also found in Brassica juncea [29], Jatropha [15], and mango [14]. Consistent with those studies, in the present study, 35S:DlLFY transgenic Arabidopsis lines OE10 and OE11 owered about 4 and 7 days earlier than WT.…”

Section: Discussionsupporting

confidence: 91%

Identification of LEAFY gene in longan involved in flower induction

Jue

Tang

et al. 2022

Preprint

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Backgroud: Flowering at the right time is a very important factor affecting the stable annual yield of longan. However, a lack of knowledge of the regulatory mechanism and key genes of longan flowering restricts the healthy development of the longan industry. Therefore, identifying the relevant genes and analyzing their regulatory mechanism are essential for scientific research and the development of the longan industry. Here, we isolated a LEAFY homologue gene from longan, DlLFY, and identified its function. Results: DlLFY contains a 1167 bp open reading frame and encodes 388 amino acids. The amino acid sequence has a typical LFY/FLO family domain. DlLFY was expressed in all the tested tissues, expect for leaf, pericarp, and ulp, with the highest expression occurring in flower buds. The expression of DlLFY was significant upregulated at the early flower induction stage in “SX” (“Shixia”). The results of subcellular localization and transactivation analysis show that DlLFY is a kind of typical transcript factor with transcriptional activator. Moreover, overexpression of DlLFY in Arabidopsis promoted early flowering and restrained growth, resulting in reduced plant height and number and area of rosette leaves of transgenic plants. DNA affinity purification sequencing (DAP-Seq) analysis showed that 13 flower-related genes corresponding to five homologous genes of Arabidopsis may be the binding sites and putative target genes. Among these five flower-related genes, only AtTFL1 was strongly inhibited in transgenic lines. Conclusion: Taken together, these results indicate that DlLFY plays a pivotal role in controlling longan flowering, possibly by negatively regulating TFL1.

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“…5). This result is in confirmation with the earlier reports in tomato, petunia and other legumes where there is role of leafy homologues in compound leaf development Hofer et al (1997) but is in contrast with the findings of Roy et al (2009) in B. juncea cv. Varuna.…”

Section: Phenotypic Analysissupporting

confidence: 92%

“…4). Early onset of flowering in transgenic plants is in concordance with the earlier reports of Leafy overexpression in different species tobacco Nilsson 1995), aspen (Wiegel andNilsson 1995), rice (He et al 2000), poplar (Rottmann et al 2000), citrus (Pena et al 2001) and B. juncea (Roy et al 2009). The PCR positive lines also showed different morphological defects.…”

Section: Phenotypic Analysissupporting

confidence: 89%

Ectopic expression of Atleafy in Brassica juncea cv. Geeta for early flowering

Sahni

Ganie

Narula

et al. 2013

Physiol Mol Biol Plants

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High temperature stress during pod filling severely affects the yield of Brassica juncea. Early flowering can evade the terminal heat stress and result in early maturity of the crop. In this study, a regeneration and transformation protocol has been standardized for B. juncea cv. Geeta. Hypocotyl from 5-day-old seedlings were used as explants. Of the various combinations of auxins and cytokinins tried along with Murashige and Skoog's (Physiol Plant 15:473-497, 1962) medium, MS + IAA (0.2 mg/l) + BA (3 mg/l) proved best for shoot regeneration with 89.9 % regeneration efficiency. To induce early flowering Leafy gene from Arabidopsis thaliana was transformed using Agrobacterium mediated transformation method. After 12 weeks transgenic plants showed flowering in vitro whereas their untransformed counterpart did not flower even after 16 weeks. The maximum transformation frequency was 4 %.

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Overexpression of AtLEAFY Accelerates Flowering in Brassica juncea

Cited by 13 publications

References 35 publications

An ortholog of LEAFY in Jatropha curcas regulates flowering time and floral organ development

An ortholog of LEAFY in Jatropha curcas regulates flowering time and floral organ development

Identification of LEAFY gene in longan involved in flower induction

Ectopic expression of Atleafy in Brassica juncea cv. Geeta for early flowering

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