Overexpression of hsa‑miR‑125a‑5p enhances proliferation, migration and invasion of head and neck squamous cell carcinoma cell lines by upregulating C‑C chemokine receptor typeï¿½7

Jin, Shan; Liu, Minda; Wu, Hong; Pang, Pai; Wang, Song; Li, Zhen‐Ning; Sun, Changfu; Liu, Fa‐Yu

doi:10.3892/ol.2018.8564

Cited by 12 publications

(12 citation statements)

References 31 publications

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“… 26 As for hsa-miR-125b, a previous study reported overexpression of hsa-miR-125b significantly enhanced the ability of cell proliferation, migration, and invasion in Head and Neck Squamous Cell Carcinoma, with upregulation of C-C Chemokine Receptor Type 7. 27 It also activates p53 and Induces Apoptosis in Lung Cancer Cells. 28 Besides, hsa-miR-125b may act as a cancer suppressor by regulating the BRCA1 signaling, and reintroduction of hsa-miR-125b analogs could be a potential adjunct treatment for advanced/chemoresistant breast cancers.…”

Section: Discussionmentioning

confidence: 99%

HS3ST2 and Its Related Molecules as Potential Biomarkers for Predicting Lymph Node Metastasis in Patients with Colorectal Cancer

Gao¹,

Shi²,

Shen³

2021

OTT

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Background Lymph node metastasis is a major cause of cancer-related death in patients with colorectal cancer (CRC), but current strategies are limited to predicting this clinical behavior. Our study aims to establish a lymph node metastasis prediction model based on miRNA and mRNA to improve the accuracy of prediction. Methods GSE56350, GSE70574, and GSE95109 were downloaded from the Gene Expression Omnibus (GEO) database and 569 colorectal cancer statistics were also downloaded from The Cancer Genome Atlas (TCGA) database. Differentially expressed miRNAs were calculated by using R software. Besides, gene ontology and enriched pathway analysis of target mRNAs were analyzed by using FunRich. Furthermore, the mRNA–miRNA network was constructed using Cytoscape software. Gene expression level was also detected by performing qRT-PCR (quantitative real-time PCR) in colorectal cancer and lymph node tissues. Results In total, 5 differentially expressed miRNAs were selected, and 34 mRNAs were identified after filtering. The research of KEGG indicated that mRNAs are enriched in many cancer pathways. Differentially expressed miRNAs were most enriched in the cytoplasm, nucleoside, transcription factor activity, and RNA binding. KEGG pathway analysis of these target genes was mainly enriched in 5 pathways including fatty acid elongation, MAPK signaling pathway, autophagy, signaling pathways regulating pluripotency of stem cells, and Th17 cell differentiation. The results of qRT-PCR indicated that hsa-miR-100 and hsa-miR-99a were differentially expressed in lymph node metastatic colorectal cancer tissues and lymph node non-metastasis tissues which all target HS3ST2. Besides, we also found they have a significant difference in colorectal cancer tissues compared with normal tissues. Conclusion By using microarray and bioinformatics analyses, differentially expressed miRNAs were identified and a complete gene network was constructed. To our knowledge, HS3ST2 and related molecules including hsa-miR-100 and hsa-miR-99a were firstly identified as potential biomarkers in the development of lymph node metastatic colorectal cancer.

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Section: Discussionmentioning

confidence: 99%

HS3ST2 and Its Related Molecules as Potential Biomarkers for Predicting Lymph Node Metastasis in Patients with Colorectal Cancer

Gao¹,

Shi²,

Shen³

2021

OTT

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“…miR-125a-5p, an extensively studied miRNA in tumors, has been identified to serve an inhibitor role in hepatitis B virus-related hepatocellular carcinoma (15). Jin et al (24) suggested that miR-125a-5p is associated with the clinical stages of head and neck squamous cell carcinoma, and the expression levels in low tumor stages (stages I and II) were higher compared with that in the high stages (stages III and IV). Furthermore, miR-125a-5p is a potential biomarker for acute ischemic stroke (25).…”

Section: Discussionmentioning

confidence: 99%

miRNA‑125a‑5p inhibits hepatocellular carcinoma cell proliferation and induces apoptosis by targeting TP53 regulated inhibitor of apoptosis 1 and Bcl‑2‑like‑2 protein

Ma³

2019

Exp Ther Med

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The present study aimed to investigate the role and underlying molecular mechanism of microRNA (miR)-125a-5p in hepatocellular carcinoma. The level of miR-125a-5p was detected using reverse transcription-quantitative polymerase chain reaction. TargetScan was used to investigate the association between miR-125a-5p and TP53-regulated inhibitor of apoptosis 1 (TRIAP1)/B cell lymphoma-2-like 2 protein (BCL2L2). Dual luciferase reporter assay was used to confirm this prediction. To investigate the role of miR-125a-5p in hepatocellular carcinoma (HCC) cells, miR-125a-5p was overexpressed in the human HCC cell line PLC/PRF/5 using miR-125a-5p mimics. Subsequently, cell proliferation, cell apoptosis and cell migration were studied using MTT assay, flow cytometry analysis and Transwell assay, respectively. Protein expression levels in the present study were measured by western blot analysis. Taken together, the present results suggested that miR-125a-5p was markedly downregulated in HCC cells. TRIAP1 and BCL2L2 were direct targets of miR-125a-5p and were upregulated in PLC/PRF/5 cells. miR-125a-5p upregulation inhibited PLC/PRF/5 cell viability and migration and induced cell apoptosis. In addition, miR-125a-5p overexpression increased the expression of caspase9 and apoptotic protease-activating factor 1. Notably, the present study revealed that all the effects on PLC/PRF/5 cells elicited by miR-125a-5p overexpression were eliminated by TRIAP1/BCL2L2 upregulation. In conclusion, miR-125a-5p was shown to be downregulated in hepatocellular carcinoma and its upregulation inhibited hepatocellular carcinoma cell growth and metastasis by targeting TRIAP1 and BCL2L2.

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“…High hsa-miR-107 expression was associated with poor clinicopathological parameters and prognosis in pancreatic ductal adenocarcinoma patients [53]. On the other hand, overexpression of hsa-miR-125a-5p significantly enhanced the ability of cell proliferation, migration and invasion in HNSCC, hsa-miR-125a-5p [54]. The hsa-miR-125a-5p induced apoptosis via a p53dependent pathway in human lung cancer cells [55].…”

Section: External Validation Of Survival Markers Using On Another 23 Cancersmentioning

confidence: 96%

Survival Marker miRNA-Mediated Subpathways of Breast Invasive Carcinoma Derived From Activity Profile

Ning

Zhao

et al. 2020

IEEE Access

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We not only collected 1102 samples form 3 different breast invasive carcinoma (BRCA) datasets, which included a TCGA dataset and 2 GEO datasets, but also collected 23 other cancer datasets, one of which include more than 100 samples. Using these datasets, we topologically inferred miRNAmediated subpathway activity profile, which integrated gene expression profiles, prior gene interaction information and target relations between miRNAs and genes, and topological information. Then we constructed the Global Directed Pathway Network (GDPN) with genes as nodes, and from 3 BRCA datasets and other 23 cancer datasets identified a set of miRNA-mediated subpathways that are survivalrelated risk markers. The results showed large activity values correlated with poor prognosis, such as hsa-miR-107 and hsa-miR-142-3p of BRCA datasets. We assessed the stability and robustness of the miRNAmediated subpathway survival markers with 2 GEO datasets and 23 external independent datasets. The results showed that the proposed method can significantly reduce noise from sequencing errors and samples heterogeneity by integrating pathway topological information, can break down the boundary of pathways and provide a new measure for detecting survival-related markers. The top miRNA-mediated subpathways are more reliable in stratifying high risk group and low risk group and selecting therapeutic strategies.

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Overexpression of hsa‑miR‑125a‑5p enhances proliferation, migration and invasion of head and neck squamous cell carcinoma cell lines by upregulating C‑C chemokine receptor typeï¿½7

Cited by 12 publications

References 31 publications

HS3ST2 and Its Related Molecules as Potential Biomarkers for Predicting Lymph Node Metastasis in Patients with Colorectal Cancer

HS3ST2 and Its Related Molecules as Potential Biomarkers for Predicting Lymph Node Metastasis in Patients with Colorectal Cancer

miRNA‑125a‑5p inhibits hepatocellular carcinoma cell proliferation and induces apoptosis by targeting TP53 regulated inhibitor of apoptosis 1 and Bcl‑2‑like‑2 protein

Survival Marker miRNA-Mediated Subpathways of Breast Invasive Carcinoma Derived From Activity Profile

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