Overexpression of gma-MIR394a confers tolerance to drought in transgenic Arabidopsis thaliana

Ni, Zhongfu; Hu, Zheng; Jiang, Qiyan; Zhang, Hui

doi:10.1016/j.bbrc.2012.09.055

Cited by 88 publications

(42 citation statements)

References 30 publications

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“…In A . thaliana , the gma‐miR394a gene positively modulates DS tolerance (Ni et al ). miR408 over‐expression caused an increase in drought tolerance in chickpeas ( Cicer arietinum ) (Hajyzadeh et al ).…”

Section: Discussionmentioning

confidence: 99%

Small RNA and degradome profiling reveals important roles for microRNAs and their targets in tea plant response to drought stress

Liu

et al. 2016

Physiologia Plantarum

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Tea (Camellia sinensis) is a popular beverage worldwide. Drought stress (DS) is a major constraint on the growth, yield and quality of tea plants. MicroRNAs (miRNAs) play important roles in plant responses to DS. We constructed eight small RNA libraries from the drought-tolerant 'Ningzhou 2' (NZ2) and drought-susceptible 'Zhuyeqi' (ZYQ) cultivars during four stages [control (CK), the fourth day of DS, the eighth day of DS and after recovery (RC)]. A total of 268 conserved and 62 novel miRNAs were identified using small RNA sequencing. In total, 139 (52.9%) and 96 (36.0%) conserved miRNAs were differentially expressed during the four stages (P ≤ 0.05) in NZ2 and ZYQ, respectively. A total of 814 predicted target genes were identified as differentially regulated by 199 miRNAs through degradome sequencing. Among them, 201 and 218 genes were specific to the NZ2 and ZYQ cultivars, respectively, and 395 were common to both cultivars. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed the biological roles of these targets and showed that some of the targets responded to DS in a stress- and cultivar-dependent manner. Correlated expression patterns between miRNA and their targets showed that specific miRNAs target the miRNA effector Argonaute 1 (AGO1), drought signaling-related receptors and enzymes, transcription factors, and other structural and functional proteins. The predicted regulatory networks provide insights into a potential miRNA-mediated regulatory mechanism. These results will contribute to the breeding of drought-tolerant tea plants and to elucidating miRNA regulation in response to drought.

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Section: Discussionmentioning

confidence: 99%

Small RNA and degradome profiling reveals important roles for microRNAs and their targets in tea plant response to drought stress

Liu

et al. 2016

Physiologia Plantarum

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“…Ni et al [68] showed that overexpression of miR394a in Arabidopsis reduced the transcript of an F-box gene (At1g27340, also known as LEAF CURLING RESPONSIVENESS, LCR) containing a miR394 complementary target site. In Arabidopsis , a null mutation in DOR gene, which encodes a putative F-box protein, led to a substantial increase in drought tolerance as well as a hypersensitive ABA response of stomatal closing; conversely, the transgenic plants overexpressing DOR gene showed decreased drought tolerance [69].…”

Section: Discussionmentioning

confidence: 99%

Identification of boron-deficiency-responsive microRNAs in Citrus sinensis roots by Illumina sequencing

Yang

et al. 2014

BMC Plant Biol

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BackgroundBoron (B)-deficiency is a widespread problem in many crops, including Citrus. MicroRNAs (miRNAs) play important roles in nutrient deficiencies. However, little is known on B-deficiency-responsive miRNAs in plants. In this study, we first identified miRNAs and their expression pattern in B-deficient Citrus sinensis roots by Illumina sequencing in order to identify miRNAs that might be involved in the tolerance of plants to B-deficiency.ResultsWe isolated 52 (40 known and 12 novel) up-regulated and 82 (72 known and 10 novel) down-regulated miRNAs from B-deficient roots, demonstrating remarkable metabolic flexibility of roots, which might contribute to the tolerance of plants to B-deficiency. A model for the possible roles of miRNAs in the tolerance of roots to B-deficiency was proposed. miRNAs might regulate the adaptations of roots to B-deficiency through following several aspects: (a) inactivating reactive oxygen species (ROS) signaling and scavenging through up-regulating miR474 and down-regulating miR782 and miR843; (b) increasing lateral root number by lowering miR5023 expression and maintaining a certain phenotype favorable for B-deficiency-tolerance by increasing miR394 expression; (c) enhancing cell transport by decreasing the transcripts of miR830, miR5266 and miR3465; (d) improving osmoprotection (miR474) and regulating other metabolic reactions (miR5023 and miR821). Other miRNAs such as miR472 and miR2118 in roots increased in response to B-deficiency, thus decreasing the expression of their target genes, which are involved in disease resistance, and hence, the disease resistance of roots.ConclusionsOur work demonstrates the possible roles of miRNAs and related mechanisms in the response of plant roots to B-deficiency.

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“…Other miRNAs also regulate plant tolerance to drought and salinity stresses. Overexpression of miR394 enhanced plant tolerance to drought in Arabidopsis majorly due to lowered leaf water loss (Ni et al, ) in an ABA‐dependent manner (Song et al, ). However, the same transgenic plants with overexpression of miR 394 show sensitive to salinity (Song et al, ).…”

Section: Mirna‐based Biotechnology For Improving Plant Tolerance To Ementioning

confidence: 99%

MicroRNA‐Based Biotechnology for Plant Improvement

Zhang

Wang

2014

Journal Cellular Physiology

197

123

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MicroRNAs (miRNAs) are an extensive class of newly discovered endogenous small RNAs, which negatively regulate gene expression at the post-transcription levels. As the application of next-generation deep sequencing and advanced bioinformatics, the miRNA-related study has been expended to non-model plant species and the number of identified miRNAs has dramatically increased in the past years. miRNAs play a critical role in almost all biological and metabolic processes, and provide a unique strategy for plant improvement. Here, we first briefly review the discovery, history, and biogenesis of miRNAs, then focus more on the application of miRNAs on plant breeding and the future directions. Increased plant biomass through controlling plant development and phase change has been one achievement for miRNA-based biotechnology; plant tolerance to abiotic and biotic stress was also significantly enhanced by regulating the expression of an individual miRNA. Both endogenous and artificial miRNAs may serve as important tools for plant improvement.

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Overexpression of gma-MIR394a confers tolerance to drought in transgenic Arabidopsis thaliana

Cited by 88 publications

References 30 publications

Small RNA and degradome profiling reveals important roles for microRNAs and their targets in tea plant response to drought stress

Small RNA and degradome profiling reveals important roles for microRNAs and their targets in tea plant response to drought stress

Identification of boron-deficiency-responsive microRNAs in Citrus sinensis roots by Illumina sequencing

MicroRNA‐Based Biotechnology for Plant Improvement

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