Overexpression of glycolysis markers in placental tissue of pregnant women with chronic venous disease: a histological study

Ortega, Miguel A.; Sáez, Miguel A.; Fraile-Martínez, Óscar; Álvarez-Mon, Miguel Ángel; García-Montero, Cielo; Guijarro, Luis G.; Asúnsolo, Ángel; Álvarez‐Mon, Melchor; Buján, Julia; García‐Honduvilla, Natalio; León-Luis, Juan De; Bravo, Coral

doi:10.7150/ijms.65419

Cited by 9 publications

(12 citation statements)

References 50 publications

(83 reference statements)

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“…In this work, we demonstrated increased gene and protein expression of NOX-1, NOX-2, iNOS and PARP, establishing that the placental tissue of FE-PW displayed an augmented expression of OS markers. This can help in understanding the negative consequences of undergoing a psychotic episode during pregnancy, as these placental changes may be indicators of maternofetal suffering [13,37,38].…”

Section: Discussionmentioning

confidence: 99%

“…The median age in the FE-PW group was 33.5 years, and the median gestational age was 40 (38)(39)(40)(41) weeks. For HC-PW, the median age was 33.5 (25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39) years, and the median gestational age was 40 (39)(40)(41)(42) weeks. The sociodemographic and clinical features of these patients are summarized in Table 1.…”

Section: Methodsmentioning

confidence: 99%

“…Five different sections and ten fields were randomly examined for each patient. Immunohistochemical expression was classified as positive(+) when the mean stained area in the studied sample was equal or superior to 5% of the total, following the immunoreactive score (IRS) [37]. This method allowed us to classify immunostaining using the following scale: 0-1, minimum staining (≤25%); 2, moderate staining (25-65%) and 3-4, strong staining (≥65-100%).…”

Section: Evaluation Of Histopathological Expression and Statistical A...mentioning

confidence: 99%

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Evidence of Increased Oxidative Stress in the Placental Tissue of Women Who Suffered an Episode of Psychosis during Pregnancy

et al. 2023

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Psychosis is a complex clinical syndrome resulting in a loss of contact with reality and alterations in behavior and sensorial and motor functions. Although the onset of psychosis can be related to any medical condition, most cases of psychosis are not fully understood. Psychosis may manifest for the first time during pregnancy, which is detrimental to maternofetal well-being. The impact of having a first episode of psychosis during pregnancy on the placenta has not yet been explored. Oxidative stress is thought to take part in the etiopathogenesis of this complex disorder, and this condition can also affect the placenta as it is highly sensitive to changes in the maternal environment. In this sense, the aim of the present work was to study the gene and protein expression through RT–qPCR and immunohistochemistry, respectively, of oxidative stress markers (NOX-1, NOX-2, iNOS, eNOS and PARP) in the placental tissue of women who underwent a first episode of psychosis during pregnancy (FE-PW) in comparison to healthy pregnant women. Our results showed augmented gene and protein expression of NOX-1, NOX-2, iNOS and PARP in the placental tissue of FE-PW. For the first time, we demonstrated that oxidative stress may have an important pathophysiological role in this tissue, aiding in explaining the impact of psychosis on pregnancy and the need for future studies in this field to guide better clinical management of these patients.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Evaluation Of Histopathological Expression and Statistical A...mentioning

confidence: 99%

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Evidence of Increased Oxidative Stress in the Placental Tissue of Women Who Suffered an Episode of Psychosis during Pregnancy

et al. 2023

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“…Preserved MEM samples were washed and rehydrated 5 times in an MEM that was free of antibiotics in order to remove erythrocytes. Each sample was then cut by using another scalpel into 2 cm fragments, later being fixed in F13 (i.e., 60% ethanol, 20% methanol, 7% polyethylene glycol, and 13% distilled water) according to well-established protocols [24]. Thereafter, paraffin-embedded samples were developed using specific molds and once the paraffin was solidified, an HM 350 S rotary microtome (Thermo Fisher Scientific, Inc., Waltham, MA, USA) was used to cut 5 µm thick sections.…”

Section: Sample Collection and Processingmentioning

confidence: 99%

The Placentas of Women Who Suffer an Episode of Psychosis during Pregnancy Have Increased Lipid Peroxidation with Evidence of Ferroptosis

et al. 2023

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Psychosis is a complex entity characterized by psychological, behavioral, and motor alterations resulting in a loss of contact with reality. Although it is not common, pregnancy can be a period in which a first episode of psychosis can manifest, entailing detrimental consequences for both the fetus and the mother. The pathophysiological basis and study of maternofetal wellbeing need to be further elucidated. Lipid peroxidation and ferroptosis are two phenomena that are tightly linked to the placental dysfunction commonly observed in different complications of pregnancy. In the present study, we aim to explore the histopathological and gene expression of different markers of lipid peroxidation and ferroptosis in the placentas of women who underwent a first episode of psychosis during their pregnancy (n = 22). The aim is to then compare them with healthy pregnant women (n = 20). In order to achieve this goal, iron deposits were studied using Prussian Blue staining. In addition, the protein/gene expression of a transferrin receptor (TFRC), as well as an acyl-CoA synthetase long-chain family member 4 (ACSL-4), arachidonate lipoxygenase-5 (ALOX-5), malondialdehyde (MDA), and glutathione peroxidase 4 (GPX4) were all analyzed through gene expression (RT-qPCR) and immunohistochemical procedures. Our results demonstrate an increased presence of iron deposits that are accompanied by a further expression of TFRC, ACSL-4, ALOX-5, MDA, and GPX4—all of which are observed in the placenta tissue of women who have suffered from a first episode of psychosis. Therefore, in our study, a histopathological increase in lipid peroxidation and ferroptosis markers in the affected women is suggested. However, further studies are needed in order to validate our results and to establish possible consequences for the reported alterations.

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“…Both endothelial and vascular smooth muscle cells strongly depend on glucose metabolism and glycolysis in physiological conditions [ 23 , 24 ], and likewise, an aberrant regulation of these processes appears to be critically involved in vascular disorders [ 25 , 26 ]. Histological alterations in glycolysis markers in the placenta of women with CVeD has been recently demonstrated [ 27 ]. In addition, quantitative proteomic analysis has identified a set of glycolytic components significantly altered in VVs of patients with CVeD [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

Venous Wall of Patients with Chronic Venous Disease Exhibits a Glycolytic Phenotype

Fraile-Martínez

García-Montero

Álvarez-Mon

et al. 2022

JPM

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Chronic venous disease (CVeD) is a rising medical condition characterized by a broad spectrum of disorders in the venous system. Varicose veins (VVs) represent a frequent clinical manifestation of CVeD, particularly in the lower limbs. Prior histopathological studies have defined a set of alterations observed in the venous wall of patients with VVs, affecting their structure and behavior. Metabolic changes in the veins appear to be a critical biological mechanism aiding our understanding of the pathogenesis of CVeD. In this sense, previous studies have identified a potential role of a glycolytic phenotype in the development of different vascular disorders; however, its precise role in CVeD remains to be fully explored. Thus, the aim of the present study was to analyze the gene and protein expression of glucose transporter 1 (GLUT-1) and the glycolytic enzymes PGK-1, ALD, GA3PDH and LDH in the VVs of patients with CVeD (n = 35) in comparison to those expressed in healthy subjects. Our results display enhanced gene and protein expression of GLUT-1, PGK-1, ALD, GA3PDH and LDH in patients with CVeD, suggesting a glycolytic switch of the venous tissue. Greater understanding of the impact of this glycolytic switch in patients with CVeD is required to define a possible pathophysiological role or therapeutic implications of these changes.

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Overexpression of glycolysis markers in placental tissue of pregnant women with chronic venous disease: a histological study

Cited by 9 publications

References 50 publications

Evidence of Increased Oxidative Stress in the Placental Tissue of Women Who Suffered an Episode of Psychosis during Pregnancy

Evidence of Increased Oxidative Stress in the Placental Tissue of Women Who Suffered an Episode of Psychosis during Pregnancy

The Placentas of Women Who Suffer an Episode of Psychosis during Pregnancy Have Increased Lipid Peroxidation with Evidence of Ferroptosis

Venous Wall of Patients with Chronic Venous Disease Exhibits a Glycolytic Phenotype

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