Overexpression of glutathione S-transferase/glutathioneperoxidase enhances the growth of transgenic tobacco seedlings during stress

Roxas, Virginia P.; Smith, Roger K.; Allen, Eric R.; Allen, Randy D.

doi:10.1038/nbt1097-988

Cited by 541 publications

(363 citation statements)

References 26 publications

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“…Transgenic tobacco plants overexpressing both GST and GPX biosynthesis genes increased seedling establishment, and seedlings grew faster under salt stress. This effect could be caused by regulation of the glutathione pool (Roxas et al 1997). The antioxidative function of glutathione reduces oxidative stress, prevents lipid peroxidation, and protects the plasma membrane.…”

Section: Glutathione-induced Abiotic Stress Tolerance Salinitymentioning

confidence: 99%

“…Seedling growth in media containing salt is regulated by GSH or the activities of glutathione-dependent and regulating enzymes (Roxas et al 1997;Hussain et al 2008). Transgenic tobacco plants overexpressing both GST and GPX biosynthesis genes increased seedling establishment, and seedlings grew faster under salt stress.…”

Section: Glutathione-induced Abiotic Stress Tolerance Salinitymentioning

confidence: 99%

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Glutathione in plants: biosynthesis and physiological role in environmental stress tolerance

Hasanuzzaman

Nahar

Anee

et al. 2017

Physiol Mol Biol Plants

596

284

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Glutathione (GSH; c-glutamyl-cysteinyl-glycine) is a small intracellular thiol molecule which is considered as a strong non-enzymatic antioxidant. Glutathione regulates multiple metabolic functions; for example, it protects membranes by maintaining the reduced state of both a-tocopherol and zeaxanthin, it prevents the oxidative denaturation of proteins under stress conditions by protecting their thiol groups, and it serves as a substrate for both glutathione peroxidase and glutathione S-transferase. By acting as a precursor of phytochelatins, GSH helps in the chelating of toxic metals/metalloids which are then transported and sequestered in the vacuole. The glyoxalase pathway (consisting of glyoxalase I and glyoxalase II enzymes) for detoxification of methylglyoxal, a cytotoxic molecule, also requires GSH in the first reaction step. For these reasons, much attention has recently been directed to elucidation of the role of this molecule in conferring tolerance to abiotic stress. Recently, this molecule has drawn much attention because of its interaction with other signaling molecules and phytohormones. In this review, we have discussed the recent progress in GSH biosynthesis, metabolism and its role in abiotic stress tolerance.

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Section: Glutathione-induced Abiotic Stress Tolerance Salinitymentioning

confidence: 99%

Section: Glutathione-induced Abiotic Stress Tolerance Salinitymentioning

confidence: 99%

Glutathione in plants: biosynthesis and physiological role in environmental stress tolerance

Hasanuzzaman

Nahar

Anee

et al. 2017

Physiol Mol Biol Plants

596

284

View full text Add to dashboard Cite

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“…In addition, dehydrins, which play a role in the response to cold/drought 44 , had only two members in G. montanum, compared with 38 in P. abies, 28 in P. taeda and 3-15 in angiosperms (Supplementary Table 19). Further analysis of the G. montanum genome also revealed relatively few gene family members of the AP2 domain containing protein families, which are involved in the cold stress response 45,46 , and glutathione peroxidase and glutathione S-transferase families, involved in the oxidant stress response 47,48 . Taken together, these data appear consistent with the hypothesis that the ecological shift to a warm, wet forest habitat is associated with a relaxation of selection pressure on genes associated with water stress and low temperature.…”

mentioning

confidence: 99%

A genome for gnetophytes and early evolution of seed plants

et al. 2018

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Gnetophytes are an enigmatic gymnosperm lineage comprising three genera, Gnetum, Welwitschia and Ephedra, which are morphologically distinct from all other seed plants. Their distinctiveness has triggered much debate as to their origin, evolution and phylogenetic placement among seed plants. To increase our understanding of the evolution of gnetophytes, and their relation to other seed plants, we report here a high-quality draft genome sequence for Gnetum montanum, the first for any gnetophyte. By using a novel genome assembly strategy to deal with high levels of heterozygosity, we assembled >4 Gb of sequence encoding 27,491 protein-coding genes. Comparative analysis of the G. montanum genome with other gymnosperm genomes unveiled some remarkable and distinctive genomic features, such as a diverse assemblage of retrotransposons with evidence for elevated frequencies of elimination rather than accumulation, considerable differences in intron architecture, including both length distribution and proportions of (retro) transposon elements, and distinctive patterns of proliferation of functional protein domains. Furthermore, a few gene families showed Gnetum-specific copy number expansions (for example, cellulose synthase) or contractions (for example, Late Embryogenesis Abundant protein), which could be connected with Gnetum's distinctive morphological innovations associated with their adaptation to warm, mesic environments. Overall, the G. montanum genome enables a better resolution of ancestral genomic features within seed plants, and the identification of genomic characters that distinguish Gnetum from other gymnosperms. NATuRe PLANTS ArticlesNATurE PLANTs phylogenetic position of gnetophytes, with topologies differing depending on the type of sequence data (for example, plastid versus nuclear genes, nucleotide versus amino acid data) and analytical approach used (for example, maximum parsimony, maximum likelihood, Bayesian, multispecies coalescent based methods) [6][7][8] . Consequently, several possible hypotheses have been put forward that place gnetophytes as sister to (1) Pinaceae ('Gnepine' hypothesis); (2) cupressophytes ('Gnecup' hypothesis); (3) all conifers ('Gnetifer' hypothesis); (4) all other gymnosperms; or (5) all seed plants 9 . Currently, the emerging consensus, based on both older and more recent studies, and recently released data from the 1KP initiative (see https://sites.google.com/a/ualberta.ca/onekp/, and Wickett et al. 8 ), indicates that gnetophytes are sister to, or within, the conifers.So far, the availability of whole genome sequences for gymnosperms has been limited to conifers (specifically to Pinaceae) [10][11][12][13] and G. biloba 14 , with no whole genome assemblies available for the two remaining major seed plant lineages-cycads and gnetophytes. This deficiency, together with the conflicting phylogenetic evidence for relationships among these groups, is impeding our understanding of genome evolution across all seed plants. Here, we present a high-quality draft genome of Gnetum ...

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“…Lipid hydroperoxide contents were determined by measuring MDA using the 2-thiobarbituric acid assay as described previously (Roxas et al, 1997). MDA was estimated by measuring A 535 and using a molar absorption coefficient of 1.56 3 10 5 mM 21 cm 21 (Gueta-Dahan et al, 1997).…”

Section: Analysis Of Lipid Peroxidationmentioning

confidence: 99%

Induction and Functional Analysis of Two Reduced Nicotinamide Adenine Dinucleotide Phosphate-Dependent Glutathione Peroxidase-Like Proteins in Synechocystis PCC 6803 during the Progression of Oxidative Stress

et al. 2004

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Synechocystis PCC 6803 contains two types of glutathione peroxidase-like proteins (GPX-1 and GPX-2) that utilize NADPH but not reduced glutathione and unsaturated fatty acid hydroperoxides or alkyl hydroperoxides. The steady-state transcript level of gpx-1 gradually increased under oxidative stress conditions imposed by high light intensity, high salinity, or application of methylviologen or t-butyl hydroperoxide in the wild-type and GPX-2 knock-out mutant (gpx-2D) cells. To examine the ability of GPX-1, GPX-2, and thioredoxin peroxidase to scavenge lipid hydroperoxide in vivo, we measured the photosynthetic evolution of O 2 and the level of lipid peroxidation in the wild-type and each type of mutant cell after the application of t-butyl hydroperoxide or H 2 O 2 . The data reported here indicate that GPX-1 and GPX-2 are essential for the removal of lipid hydroperoxides under normal and stress conditions, leading to the protection of membrane integrity.

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Overexpression of glutathione S-transferase/glutathioneperoxidase enhances the growth of transgenic tobacco seedlings during stress

Cited by 541 publications

References 26 publications

Glutathione in plants: biosynthesis and physiological role in environmental stress tolerance

Glutathione in plants: biosynthesis and physiological role in environmental stress tolerance

A genome for gnetophytes and early evolution of seed plants

Induction and Functional Analysis of Two Reduced Nicotinamide Adenine Dinucleotide Phosphate-Dependent Glutathione Peroxidase-Like Proteins in Synechocystis PCC 6803 during the Progression of Oxidative Stress

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