Overexpression of four Arabidopsis thaliana NHL genes in soybean (Glycine max) roots and their effect on resistance to the soybean cyst nematode (Heterodera glycines)

Maldonado, Andrea; Youssef, Reham M.; McDonald, Margaret; Brewer, Eric; Beard, Hunter S.; Matthews, Benjamin F.

doi:10.1016/j.pmpp.2014.02.001

Cited by 11 publications

(12 citation statements)

References 35 publications

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“…2a), suggesting that CaNHLs might participate in the pepper defense response. Many studies have shown that NHLs participate in the JA-mediated and ET-mediated defense response pathways 27 . In pepper, CaNHL9 and CaNHL10 contain 10 MeJA-responsive elements, CaNHL4 has six MeJA-responsive elements, and the remaining genes, including CaNHL11 and CaNHL15, also have MeJAresponsive elements (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…It has been documented that members of the NHL family play an important role in plant disease resistance 9 . Maldonado et al found that overexpression of GmNHL1 and GmNHL8 in A. thaliana plants enhanced resistance to Heterodera glycines by activating the jasmonic acid (JA) and ethylene (ET) pathways, suggesting that NHL1 and NHL8 contribute to the plant defense response against pathogens 11 . Chen et al reported that overexpression of StPOTHR1, a member of the NHL gene family, enhances resistance against Phytophthora infestans by restricting rapid pathogen proliferation 12 .…”

Section: Introductionmentioning

confidence: 99%

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Genome-wide analysis of NDR1/HIN1-like genes in pepper (Capsicum annuum L.) and functional characterization of CaNHL4 under biotic and abiotic stresses

Liu

Peng

et al. 2020

Hortic Res

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Plant NDR1/HIN1-like (NHL) genes play an important role in triggering plant defenses in response to biotic stresses. In this study, we performed a genome-wide identification of the NHL genes in pepper (Capsicum annuum L.) and characterized the functional roles of these CaNHL genes in response to abiotic stresses and infection by different pathogens. Phylogenetic analysis revealed that CaNHLs can be classified into five distinct subgroups, with each group containing generic and specific motifs. Regulatory element analysis showed that the majority of the promoter regions of the identified CaNHLs contain jasmonic acid (JA)-responsive and salicylic acid (SA)-responsive elements, and transcriptomic analysis revealed that CaNHL genes are expressed in all the examined tissues of pepper. The CaNHL1, CaNHL4, CaNHL6, CaNHL10, CaNHL11, and CaNHL12 genes were significantly upregulated under abiotic stress as well as in response to different pathogens, such as TMV, Phytophthora capsici and Pseudomonas syringae. In addition, we found that CaNHL4 localizes to the plasma membrane. CaNHL4-silenced pepper plants display significantly increased susceptibility to TMV, Phytophthora capsici and Pseudomonas syringae, exhibiting reduced expression of JA-related and SA-related genes and reduced ROS production. However, transient overexpression of CaNHL4 in pepper increases the expression of JArelated and SA-related genes, enhances the accumulation of ROS, and inhibits the infection of these three pathogens. Collectively, for the first time, we identified the NHL genes in pepper and demonstrated that CaNHL4 is involved in the production of ROS and that it also regulates the expression of JA-related and SA-related genes in response to different pathogens, suggesting that members of the CaNHL family play an essential role in the disease resistance of pepper.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis of NDR1/HIN1-like genes in pepper (Capsicum annuum L.) and functional characterization of CaNHL4 under biotic and abiotic stresses

Liu

Peng

et al. 2020

Hortic Res

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“…Our understanding is based on the functioning of the orthologous HIN1 in Nicotiana tabacum and non-race-specific disease resistance 1 (NDR1) in A. thaliana. Both genes were reported to be involved in the activation of hypersensitive resistance and interaction of CC-NB-LRR R proteins (Dörmann et al, 2000;Maldonado et al, 2014). Moreover, the gene expression of a GSK3/SHAGGY-like protein kinase was also inhibited in both periods (Fig.…”

Section: Quantitative Gene Expression Analysis Using Real-time Pcrmentioning

confidence: 90%

Analysis of plant gene expression during passion fruit–Xanthomonas axonopodisinteraction implicates lipoxygenase 2 in host defence

Munhoz

Santos

Arenhart

et al. 2015

Annals of Applied Biology

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Passiflora edulis is the major species of passionflowers grown worldwide, mainly for juice production and fresh fruit, in climates ranging from cool subtropical (purple variety) to warm tropical (yellow variety). The bacterial leaf spot, caused by Xanthomonas axonopodis pv. passiflorae (Xap) can be a serious disease affecting passion fruit production in commercial orchards, particularly under moist field conditions. In this study we describe a first analysis of host gene expression in this pathosystem. We used suppression subtractive hybridization to construct two cDNA libraries enriched for transcripts induced and repressed by Xap, respectively, 24 h post inoculation with a highly virulent strain. High-quality sequences were obtained resulting in 998 unisequences that were used for annotation. In accordance with BLASTX results performed by Blast2GO tool, 86.7% of the unisequences showed similarity to other plant species' proteins related to different functional categories. Sixty-three transcripts were similar to Arabidopsis thaliana defence-related proteins identified in the PLAZA platform. In silico predicted protein-protein interactions were detected on the basis of the STRING database for 35 of the 63 defence-related proteins. At this early stage of interaction, a set of genes was selected from Blast2GO categorization results and analysed by quantitative PCR (qPCR). The expression profiles changed in response to the pathogen for 76% of these genes (48/63) and the differences in expression ratios ranged from 0.51-fold to 1.83-fold. In later stages of interactions (5 and 9 days post inoculation) when disease-associated symptoms were visible, qPCR analyses were performed for 14 genes selected from both libraries. The expression profiles of all genes were found to be changed by the pathogen. The gene that responded most strongly to the pathogen attack encodes a lipoxygenase 2. In inoculated plants, its expression was induced 500-fold and 300-fold, 5 and 9 dpi, respectively, compared to controls, suggesting an important role of this gene in passion fruit defence. Moreover, we showed that most of the genes involved in well-known pathogen recognition signalling pathways were repressed by Xap and this lends support to the idea that the jasmonic acid signalling pathway fails to be activated during the first hours of interaction.

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“…Field trial of Xanthomonas wilt disease-resistant bananas have been carried out using vegetatively propagated bananas [ 25 ]. In other studies, vegetatively micro-propagated plants have been used to confirm the role of A. thaliana NHL1 and NHL8 genes in the Soybean defence mechanism against Heterodera glycines [ 26 ] and in post-transcriptional hairpin RNA-mediated gene silencing of vital fungal genes to confer resistance against Fusarium wilt in banana [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

The ZmRCP-1 promoter of maize provides root tip specific expression of transgenes in plantain

Onyango

Roderick

Tripathi

et al. 2016

J of Biol Res-Thessaloniki

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BackgroundBananas and plantains (Musa spp.) provide 25 % of the food energy requirements for more than 100 million people in Africa. Plant parasitic nematodes cause severe losses to the crop due to lack of control options. The sterile nature of Musa spp. hampers conventional breeding but makes the crop suitable for genetic engineering. A constitutively expressed synthetic peptide in transgenic plantain has provided resistance against nematodes. Previous work with the peptide in potato plants indicates that targeting expression to the root tip improves the efficacy of the defence mechanism. However, a promoter that will provide root tip specific expression of transgenes in a monocot plant, such as plantain, is not currently available. Here, we report the cloning and evaluation of the maize root cap-specific protein-1 (ZmRCP-1) promoter for root tip targeted expression of transgenes that provide a defence against plant parasitic nematodes in transgenic plantain.ResultsOur findings indicate that the maize ZmRCP-1 promoter delivers expression of β-glucuronidase (gusA) gene in roots but not in leaves of transgenic plantains. In mature old roots, expression of gusA gene driven by ZmRCP-1 becomes limited to the root cap. Invasion by the nematode Radopholus similis does not modify Root Cap-specific Protein-1 promoter activity.ConclusionsRoot cap-specific protein-1 promoter from maize can provide targeted expression of transgene for nematode resistance in transgenic plantain.

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Overexpression of four Arabidopsis thaliana NHL genes in soybean (Glycine max) roots and their effect on resistance to the soybean cyst nematode (Heterodera glycines)

Cited by 11 publications

References 35 publications

Genome-wide analysis of NDR1/HIN1-like genes in pepper (Capsicum annuum L.) and functional characterization of CaNHL4 under biotic and abiotic stresses

Genome-wide analysis of NDR1/HIN1-like genes in pepper (Capsicum annuum L.) and functional characterization of CaNHL4 under biotic and abiotic stresses

Analysis of plant gene expression during passion fruit–Xanthomonas axonopodisinteraction implicates lipoxygenase 2 in host defence

The ZmRCP-1 promoter of maize provides root tip specific expression of transgenes in plantain

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