Overexpression of endogenous delta-6 fatty acid desaturase gene enhances eicosapentaenoic acid accumulation in Phaeodactylum tricornutum

Zhu, Bo; Tu, Changchao; Shi, Hong-Ping; Yang, Guanpin; Pan, Kehou

doi:10.1016/j.procbio.2017.03.013

Cited by 36 publications

(14 citation statements)

References 44 publications

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“…Interestingly, total lipid productivity was remarkably increased in transgenic cells and reached up to 21 mg/gDCW/day ( Figure 2F). Delta-6 fatty acid desaturase overexpression significantly elevated total lipid in P. tricornutum (Zhu et al, 2017). Elongase overexpression increased TAG in Thalassiosira pseudonana (Cook and Hildebrand, 2015).…”

Section: Lipidomic Studies Demonstrated That Nod6 Elevated Total Lipimentioning

confidence: 94%

“…Given the crucial role of 6-desaturase in catalyzing the first committed step of generating EPA from ALA, it emerged as the potential metabolic target for LC-PUFA overproduction (Zhu et al, 2017). Various reports demonstrated the exploitation of elongases, desaturases, fatty acid synthase or combinatorial multiple gene expression to hyperaccumulate algal LC-PUFAs (Martins et al, 2013;Gong et al, 2014), however, promising strategies and molecular candidates are yet to be characterized (Domergue et al, 2002).…”

Section: Nod6 Overexpression Significantly Elevated Lc-pufa In N Ocementioning

confidence: 99%

“…Various reports demonstrated the exploitation of elongases, desaturases, fatty acid synthase or combinatorial multiple gene expression to hyperaccumulate algal LC-PUFAs (Martins et al, 2013;Gong et al, 2014), however, promising strategies and molecular candidates are yet to be characterized (Domergue et al, 2002). Zhu et al (2017) reported the overexpression of 6-desaturase enhanced EPA up to 38.101 mg/g DW in P. tricornutum (Zhu et al, 2017). Expression of multiple desaturase genes increased EPA by 25% in Nannochloropsis oceanica (Poliner et al, 2018).…”

Section: Nod6 Overexpression Significantly Elevated Lc-pufa In N Ocementioning

confidence: 99%

“…Thereafter, the unsaturation of LA to produce γ-linolenic acid (GLA) is catalyzed by 6-desaturase, which could be further elongated to produce LC-PUFA. Despite the involvement of various key proteins, 6-desaturase of the ω-3 pathway has been considered as a unique rate-limiting enzyme in EPA biosynthesis that present in algae but not in plants (Zhu et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Harnessing the Lipogenic Potential of Δ6-Desaturase for Simultaneous Hyperaccumulation of Lipids and Polyunsaturated Fatty Acids in Nannochloropsis oceanica

Yang

Yuan

et al. 2019

Front. Mar. Sci.

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Burgeoning demand for long-chain polyunsaturated fatty acids (LC-PUFA) due to their established pharmacological and economic significances along with the declining sources of fish urge to explore the sustainable sources of LC-PUFA. Being the predominant LC-PUFA source for marine fishes at the base of the aquatic food web, microalgae has been hailed as a promising natural source for LC-PUFA. However, the potential of algal systems to overproduce LC-PUFA via metabolic engineering is warranted to meet the ever-increasing demand. In this study, we identified and overexpressed 6-desaturase, the key enzyme involved in fatty acid desaturation and exemplified its potential on elevating PUFA and lipid content in Nannochloropsis oceanica. 6-desaturase overexpression enhanced growth and photosynthetic efficiency. Transgenic cells exhibited a remarkable increase in EPA content and reached up to 62.35 mg/g DW, the highest EPA production in transgenic N. oceanica by expressing a single key enzyme without impeding growth. Total lipid content was significantly increased by 1.7-fold in the transgenic cell than WT. Together, these findings exemplify a potential candidate for LC-PUFA overproduction and also open a new avenue for sustainable production of microalgal PUFAs.

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Section: Lipidomic Studies Demonstrated That Nod6 Elevated Total Lipimentioning

confidence: 94%

Section: Nod6 Overexpression Significantly Elevated Lc-pufa In N Ocementioning

confidence: 99%

Section: Nod6 Overexpression Significantly Elevated Lc-pufa In N Ocementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Harnessing the Lipogenic Potential of Δ6-Desaturase for Simultaneous Hyperaccumulation of Lipids and Polyunsaturated Fatty Acids in Nannochloropsis oceanica

Yang

Yuan

et al. 2019

Front. Mar. Sci.

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“…-linolenic acid (GLA, 18:3 6, 9,12 ) and stearidonic acid (SDA, 18:4 6, 9,12,15 ), respectively (2)(3)(4). The level of FADS2 catalytic activity determines the rate at which LA and ALA are converted as well as the volume and type of products produced.…”

mentioning

confidence: 99%

Molecular mechanisms underlying catalytic activity of delta 6 desaturase from Glossomastix chrysoplasta and Thalassiosira pseudonana

Shi

粟

et al. 2018

Journal of Lipid Research

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Delta 6 desaturase (FADS2) is a critical bifunctional enzyme required for PUFA biosynthesis. In some organisms, FADS2s have high substrate specificity, whereas in others, they have high catalytic activity. Previously, we analyzed the molecular mechanisms underlying high FADS2 substrate specificity; in this study, we assessed those underlying the high catalytic activity of FADS2s from and To understand the structural basis of this catalytic activity, GcFADS2 and TpFADS2 sequences were divided into nine sections, and a domain-swapping approach was applied to examine the role of each section in facilitating the catalytic activity of the overall protein. The results revealed two regions essential to this process: one that extends from the end of the fourth to the beginning of the fifth cytoplasmic transmembrane domain, and another that includes the C-terminal region that occurs after the sixth cytoplasmic transmembrane domain. Based on the domain-swapping analyses, the amino acid residues at ten sites were identified to differ between the GcFADS2 and TpFADS2 sequences, and therefore further analyzed by site-directed mutagenesis. T302V, S322A, Y375F, and M384S/M385 substitutions in TpFADS2 significantly affected FADS2 catalytic efficiency. This study offers a solid basis for in-depth understanding of catalytic efficiency of FADS2.

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The Role of Metabolic Engineering for Enhancing PUFA Production in Microalgae

Arora

2020

Nutraceutical Fatty Acids From Oleaginous Microalgae

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Overexpression of endogenous delta-6 fatty acid desaturase gene enhances eicosapentaenoic acid accumulation in Phaeodactylum tricornutum

Cited by 36 publications

References 44 publications

Harnessing the Lipogenic Potential of Δ6-Desaturase for Simultaneous Hyperaccumulation of Lipids and Polyunsaturated Fatty Acids in Nannochloropsis oceanica

Harnessing the Lipogenic Potential of Δ6-Desaturase for Simultaneous Hyperaccumulation of Lipids and Polyunsaturated Fatty Acids in Nannochloropsis oceanica

Molecular mechanisms underlying catalytic activity of delta 6 desaturase from Glossomastix chrysoplasta and Thalassiosira pseudonana

The Role of Metabolic Engineering for Enhancing PUFA Production in Microalgae

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