Overexpression of Efflux Pumps Mediate Pan Resistance of <i>Klebsiella pneumoniae</i> Sequence Type 11

Lv, Fei; Cai, Jingjing; He, Qixia; Wang, Weiqi; Luo, Yi; Wang, Xin; Mi, Na; Zhao, Zuguo; Li, Guoming; Luo, Wenxin

doi:10.1089/mdr.2020.0395

Cited by 22 publications

(17 citation statements)

References 42 publications

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“…The deleterious effect of detected mutations on the function of proteins was investigated using Protein Variation Effect Analyzer (PROVEAN; http://provean.jcvi.org/index.php). Moreover, we investigated the frequency of the mutations found in PmrA/B, CrrA/B [20] and AcrR/S [21][22][23] in all ST307 among the 6,412 genomes of K. pneumoniae available on NCBI (last upgrade April 2019) by Basic Local Alignment Search Tool (BLAST) protein (blastp) using as threshold expect (E)-value 10e −5 . Next we performed MLST using PubMLST typing schemes by local BLAST on all K. pneumoniae genomes available in NCBI to compare the frequency of different STs in this database.…”

Section: In Silico Mutations Analysismentioning

confidence: 99%

Risk factors for acquisition of colistin-resistant Klebsiella pneumoniae and expansion of a colistin-resistant ST307 epidemic clone in hospitals in Marseille, France, 2014 to 2017

et al. 2021

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Background France is a low prevalence country for colistin resistance. Molecular and epidemiological events contributing to the emergence of resistance to colistin, one of the 'last-resort' antibiotics to treat multidrug-resistant Gram-negative infections, are important to investigate. Aim This retrospective (2014 to 2017) observational study aimed to identify risk factors associated with acquisition of colistin-resistant Klebsiella pneumoniae (CRKP) in hospitals in Marseille, France, and to molecularly characterise clinical isolates. Methods To identify risk factors for CRKP, a matched-case–control (1:2) study was performed in two groups of patients with CRKP or colistin-susceptible K. pneumoniae respectively. Whole-genome-sequences (WGS) of CRKP were compared with 6,412 K. pneumoniae genomes available at the National Center for Biotechnology Information (NCBI). Results Multivariate analysis identified male sex and contact with a patient carrying a CRKP as significant independent factors (p < 0.05) for CRKP acquisition, but not colistin administration. WGS of nine of 14 CRKP clinical isolates belonged to the same sequence type (ST)307. These isolates were from patients who had been hospitalised in the same wards, suggesting an outbreak. Comparison of the corresponding strains’ WGS to K. pneumoniae genomes in NCBI revealed that in chromosomal genes likely playing a role in colistin resistance, a subset of five specific mutations were significantly associated with ST307 (p < 0.001). Conclusion A ST307 CRKP clone was identified in this study, with specific chromosomal mutations in genes potentially implicated in colistin resistance. ST307 might have a propensity to be or become resistant to colistin, however confirming this requires further investigations.

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Section: In Silico Mutations Analysismentioning

confidence: 99%

Risk factors for acquisition of colistin-resistant Klebsiella pneumoniae and expansion of a colistin-resistant ST307 epidemic clone in hospitals in Marseille, France, 2014 to 2017

et al. 2021

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“…The MICs of chloramphenicol decreased by 2-fold in Δ kbvR + ompK36 compared to those in WT. This finding may be attributed to the downregulation of KbvR on the expression of AcrAB-TolC efflux pump, which influences the susceptibility to chloramphenicol with the outer membrane porins by decreasing the drug efflux from the cytoplasm ( 30 – 32 ).…”

Section: Resultsmentioning

confidence: 99%

High Osmotic Stress Increases OmpK36 Expression through the Regulation of KbvR to Decrease the Antimicrobial Resistance of Klebsiella pneumoniae

Wang

Tian

et al. 2022

Microbiol Spectr

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“…As rates of antibiotic-resistant bacterial infections continue to rise precipitously, phage therapy research has regained its popularity [11][12][13][14][15]. Antibiotic resistance is a growing problem, with many multi-drug resistant (MDR) bacteria emerging every year, including the appearance of so called "pan-resistant" strains, which are resistant to all or nearly all antibiotics on the market [16][17][18][19]. A major downside of antibiotics when compared to phages is their limited options.…”

Section: Broader Applications Of the Viruses Of Bacteriamentioning

confidence: 99%

Flagellotropic Bacteriophages: Opportunities and Challenges for Antimicrobial Applications

Esteves

Scharf

2022

IJMS

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Bacteriophages (phages) are the most abundant biological entities in the biosphere. As viruses that solely infect bacteria, phages have myriad healthcare and agricultural applications including phage therapy and antibacterial treatments in the foodservice industry. Phage therapy has been explored since the turn of the twentieth century but was no longer prioritized following the invention of antibiotics. As we approach a post-antibiotic society, phage therapy research has experienced a significant resurgence for the use of phages against antibiotic-resistant bacteria, a growing concern in modern medicine. Phages are extraordinarily diverse, as are their host receptor targets. Flagellotropic (flagellum-dependent) phages begin their infection cycle by attaching to the flagellum of their motile host, although the later stages of the infection process of most of these phages remain elusive. Flagella are helical appendages required for swimming and swarming motility and are also of great importance for virulence in many pathogenic bacteria of clinical relevance. Not only is bacterial motility itself frequently important for virulence, as it allows pathogenic bacteria to move toward their host and find nutrients more effectively, but flagella can also serve additional functions including mediating bacterial adhesion to surfaces. Flagella are also a potent antigen recognized by the human immune system. Phages utilizing the flagellum for infections are of particular interest due to the unique evolutionary tradeoff they force upon their hosts: by downregulating or abolishing motility to escape infection by a flagellotropic phage, a pathogenic bacterium would also likely attenuate its virulence. This factor may lead to flagellotropic phages becoming especially potent antibacterial agents. This review outlines past, present, and future research of flagellotropic phages, including their molecular mechanisms of infection and potential future applications.

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Overexpression of Efflux Pumps Mediate Pan Resistance of Klebsiella pneumoniae Sequence Type 11

Cited by 22 publications

References 42 publications

Risk factors for acquisition of colistin-resistant Klebsiella pneumoniae and expansion of a colistin-resistant ST307 epidemic clone in hospitals in Marseille, France, 2014 to 2017

Risk factors for acquisition of colistin-resistant Klebsiella pneumoniae and expansion of a colistin-resistant ST307 epidemic clone in hospitals in Marseille, France, 2014 to 2017

High Osmotic Stress Increases OmpK36 Expression through the Regulation of KbvR to Decrease the Antimicrobial Resistance of Klebsiella pneumoniae

Flagellotropic Bacteriophages: Opportunities and Challenges for Antimicrobial Applications

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