Overexpression of Derlin 3 is associated with malignant phenotype of breast cancer cells

Shibata, Masahiro; Kanda, Mitsuro; Tanaka, Haruyoshi; Umeda, Shinichi; Miwa, Takashi; Shimizu, Dai; Hayashi, Masamichi; Inaishi, Takahiro; Miyajima, Nobuyuki; Adachi, Yayoi; Takano, Yuko; Nakanishi, Kenichi; Takeuchi, Daiji; Noda, Seiya; Kodera, Yasuhiro; Kikumori, Toyone

doi:10.3892/or.2017.5800

Cited by 25 publications

(38 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most recently, increased transcripts of the critical ERAD-associated ubiquitin ligase derlin 3 ( DERL3 ) have been reported in metastatic patient-derived breast cancer samples, correlating with poor disease prognosis [ 50 ]. Inhibition of DERL3 expression substantially abrogated proliferation and invasiveness in breast cancer cell lines, while pharmacological impairment of two other steps in ERAD by the anti-cancer agents Eeyarestatin I or Bortezomib-promoted cell death in the JeKo-1 mantle cell lymphoma cell line [ 50 , 51 ]. From these, it could be argued that precluding the clearance of aberrantly misfolded proteins induces apoptosis by increasing the proteotoxic load beyond manageable levels.…”

Section: Cellular Stress En Route To Tumourigenesis: the Ld Connectiomentioning

confidence: 99%

Dropping in on lipid droplets: insights into cellular stress and cancer

et al. 2018

View full text Add to dashboard Cite

Lipid droplets (LD) have increasingly become a major topic of research in recent years following its establishment as a highly dynamic organelle. Contrary to the initial view of LDs being passive cytoplasmic structures for lipid storage, studies have provided support on how they act in concert with different organelles to exert functions in various cellular processes. Although lipid dysregulation resulting from aberrant LD homeostasis has been well characterised, how this translates and contributes to cancer progression is poorly understood. This review summarises the different paradigms on how LDs function in the regulation of cellular stress as a contributing factor to cancer progression. Mechanisms employed by a broad range of cancer cell types in differentially utilising LDs for tumourigenesis will also be highlighted. Finally, we discuss the potential of targeting LDs in the context of cancer therapeutics.

show abstract

Section: Cellular Stress En Route To Tumourigenesis: the Ld Connectiomentioning

confidence: 99%

Dropping in on lipid droplets: insights into cellular stress and cancer

et al. 2018

View full text Add to dashboard Cite

show abstract

“…The proliferation of MKN1 and N87 cells was evaluated using the Premix WST-8 Cell Proliferation assay Kit (DOJINDO Inc.; ref. 23). The invasion of a Matrigel matrix by MKN1 cells was determined using BioCoat Matrigel invasion chambers (BD Biosciences).…”

Section: Cell Proliferation Migration Invasion Migration and Adhementioning

confidence: 99%

Pattern-Specific Transcriptomics Identifies ASGR2 as a Predictor of Hematogenous Recurrence of Gastric Cancer

Tanaka

Kanda

Miwa

et al. 2018

Molecular Cancer Research

Self Cite

View full text Add to dashboard Cite

Hematogenous recurrence is a challenging clinical finding that often leads to fatalities of patients with gastric cancer. Therefore, the identification of specific biomarkers and potential therapeutic target molecules for hematogenous recurrence is required to improve the outcomes of these patients. Here, transcriptome and bioinformatics analyses were conducted to uncover candidate molecules differentially expressed in patients with hematogenous recurrence of gastric cancer. One potential candidate identified was asialoglycoprotein receptor 2 (), and siRNA experiments were conducted to determine the effect of manipulating expression has on cell phenotypes. mRNA expression analysis using quantitative real-time reverse-transcription PCR was conducted with stage II/III gastric cancer clinical specimens ( = 95). Transcript levels were increased in gastric cancer cells as compared with a control nontumorigenic epithelial cell line. Knockdown of decreased the adhesion and migration potential. Thus, although gastric cancer cell-invasive activity was significantly decreased by knockdown, forced expression of promoted invasive activity. Using a mouse hepatic metastasis model, knockdown of resulted in the absence of hepatic metastasis formation. High expression in primary gastric cancer tissues was an independent predictor of shorter disease-free and overall survival. Finally, patients with high expression were more likely to have a high cumulative rate of hematogenous recurrence but not peritoneal or nodal recurrence. expression is associated with the malignant phenotypes in gastric cancer and represents a specific biomarker of hematogenous recurrences after curative resection for gastric cancer. .

show abstract

“…The resected BC specimens were diagnosed histologically as BC and classified using the Union for International Cancer Control (UICC) staging system for BC (7th edition). Administration of adjuvant medication therapy was determined by physician discretion considering each patient's general condition, pathological feature and subtype (16,17).…”

Section: Sample Collectionmentioning

confidence: 99%

“…RNA was extracted from cell lines (8.0x10 6 cells per cell line), and BC and non-cancerous specimens from 167 patients. cDNA was synthesized as previously described (16,17). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA levels were evaluated for normalizing RASEF mRNA expression levels.…”

Section: Reverse Transcription-quantitative Polymerase Chain Reactionmentioning

confidence: 99%

RASEF expression correlates with hormone receptor status in breast cancer

et al. 2018

Self Cite

View full text Add to dashboard Cite

Breast cancer (BC) is the most frequently diagnosed malignant tumor in women worldwide, and the development of new molecules associated with BC is essential for the management of this disease. RAS and EF-hand domain-containing (RASEF) encodes the GTPase enzyme that belongs to the Rab family. Although the effects of this gene have been reported in several malignant tumor types, the role of RASEF in BC has not been completely elucidated. The aim of the present study was to investigate the importance of RASEF expression in BC. RASEF mRNA expression levels were evaluated in BC and non-cancerous mammary cell lines. The association between RASEF mRNA expression levels and clinicopathological factors in 167 patients with BC were then determined. Among the 13 examined BC cell lines, ER-negative/HER2-negative cell lines expressed lower RASEF mRNA levels, when compared with the other examined cell lines (P=0.014). Of the 167 patients examined, patients with negative hormone receptor status exhibited significantly lower RASEF mRNA expression levels (P<0.001). In addition low RASEF expression in BC tissues was associated with negative estrogen receptor status (P<0.001), negative progesterone receptor status (P<0.001), and triple-negative status (P<0.001). Additionally, although the differences were not statistically significant, patients with low RASEF expression levels exhibited poorer disease-free survival (P=0.123) and overall survival (P=0.086) than other patients. The results of the present study indicate that RASEF mRNA expression levels are associated with hormone receptor status in BC.

show abstract

Overexpression of Derlin 3 is associated with malignant phenotype of breast cancer cells

Cited by 25 publications

References 31 publications

Dropping in on lipid droplets: insights into cellular stress and cancer

Dropping in on lipid droplets: insights into cellular stress and cancer

Pattern-Specific Transcriptomics Identifies ASGR2 as a Predictor of Hematogenous Recurrence of Gastric Cancer

RASEF expression correlates with hormone receptor status in breast cancer

Contact Info

Product

Resources

About