Overexpression of delta-12 desaturase in the yeast Schwanniomyces occidentalis enhances the production of linoleic acid

Lamers, Dennis; Visscher, Bram; Weusthuis, Ruud A.; Francke, Christof; Wijffels, René H.; Lokman, Christien

doi:10.1016/j.biortech.2019.121672

Cited by 17 publications

(17 citation statements)

References 51 publications

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“…These 3 bacteria were higher in the microplastic treatments than in the control treatments (Hao et al 2017). Mortierella could produce high levels of polyunsaturated fatty acids, which had strong antioxidant and antibacterial activities and could reduce environmental stresses (Du et al 2019; Lamers et al 2019). In addition, bacteria belonging to the phylum Actinobacteria in the guts of earthworms have been found to have the capacity to degrade LDPE (Lwanga et al 2018).…”

Section: Discussionmentioning

confidence: 99%

Microplastic Addition Alters the Microbial Community Structure and Stimulates Soil Carbon Dioxide Emissions in Vegetable‐Growing Soil

Gao

Yao

et al. 2020

Enviro Toxic and Chemistry

230

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Microplastic pollution has become an increasingly pervasive issue worldwide, but little is known about its effects on the soil environment. A soil microcosm experiment was conducted using low‐density polyethylene microplastics to estimate the effect of microplastic pollution on soil nutrient cycling and the soil microbial community structure. The results showed that microplastic addition significantly promoted soil carbon dioxide emissions but not soil nitrous oxide emissions. Soil pH, dissolved organic carbon, ammonia nitrogen, the contents of total phospholipid fatty acid (PLFA), and the ratios of gram‐positive bacteria to gram‐negative bacteria and saturated to monounsaturated PLFAs significantly increased. In addition, nitrate nitrogen and the ratios of fungi to bacteria, total iso‐branched fatty acids to total anteiso‐branched fatty acids, and cyclopropyl to precursor significantly decreased with increasing microplastic addition. The addition of microplastics decreased the abundance of ammonia oxidizing bacteria and nitrite reductase (nirS) but had little effect on the functional genes of ammonia oxidizing archaea, nitrite reductase (nirK), and nitrous oxide reductase. A principal coordinate analysis of the bacterial 16S ribosomal RNA gene and fungal internal transcribed spacer in the microplastic addition treatments revealed that the bacterial and fungal communities formed an obvious cluster. The average abundance of some microbial species with tolerance and degradability to microplastics, such as Nocardioidaceae, Amycolatopsis, Aeromicrobium, Cytophagaceae, Betaproteobacteria, Rhodoplanes, and Mortierella, in the microplastic addition treatments was significantly higher than that of the control treatment. The results suggested that microplastics have obvious influences on microbial communities and may affect global carbon and nitrogen cycles. Environ Toxicol Chem 2021;40:352–365. © 2020 SETAC

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Section: Discussionmentioning

confidence: 99%

Microplastic Addition Alters the Microbial Community Structure and Stimulates Soil Carbon Dioxide Emissions in Vegetable‐Growing Soil

Gao

Yao

et al. 2020

Enviro Toxic and Chemistry

230

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“…D12D and D15D are the key enzymes involved in the synthesis of omega-3 and omega-6 FAs. D12D is responsible for the conversion of oleic acid (OA, 18:1 n-9) to LA, and D15D desaturates LA to ALA [29]. These enzymes are not expressed in all mammals, including humans; as a result, they cannot synthesize LA and ALA de novo and need to obtain these essential FAs from the diet.…”

Section: Activity Of Unsaturated Fatty Acid Desaturasesmentioning

confidence: 99%

Biological Role of Unsaturated Fatty Acid Desaturases in Health and Disease

Czumaj

Śledziński

2020

Nutrients

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Polyunsaturated fatty acids (PUFAs) are considered one of the most important components of cells that influence normal development and function of many organisms, both eukaryotes and prokaryotes. Unsaturated fatty acid desaturases play a crucial role in the synthesis of PUFAs, inserting additional unsaturated bonds into the acyl chain. The level of expression and activity of different types of desaturases determines profiles of PUFAs. It is well recognized that qualitative and quantitative changes in the PUFA profile, resulting from alterations in the expression and activity of fatty acid desaturases, are associated with many pathological conditions. Understanding of underlying mechanisms of fatty acid desaturase activity and their functional modification will facilitate the development of novel therapeutic strategies in diseases associated with qualitative and quantitative disorders of PUFA.

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“…Mammals cannot synthesize linolenic acid (LA). Therefore, LA belongs to the group of essential fatty acids (EFAs) [ 3 ]. One of the most promising EFA sources is microbial lipids, which were proposed to be high-value PUFAs produced during the past two decades [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

“…LA is the specific substrate of Δ6-desaturase and Δ15-desaturase used to generate GLA and α-linolenic acid (ALA). Subsequent elongation and desaturation lead to the generation of long-chain PUFAs, such as EPA and DHA [ 3 ]. Thus, LA is the base for the synthesis of PUFAs.…”

Section: Introductionmentioning

confidence: 99%

Homologous and Heterologous Expression of Delta(12)-Desaturase in Mucor circinelloides Enhanced the Production of Linolenic Acid

et al. 2022

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Linolenic acid (LA) is gaining more interest within the scientific community. This is because it has a potential medical role in reducing the risk of inflammation, carcinogenesis, atherosclerosis and diabetes and is a valuable nutraceutical for human health. The oleaginous fungus Mucor circinelloides produces a high lipid content (36%), including valuable polyunsaturated fatty acids (PUFAs). However, the critical step in which oleic acid (OA) is converted into LA is not efficient at supplying enough substrates for PUFA synthesis. Hence, we propose a method to increase LA production based on genetic engineering. The overexpression of the Δ12-desaturase gene from M. circinelloides and Mortierella alpina increased the LA content and improved the lipid accumulation (from 14.9% to 21.6% in the Δ12-desaturase gene of the M. circinelloides overexpressing strain (Mc-D12MC) and from 14.9% to 18.7% in the Δ12-desaturase gene of M. alpina overexpressing strain (Mc-D12MA)). Additionally, the up-regulated expression levels of these genes targeted the genes involved in NADPH production, implying that the elevated Δ12-desaturase gene may function as a critical regulator of NADPH and lipid synthesis in M. circinelloides. This study provides the first evidence to support the design of metabolic engineering related to LA and PUFA production in M. circinelloides for potential industrial applications.

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Overexpression of delta-12 desaturase in the yeast Schwanniomyces occidentalis enhances the production of linoleic acid

Cited by 17 publications

References 51 publications

Microplastic Addition Alters the Microbial Community Structure and Stimulates Soil Carbon Dioxide Emissions in Vegetable‐Growing Soil

Microplastic Addition Alters the Microbial Community Structure and Stimulates Soil Carbon Dioxide Emissions in Vegetable‐Growing Soil

Biological Role of Unsaturated Fatty Acid Desaturases in Health and Disease

Homologous and Heterologous Expression of Delta(12)-Desaturase in Mucor circinelloides Enhanced the Production of Linolenic Acid

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