Overexpression of CYP6ER1 associated with clothianidin resistance in Nilaparvata lugens (Stål)

Jin, Ruoheng; Mao, Kaikai; Liao, Xun; Xu, Pengfei; Zhao, Li; Ali, Ehsan; Wan, Hu; Li, Jianhong

doi:10.1016/j.pestbp.2018.12.008

Cited by 81 publications

(88 citation statements)

References 34 publications

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“…The enhancement of P450 metabolism activity confers resistance to insecticides in BPH 39 . Among the 54 P450 genes, NlCYP6ER1 seems to metabolize numerous exogenous compounds, thereby participating in BPH resistance to buprofezin, dinotefuran, sulfoxaflor, nitenpyram and clothianidin 16‐19 . In the present study, the susceptibility of transgenic flies overexpressing NlCYP6ER1 to pymetrozine decreased to a certain extent (Table 2).…”

Section: Discussionsupporting

confidence: 48%

“…Once the insect has been exposed to insecticides, the expression of detoxification enzymes may be upregulated or its structure changed, leading to enhanced detoxification activity. Cytochrome P450, as an important class of detoxification enzymes, is widely involved in the resistance of N. lugens to a variety of insecticides, such as imidacloprid, dinotefuran, thiamethoxam, clothianidin, sulfoxaflor, and buprofezin 13,16‐20 . Multiple cytochrome P450 genes ( CYP6ER1 , CYP6AY1 , CYP4CE1 and CYP6CW1 ) of BPH can metabolize imidacloprid and increase BPH resistance to imidacloprid 21‐24 .…”

Section: Introductionmentioning

confidence: 99%

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Mechanism of metabolic resistance to pymetrozine in Nilaparvata lugens: over‐expression of cytochrome P450 CYP6CS1 confers pymetrozine resistance

Wang

Tao

Zhang

et al. 2021

Pest Management Science

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BACKGROUND Pymetrozine is commonly used for the control of Nilaparvata lugens, and resistance to pymetrozine has been frequently reported in the field populations in recent years. However, the mechanism of brown planthopper resistance to pymetrozine is still unknown. RESULTS In this study, a pymetrozine‐resistant strain (PMR) was established, and the potential biochemical resistance mechanism of N. lugens to pymetrozine was investigated. Pymetrozine was synergized by the inhibitor piperonyl butoxide (PBO) in the PMR with 2.83‐fold relative synergistic ratios compared with the susceptible strain (Sus). Compared with the Sus, the cytochrome P450 monooxygenase activity of PMR was increased by 1.7 times, and two P450 genes (NlCYP6CS1 and NlCYP301B1) were found to be significantly overexpressed more than 6.0‐fold in the PMR. Pymetrozine exposure induced upregulation of NlCYP6CS1 expression in the Sus, but the expression of NlCYP301B1 did not change significantly. In addition, RNA interference (RNAi)‐mediated suppression of NlCYP6CS1 gene expression dramatically increased the toxicity of pymetrozine against N. lugens. Moreover, transgenic lines of Drosophila melanogaster expressing NlCYP6CS1 were less susceptible to pymetrozine, and had a stronger ability to metabolize pymetrozine. CONCLUSIONS Taken together, our findings indicate that the overexpression of NlCYP6CS1 is one of the key factors contributing to pymetrozine resistance in N. lugens. And this result is helpful in proposing a management strategy for pymetrozine resistance.

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Section: Discussionsupporting

confidence: 48%

Section: Introductionmentioning

confidence: 99%

Mechanism of metabolic resistance to pymetrozine in Nilaparvata lugens: over‐expression of cytochrome P450 CYP6CS1 confers pymetrozine resistance

Wang

Tao

Zhang

et al. 2021

Pest Management Science

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“…Commonly, increasing P450 enzyme activity in resistant pests is regulated by overexpression of one or more P450 genes (Karunker et al ., 2008; Bao et al ., 2016; Elzaki et al ., 2016). Especially, the P450 genes CYP6AY1 and (or) CYP6ER1 have been identified to be involved in resistance to imidacloprid, nitenpyram, thiamethoxam, clothianidin, dinotefuran, sulfoxaflor and buprofezin in laboratory and field‐collected N. lugens populations (Bass et al ., 2011; Ding et al ., 2013; Bao et al ., 2016; Pang et al ., 2016; Zhang et al ., 2016b; Sun et al ., 2018; Wu et al ., 2018; Jin et al ., 2019; Liao et al ., 2019; Mao et al ., 2019). Similarly, the current study indicated that when compared with SS, significant overexpression of CYP6ER1 was found in all five field populations, which showed resistance to multiple insecticides including clothianidin, dinotefuran, thiamethoxam, imidacloprid, nitenpyram, sulfoxaflor, and buprofezin.…”

Section: Discussionmentioning

confidence: 99%

Current susceptibilities of brown planthopper Nilaparvata lugens to triflumezopyrim and other frequently used insecticides in China

Liao

Gong

et al. 2020

Insect Science

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The brown planthopper is a notorious rice pest in many areas of Asia. The evolution of insecticide resistance in Nilaparvata lugens has become a serious problem in the effective control of this pest in the paddy field. In this article, the current susceptibility of N. lugens field populations to novel mesoionic insecticide triflumezopyrim and major classes of chemical insecticides was determined and compared. The monitoring results indicated that field populations of N. lugens had developed low resistance to triflumezopyrim (resistance ratio, RR: 1.3-7.3-fold) during 2015-2018 in China, and the median lethal concentration values varied from 0.05 to 0.29 mg/L. Additionally, during 2017 to 2018, field populations of N. lugens showed high resistance levels to thiamethoxam (RR: 456.1-1025.6-fold), imidacloprid (RR: 2195.3-6899.0-fold) and buprofezin (RR: 1241.5-4521.7-fold), moderate to high resistance levels to dinotefuran (RR: 97.6-320.1fold), clothianidin (RR: 69.4-230.1-fold) and isoprocarb (RR: 44.1-108.0-fold), and low to moderate levels of resistance to chlorpyrifos (RR: 12.0-29.7-fold) and nitenpyram (RR: 6.9-24.1-fold). In contrast, N. lugens just showed low resistance to sulfoxaflor (RR: 3.3-8.5-fold) and etofenprox (RR: 5.0-9.1-fold) in the field. Additionally, the P450 gene CYP6ER1 was found to be significantly overexpressed in all five field populations of N. lugens collected in 2018 when compared with a laboratory susceptible strain. Our findings will provide useful information to delay the evolution of insecticide resistance in N. lugens.

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“…Insecticide selection pressure from agriculture is generally regarded as an important early driver of insecticide resistance in malaria vectors [30, 31]. According to the Insecticide Resistance Action Committee (IRAC), neonicotinoid resistance has been reported to at least 12 species of crop pest due to agricultural use in Asia, Europe and North America, with increased expression of various P450 genes implicated in several species [32–34]. Neonicotinoids are undoubtedly becoming more widely utilized for agricultural pest control in Africa.…”

Section: Discussionmentioning

confidence: 99%

Susceptibility testing of Anopheles malaria vectors with the neonicotinoid insecticide clothianidin; results from 16 African countries, in preparation for indoor residual spraying with new insecticide formulations

Oxborough

Seyoum

Yihdego

et al. 2019

Malar J

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Background In 2017, more than 5 million house structures were sprayed through the U.S. President’s Malaria Initiative, protecting more than 21 million people in sub-Saharan Africa. New IRS formulations, SumiShield™ 50WG and Fludora Fusion™ WP-SB, became World Health Organization (WHO) prequalified vector control products in 2017 and 2018, respectively. Both formulations contain the neonicotinoid active ingredient, clothianidin. The target site of neonicotinoids represents a novel mode of action for vector control, meaning that cross-resistance through existing mechanisms is less likely. In preparation for rollout of clothianidin formulations as part of national IRS rotation strategies, baseline susceptibility testing was conducted in 16 countries in sub-Saharan Africa. Methods While work coordinated by the WHO is ongoing to develop a suitable bottle bioassay procedure, there was no published guidance regarding clothianidin susceptibility procedures or diagnostic concentrations. Therefore, a protocol was developed for impregnating filter papers with 2% w/v SumiShield™ 50WG dissolved in distilled water. Susceptibility tests were conducted using insectary-reared reference Anopheles and wild collected malaria vector species. All tests were conducted within 24 h of treating papers, with mortality recorded daily for 7 days, due to the slow-acting nature of clothianidin against mosquitoes. Anopheles gambiae sensu lato (s.l.) adults from wild collected larvae were tested in 14 countries, with wild collected F 0 Anopheles funestus s.l. tested in Mozambique and Zambia. Results One-hundred percent mortality was reached with all susceptible insectary strains and with wild An. gambiae s.l. from all sites in 11 countries. However, tests in at least one location from 5 countries produced mortality below 98%. While this could potentially be a sign of clothianidin resistance, it is more likely that the diagnostic dose or protocol requires further optimization. Repeat testing in 3 sites in Ghana and Zambia, where possible resistance was detected, subsequently produced 100% mortality. Results showed susceptibility to clothianidin in 38 of the 43 sites in sub-Saharan Africa, including malaria vectors with multiple resistance mechanisms to pyrethroids, carbamates and organophosphates. Conclusions This study provides an interim diagnostic dose of 2% w/v clothianidin on filter papers which can be utilized by National Malaria Control Programmes and research organizations until the WHO concludes multi-centre studies and provides further guidance. Electronic supplementary material The online version of this article (10.1186/s12936-019-2888-6) contains supplementary material, which is available to authorized users.

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Overexpression of CYP6ER1 associated with clothianidin resistance in Nilaparvata lugens (Stål)

Cited by 81 publications

References 34 publications

Mechanism of metabolic resistance to pymetrozine in Nilaparvata lugens: over‐expression of cytochrome P450 CYP6CS1 confers pymetrozine resistance

Mechanism of metabolic resistance to pymetrozine in Nilaparvata lugens: over‐expression of cytochrome P450 CYP6CS1 confers pymetrozine resistance

Current susceptibilities of brown planthopper Nilaparvata lugens to triflumezopyrim and other frequently used insecticides in China

Susceptibility testing of Anopheles malaria vectors with the neonicotinoid insecticide clothianidin; results from 16 African countries, in preparation for indoor residual spraying with new insecticide formulations

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