Overexpression of Cyclooxygenase-2 Correlates With Chromosomal Gain at the Cyclooxygenase-2 Locus and Decreased Patient Survival in Advanced Colorectal Carcinomas

Knösel, Thomas; Ye, Yu; Stein, Ulrike; Schwabe, Holger; Schlüns, Karsten; Schlag, Peter M.; Dietel, Manfred; Petersen, Iver

doi:10.1007/s10350-003-0008-7

Cited by 27 publications

(24 citation statements)

References 22 publications

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“…One paper reported a significantly worse prognosis in patients with a membranous HER2 reaction of 2+/3+, found in 51% of a group of 74 patients with CRC. The authors used an immunohistochemical assay (Herceptest; Dako) with a cut-off point of 10% of cells [10]. Other authors using similar criteria for estimation observed membranous HER2 overexpression in only 5% of 96 colorectal tumors.…”

Section: Discussionmentioning

confidence: 99%

Expression of HER2 in Colorectal Cancer Does Not Correlate with Prognosis

et al. 2010

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Abstract. Estimation of HER2 membranous expression is routinely used in breast and gastric cancers, as both a prognostic and a predictive factor. To date there is no evidence for similar application of HER2 expression in colorectal cancer (CRC) cells. In CRC, HER2 is sometimes overexpressed in the cell membrane and very often in the cytoplasm. This study was conducted to determine possible correlations between both membranous and cytoplasmatic expression of HER2 in CRC cells and the outcome of the disease. The prognostic significance of combined staining intensity in the cell membrane and cytoplasm in the entire CRC cell was also investigated. HER2 expression in resectable colorectal adenocarcinoma cells was evaluated by immunohistochemistry in specimens taken from 202 patients. The percentage of cancer cells with membranous or cytoplasmatic reactions and the staining intensity of the reaction in the whole cell were recorded. A membranous reaction was present in 27% of cases, and cytoplasmatic reaction in 66% of cases. The total staining intensity in the entire cell was evaluated as moderate (2+) in 32% of cases and strong (3+) staining in 15%. There was no correlation found between either membranous or cytoplasmatic HER2 expression and survival. Furthermore combined staining intensity did not provide any prognostic information. We conclude that HER2 expression in CRC does not correlate with prognosis.

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Section: Discussionmentioning

confidence: 99%

Expression of HER2 in Colorectal Cancer Does Not Correlate with Prognosis

et al. 2010

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“…all slides were read by two pathologists (t. Knösel and i. Petersen) who were blinded to the clinical information. immunohistochemistry was scored semiquantitatively as negative (score 0), weak (score 1), moderate (score 2) or strong (score 3) as previously described (15). for statistical evaluation, scores 0 was considered as negative, while scores 1, 2 and 3 together as positive.…”

Section: Methodsmentioning

confidence: 99%

Human complement factor H is a novel diagnostic marker for lung adenocarcinoma

Petersen¹

2011

Int J Oncol

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Abstract.Human complement factor H (cfH), a central complement control protein, is a member of the regulators of complement activation family. recent studies suggested that cfH may play a key role in the resistance of complementmediated lysis in various cancer cells. in this study, we investigated the role of cfH in human lung cancer. expression of cfH was analyzed in lung cancer cell lines by rt-Pcr, Western blotting and immunofluorescence. In primary lung tumors, the protein expression of cfH was evaluated by immunohistochemistry (iHc) on tissue microarray (tMa). Binding of cfH to lung cancer cells was detected by flow cytometry. mrna expression of cfH was detected in 6 out of 10 non-small cell lung cancer (nSclc) cell lines, but in none of the small cell lung cancer (Sclc) cell lines. in line with Western blotting, immunofluorescence analysis demonstrated cfH protein expression in 3 nSclc cell lines, and the immunoreaction was mainly associated with cell cytoplasm and membrane. in primary lung tumors, 54 out of 101 samples exhibited high expression of cfH and high expression was significantly correlated with lung adenocarcinoma (p=0.009). also, in adenocarcinoma of the lung, Kaplan-Meier survival analysis showed a tendency that cfH-positive tumors had worse prognosis in comparison to cfH-negative tumors (p=0.082). Additionally, shorter survival time of patients with adenocarcinoma (<20 months) was associated with higher staining of CFH (p=0.033). Our data showed that non-small cell lung cancer cells expressed and secreted cfH. cfH might be a novel diagnostic marker for human lung adenocarcinoma.

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“…COX-2 expression may also be induced in endothelial and smooth muscle cells and in fibroblasts after stimulation by growth factors, cytokines, lipopolysaccharides and tumor promoters [7,8]. Many studies have demonstrated the overexpression of COX-2 in cells of different human tumors, mostly of epithelial origin like cancers of the colon and rectum [9][10][11][12][13], breast [14,15], stomach [16,17], lung [18], bladder [19][20][21], head and neck [22][23][24], ovary [25,26], cervix [27,28], prostate [29] and pancreas [30].…”

Section: Introduction Introduction Introduction Introduction Introducmentioning

confidence: 99%

Different expression of cyclooxygenase-2 (COX-2) in selected nonmelanocytic human cutaneous lesions

Kuźbicki¹,

Lange²,

Stanek-Widera³

et al. 2011

Folia Histochem Cytobiol.

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Overexpression of Cyclooxygenase-2 Correlates With Chromosomal Gain at the Cyclooxygenase-2 Locus and Decreased Patient Survival in Advanced Colorectal Carcinomas

Abstract: We conclude that cyclooxygenase-2 is related with tumor progression and metastasis in colorectal cancer, which can be observed on protein level, and reflects chromosomal gain at the locus at 1q25.2-q25.3.

Cited by 27 publications

References 22 publications

Expression of HER2 in Colorectal Cancer Does Not Correlate with Prognosis

Expression of HER2 in Colorectal Cancer Does Not Correlate with Prognosis

Human complement factor H is a novel diagnostic marker for lung adenocarcinoma

Different expression of cyclooxygenase-2 (COX-2) in selected nonmelanocytic human cutaneous lesions

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