Overexpression of cyclin Y in non-small cell lung cancer is associated with cancer cell proliferation

Yue, Wentao; Zhao, Xiaoting; Zhang, Lína; Liu, Zhidong; Ma, Li; Jia, Wenyun; Zhang, Qian; Wang, Yue; Yang, Xuehui; Xu, Shaofa

doi:10.1007/s11427-010-0090-8

Cited by 8 publications

(10 citation statements)

References 11 publications

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“…Recently, a study conducted by Sun et al reported that the interaction between CCNY and serine/threonine-protein kinase PFTAIRE-1 (PFTK1) might affect HCC through a non-canonical Wnt signaling pathway [ 28 ]. A further study by Yue et al showed that the expression of CCNY was significantly increased in NSCLC [ 16 ]. A previous study has reported that CCNY can enhance cell proliferation, colony formation, cell progression and tumorigenesis in glioma cells [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

“…However, there are still limited studies on the molecular mechanism associated with CCNY expression and HCC, although the relationship between CCNY and other human cancers have been studied [ 14 – 17 ]. Recent studies have shown that CCNY expression is associated with increased cell proliferation in human non small-cell lung cancer (NSCLC), and serum levels of anti-CCNY antibodies are increased in patients with NSCLC when compared with normal controls [ 14 , 16 , 17 ]. Expression of CCNY has also been shown to be increased in human colorectal carcinoma cell lines derived from metastatic carcinoma when compared with cell lines derived from non-metastatic colorectal carcinoma, suggesting a possible role of CCNY in tumor invasion and metastasis [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

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Cyclin Y Modulates the Proliferation, Invasion, and Metastasis of Hepatocellular Carcinoma Cells

Shi

Zhang

et al. 2018

Med Sci Monit

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BackgroundCyclin Y (CCNY) is a member of the cyclin family of proteins that regulate the cell cycle. The aims of this study were to compare the expression of CCNY in normal liver and human hepatocellular carcinoma (HCC), in normal and HCC cell lines, and in mouse HCC tumor xenografts.Material/MethodsTumor tissues from 55 patients diagnosed with HCC were studied for CCNY expression. Human HCC cell lines, SK-Hep1, HepG2, HEP3B, HuH7 and L02 were studied using the MTT cell proliferation assay, cell apoptosis, transwell and wound healing assays. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blot were used to measure CCNY expression. Indirect immunofluorescence was used to assess cell apoptosis. In vivo xenograft mouse model was constructed and examined histologically.ResultsExpression of CCNY in human HCC tumor tissues was significantly increased when compared with adjacent normal liver (all P<0.05). HCC cells grown in vitro showed significantly increased expression of CCNY, cell proliferation, and migration, and a reduced rate of apoptosis, compared with cells with CCNY knockdown (siRNA) (all P<0.05). In the xenograft mouse model, tumor volume and weight in the CCNY overexpression group were significantly increased, compared with CCNY knockdown (siRNA) group (all P<0.05).ConclusionsIn tissue samples of human HCC, and human HCC cell lines, increased expression of CCNY was significantly associated with cell proliferation and migration. Further studies are recommended to evaluate the role of CCNY as a potential diagnostic biomarker or target for treatment in human HCC.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cyclin Y Modulates the Proliferation, Invasion, and Metastasis of Hepatocellular Carcinoma Cells

Shi

Zhang

et al. 2018

Med Sci Monit

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“…Many studies have described how cyclins A, B, D, and E and their respective autoantibodies are overexpressed in various cancers, including lung cancer [14, 16–21]. Although CCNY was identified as a novel member of the cyclin family, almost nothing is known about the clinical value of the CCNY protein and anti-CCNY autoantibodies, and only a few studies have reported that the levels of mRNA for CCNY are upregulated in glioma cells and NSCLC tissues [11, 12, 22]. In the present study, we initially used immunohistochemistry to measure the expression levels of CCNY protein in 39 pairs of NSCLC tissues and paratumorous tissues.…”

Section: Discussionmentioning

confidence: 99%

Serum Anti-CCNY Autoantibody Is an Independent Prognosis Indicator for Postoperative Patients with Early-Stage Nonsmall-Cell Lung Carcinoma

Yue

Teng

et al. 2013

Disease Markers

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Cyclin Y (CCNY) is a novel cyclin and almost nothing is known about its role in human cancers. To investigate the clinical significance of serum anti-CCNY autoantibodies in nonsmall-cell lung carcinoma (NSCLC), the serum levels of CCNY protein in 264 patients with NSCLC, 103 patients with tuberculosis, and 89 healthy controls were analyzed by immunohistochemistry. The result shows that, compared with normal lung tissues, the NSCLC tissues contained higher levels of CCNY protein. The levels of anti-CCNY autoantibodies were higher in the sera of the patients with NSCLC than in the sera of the healthy controls (P < 0.001) or the patients with tuberculosis (P = 0.027). Moreover, in a Cox regression analysis, anti-CCNY autoantibody was an independent factor that predicted poor prognosis for postoperative patients with early-stage NSCLC (P = 0.026) as well as for those with distant metastasis (P = 0.012). Our data indicated that Anti-CCNY autoantibody may be useful as a latent tumor marker to facilitate diagnosis and may represent a novel prognostic indicator for patients with early stage NSCLC.

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“…Recent studies have shown that CCNY protein may play an important role in cancer cell proliferation and metastasis 21,22. Our previous study have shown that CCNY protein is overexpressed in NSCLC tissues 25.…”

Section: Discussionmentioning

confidence: 90%

“…CCNY acts as a growth factor sensor to integrate extracellular signals within the cell cycle machinery 15–18. Recent studies have shown that CCNY may play an important role in cancer cell proliferation and metastasis, including breast cancer,19 ovarian cancer,20 lung cancer,21,22 colon cancer,23 glioma,24 etc. We previously found that CCNY mRNA was overexpressed in numerous lung cancer cell lines,21 and that the level of CCNY protein was significantly increased in lung cancer tissues 25.…”

Section: Introductionmentioning

confidence: 99%

<p>Establishing a detection method for CCNY: a potentially significant clinical investigative marker in NSCLC patients</p>

Teng

et al. 2019

OTT

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BackgroundCCNY, a novel cyclin family member, plays an increasingly important role in the progression of tumor invasion and metastasis, including lung cancer. However, the clinical significance of CCNY in non-small-cell lung cancer (NSCLC) patients is unknown.Patients and methodsWe prepared CCNY monoclonal antibodies, validated specific peptides by a peptide array, and established a double-antibody sandwich ELISA detection method. Then, we measured CCNY levels in 100 NSCLC patients and 50 healthy controls. A blinded validation was subsequently performed in 399 NSCLC patients and 150 healthy controls.ResultsWe successfully prepared two specific mouse anti-human CCNY monoclonal antibodies and established a reliable and stable detection method. In the training set, serum CCNY was markedly increased in the NSCLC patients (P<0.05) with an integrated area under the curve of 0.751. With further analysis of the CCNY levels, there were no differences in age, sex, smoking status, tumor location, histologic subtype, or tumor size, but differences were observed in lymphatic (P<0.001) and distant (P<0.001) metastases in NSCLC patients. The CCNY[+] patients had a shorter survival time and progression-free survival than CCNY[−] patients at 3-year follow-up (P<0.001). The results were confirmed by the validation set.ConclusionOur study suggests that CCNY may be useful as a latent tumor marker to facilitate diagnosis and may be an effective indicator of tumor aggressiveness, playing an important role in the prognosis of NSCLC patients.

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Overexpression of cyclin Y in non-small cell lung cancer is associated with cancer cell proliferation

Cited by 8 publications

References 11 publications

Cyclin Y Modulates the Proliferation, Invasion, and Metastasis of Hepatocellular Carcinoma Cells

Cyclin Y Modulates the Proliferation, Invasion, and Metastasis of Hepatocellular Carcinoma Cells

Serum Anti-CCNY Autoantibody Is an Independent Prognosis Indicator for Postoperative Patients with Early-Stage Nonsmall-Cell Lung Carcinoma

<p>Establishing a detection method for CCNY: a potentially significant clinical investigative marker in NSCLC patients</p>

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