Overexpression of cotton Trihelix transcription factor GhGT-3b_A04 enhances resistance to Verticillium dahliae and affects plant growth in Arabidopsis thaliana

Mao, Haoming; Zhang, Wenqing; Lv, Junyuan; Yang, Jiaxiang; Yang, Shaobo; Jia, Bing; Song, Jikun; Wu, Man; Pei, Wenfeng; Ma, Jianjiang; Zhang, Bingbing; Zhang, Jinfa; Wang, Li; Yu, Jie-Ae

doi:10.1016/j.jplph.2023.153947

Cited by 6 publications

(2 citation statements)

References 68 publications

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“…GT1 belongs to the trihelix transcription factor family, which is named after its conserved three-helix structural domain (helix-loop-helix-loop-helix). Trihelix transcription factors are widely found in a number of plant genomes and are involved in various biological processes, such as embryogenesis, formation of perianth, stomata, and seeds [46][47][48]. They are also found to participate in regulation of plant responses to stresses especially abiotic tresses [49].…”

Section: Discussionmentioning

confidence: 99%

E3 Ubiquitin Ligase PUB23 in Kiwifruit Interacts with Trihelix Transcription Factor GT1 and Negatively Regulates Immune Responses against Pseudomonas syringae pv. actinidiae

Wang,

Zhang

et al. 2024

IJMS

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Kiwifruit bacterial canker caused by Pseudomonas syringae pv. actinidiae (Psa) is the most serious disease threatening kiwifruit production. Our previous study found genes encoding the U-box containing proteins were significantly regulated by Psa infection. Here, we report a U-box type E3 ubiquitin ligase PUB23 in kiwifruit which acts as a negative regulator of immune responses against Psa. PUB23 was found to physically interact with GT1, a trihelix transcription factor, in vitro and in vivo. The expression of GT1 was up-regulated in PUB23-silenced plants, indicating that interacting with PUB23 may directly or indirectly suppress GT1 expression. The silencing of PUB23 led to enhanced immune responses of PAMP-triggered immunity (PTI), including a higher expression level of defense marker genes PR1 and RIN4, and increased accumulation of hydrogen peroxide and superoxide anion. Our results reveal a negative role PUB23 plays in kiwifruit immune responses against Psa and may regulate gene expression by interacting with GT1.

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Section: Discussionmentioning

confidence: 99%

E3 Ubiquitin Ligase PUB23 in Kiwifruit Interacts with Trihelix Transcription Factor GT1 and Negatively Regulates Immune Responses against Pseudomonas syringae pv. actinidiae

Wang,

Zhang

et al. 2024

IJMS

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“…Previous reports proved that altering plant development with trihelix factors contributes to pathogen susceptibility or resistance. GhGT-3b was strongly induced by Verticillium dahlia and the heterologous expression of GhGT-3b in Arabidopsis enhanced resistance to Verticillium dahlia but inhibited the growth of rosette leaves [5]. ARABIDOPSIS SH4-RELATED 3 (ASR3) overexpressed plants were smaller than the control but enhanced susceptibility to infections of Pseudomonas syringae pv tomato DC3000 and Pseudomonas syringae pv maculicola ES4326 [6].…”

Section: Introductionmentioning

confidence: 99%

SlbHLH22-Induced Hypertrophy Development Is Related to the Salt Stress Response of the GTgamma Gene in Tomatoes

Cui,

Yu,

Bai

et al. 2023

Metabolites

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Hypertrophy development induced by the overexpression of SlbHLH22 (also called SlUPA-like) was susceptible to Xanthomonas in tomatoes. Transcriptome and metabolome analyses were performed on the hypertrophy leaves of a SlbHLH22-overexpressed line (OE) and wild type (WT) to investigate the molecular mechanism. Metabolome analysis revealed that six key metabolites were over-accumulated in the OE, including Acetylserine/O-Acetyl-L-serine, Glucono-1,5-lactone, Gluconate, 2-Oxoglutarate, and Loganate, implying that the OE plants increased salt or oxidant resistance under normal growth conditions. The RNA-seq analysis showed the changed expressions of downstream genes involved in high-energy consumption, photosynthesis, and transcription regulation in OE lines, and we hypothesized that these biological processes were related to the GTgamma subfamily of trihelix factors. The RT-PCR results showed that the expressions of the GTgamma genes in tomatoes, i.e., SlGT-7 and SlGT-36, were suppressed in the hypertrophy development. The expression of the GTgamma gene was downregulated by salinity, indicating a coordinated role of GTgamma in hypertrophy development and salt stress. Further research showed that both SlGT-7 and SlGT-36 were highly expressed in leaves and could be significantly induced by abscisic acid (ABA). The GTgamma protein had a putative phosphorylation site at S96. These results suggested GTgamma’s role in hypertrophy development by increasing the salt resistance.

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Genome-wide identification of trihelix transcription factor family genes in pear (Pyrus bretschneideri) and functional characterization of PbrGT15 in black spot resistance

Zhang,

Xie,

et al. 2023

HORTIC. ADV.

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Pear (Pyrus bretschneideri), a valuable widely cultivated fruit, faces significant economic losses due to black spot disease caused by Alternaria alternate (Fr.) Keissl. Trihelix transcription factors (TFs) are crucial in regulating plant defense and autoimmunity. This study aimed to analyze the trihelix transcription factor (GT) genes within pear through genome-wide identification, phylogenetic, gene structure, synteny, and cis-acting elements analyses. Among the 31 trihelix genes, 28 were on 12 known chromosomes, while the remaining 3 were located on unknown chromosomes. These genes were categorized into five clades: SIP1, GTγ, GT1, GT2 and SH4, containing 7, 2, 9, 11 and 2 genes, respectively. Synteny analysis indicated eight duplicated gene pairs. Based on the expression pattern of PbGT genes in seven tissues from the database, the PbGT genes of the GT2 clade were selected for further investigation. The quantitative reverse transcriptase–polymerase chain reaction confirmed that PbrGT5, PbrGT6, PbrGT15 and PbrGT16 correlated with black spot disease resistance. Notably, the salicylic acid (SA) treatment significantly upregulated the expression levels of PbrGT10, PbrGT13, PbrGT15 and PbrGT23. Among these, PbrGT15 showed the highest induction to both SA and black spot infection. Subcellular localization demonstrated that PbrGT15 functions as a nuclear protein. Virus-induced gene silencing of PbrGT15 increased pear plants' susceptibility to black spot disease, indicating its pivotal role in enhancing resistance. These results indicated that PbrGT15 positively regulated black spot disease resistance in pears.

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Overexpression of cotton Trihelix transcription factor GhGT-3b_A04 enhances resistance to Verticillium dahliae and affects plant growth in Arabidopsis thaliana

Cited by 6 publications

References 68 publications

E3 Ubiquitin Ligase PUB23 in Kiwifruit Interacts with Trihelix Transcription Factor GT1 and Negatively Regulates Immune Responses against Pseudomonas syringae pv. actinidiae

E3 Ubiquitin Ligase PUB23 in Kiwifruit Interacts with Trihelix Transcription Factor GT1 and Negatively Regulates Immune Responses against Pseudomonas syringae pv. actinidiae

SlbHLH22-Induced Hypertrophy Development Is Related to the Salt Stress Response of the GTgamma Gene in Tomatoes

Genome-wide identification of trihelix transcription factor family genes in pear (Pyrus bretschneideri) and functional characterization of PbrGT15 in black spot resistance

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