Overexpression of Citrus reticulata SAMT in Nicotiana tabacum increases MeSA volatilization and decreases Xylella fastidiosa symptoms

Gómez, Laura; Teixeira-Silva, Natália Sousa; Caserta, Raquel; Takita, Marco Aurélio; Marques, Márcia Ortiz Mayo; Souza, Alessandra Alves de

doi:10.1007/s00425-020-03511-1

Cited by 7 publications

(16 citation statements)

References 65 publications

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“…was used (Lacombe et al, 2010;Zipfel et al, 2006). Transgenic tobacco was produced by Agrobacterium-mediated transformation with the strain GV3101, as previously described (Gómez et al, 2020). Vigorous shoots grew in selective media (MS/2 supplemented with 2 μg mL -1 phosphinothricin) were transferred to pots containing 2:1 substrate/vermiculite and kept in an acclimatization room at 28 ºC for 21 days.…”

Section: The Binary Vector Pearlygate103 Containing the Open Reading mentioning

confidence: 99%

TheArabidopsisimmune receptor EFR increases resistance to the bacterial pathogensXanthomonasandXylellain transgenic sweet orange

Mitre

Teixeira-Silva

Rybak

et al. 2021

Preprint

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SummaryPlants employ cell surface receptors to recognize pathogen (or microbe)-associated molecular patterns (PAMPs/MAMPs), which are crucial for immune system activation. The well-studied Arabidopsis thaliana ELONGATION FACTOR-TU RECEPTOR (EFR) recognizes the conserved bacterial PAMP EF-Tu, and the derived peptides elf18 and elf26. The interfamily transfer of EFR has been shown to increase disease resistance in several crops, such as tomato, rice, wheat, and potato. Here, we generated sweet orange (Citrus sinensis) transgenic lines expressing EFR to test if it would confer broad-spectrum resistance against two important citrus bacterial diseases: citrus canker and citrus variegated chlorosis (CVC). Independent EFR transgenic lines gained responsiveness to elf18 and elf26 peptides from Xanthomonas citri and Xylella fastidiosa, as measured by reactive oxygen species (ROS) production, mitogen-activated protein kinase (MAPK) activation and defense gene expression. Consistently, infection assays showed that Citrus-EFR transgenic plants were more resistant to citrus canker and CVC. Our results show that the EFR immune receptor can improve plant immunity in a perennial crop against bacterial pathogens, opening perspectives to engineer durable broad-spectrum disease resistance under field conditions.

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Section: The Binary Vector Pearlygate103 Containing the Open Reading mentioning

confidence: 99%

TheArabidopsisimmune receptor EFR increases resistance to the bacterial pathogensXanthomonasandXylellain transgenic sweet orange

Mitre

Teixeira-Silva

Rybak

et al. 2021

Preprint

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“…We previously demonstrated that the overexpression of the SAMT gene from Citrus reticulata (CrSAMT) in tobacco (Nicotiana tabacum) showed a significant symptom reduction in response to Xylella fastidiosa subsp. pauca ( Gómez et al, 2020 ). In addition, CrSAMT -transgenic plants increased MeSA volatilization, promoting the induction of PR1 in distal tissues and in neighboring wild-type plants ( Gómez et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…pauca ( Gómez et al, 2020 ). In addition, CrSAMT -transgenic plants increased MeSA volatilization, promoting the induction of PR1 in distal tissues and in neighboring wild-type plants ( Gómez et al, 2020 ). Recently, overexpression of CsSAMT1 (homologous to CrSAMT ) in Wanjincheng oranges ( C. sinensis ) was demonstrated to enhance the tolerance to Huanglongbing caused by Candidatus Liberibacter asiaticus (LAS) in citrus species ( Zou et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…We previously showed that C. reticulata CrSAMT belongs to the SABATH family of methyltransferases containing SAM- and SA-binding residues ( Gómez et al, 2020 ). An identical copy of CrSAMT is present in C. sinensis ( CsSAMT ) [Cs1g24440.1 v2.0 (HZAU), orange1.1g017514m v1.1 (JGI) and XM_006466773 (NCBI)] ( Gómez et al, 2020 ). Thus, considering previous reports regarding the role of SAMT in the defense response against citrus bacterial pathogens ( Gómez et al, 2020 ; Zou et al, 2021 ), we generated stable Valencia and Hamlin sweet orange transgenic lines overexpressing CsSAMT and evaluated local and systemic resistance against X. citri .…”

Section: Introductionmentioning

confidence: 99%

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Overexpression of CsSAMT in Citrus sinensis Induces Defense Response and Increases Resistance to Xanthomonas citri subsp. citri

et al. 2022

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Citrus canker is a destructive disease caused by Xanthomonas citri subsp. citri, which affects all commercial sweet orange (Citrus sinensis [L.] Osbeck) cultivars. Salicylic acid (SA) and systemic-acquired resistance (SAR) have been demonstrated to have a crucial role in mediating plant defense responses against this phytopathogen. To induce SAR, SA is converted to methyl salicylate (MeSA) by an SA-dependent methyltransferase (SAMT) and translocated systemically to prime noninfected distal tissues. Here, we generated sweet orange transgenic plants (based on cvs. Hamlin and Valencia) overexpressing the SAMT gene from Citrus (CsSAMT) and evaluated their resistance to citrus canker. We obtained four independent transgenic lines and confirmed their significantly higher MeSA volatilization compared to wild-type controls. Plants overexpressing CsSAMT showed reduced symptoms of citrus canker and bacterial populations in all transgenic lines without compromising plant development. One representative transgenic line (V44SAMT) was used to evaluate resistance response in primary and secondary sites. Without inoculation, V44SAMT modulated CsSAMT, CsNPR1, CsNPR3, and CsWRKY22 expression, indicating that this plant is in a primed defense status. The results demonstrate that MeSA signaling prompts the plant to respond more efficiently to pathogen attacks and induces immune responses in transgenic plants at both primary and secondary infection sites.

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Discovery of specific catalytic activity toward IAA/FA by LaSABATHs based on genome-wide phylogenetic and enzymatic analysis of SABATH gene family from Larix kaempferi

Zhuge

Xiu

et al. 2023

International Journal of Biological Macromolecules

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Overexpression of Citrus reticulata SAMT in Nicotiana tabacum increases MeSA volatilization and decreases Xylella fastidiosa symptoms

Cited by 7 publications

References 65 publications

TheArabidopsisimmune receptor EFR increases resistance to the bacterial pathogensXanthomonasandXylellain transgenic sweet orange

TheArabidopsisimmune receptor EFR increases resistance to the bacterial pathogensXanthomonasandXylellain transgenic sweet orange

Overexpression of CsSAMT in Citrus sinensis Induces Defense Response and Increases Resistance to Xanthomonas citri subsp. citri

Discovery of specific catalytic activity toward IAA/FA by LaSABATHs based on genome-wide phylogenetic and enzymatic analysis of SABATH gene family from Larix kaempferi

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