Overexpression of Brassica campestris BcICE1 gene increases abiotic stress tolerance in tobacco

Zhang, Tengguo; Mo, Jiangnan; Zhou, Kuanjiu; Chang, Yan; Zigang, Liu

doi:10.1016/j.plaphy.2018.09.039

Cited by 31 publications

(21 citation statements)

References 22 publications

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“…ZjbHLH2 and ZjbHLH4 were expressed at higher levels at earlier flower development stages. These two genes belong to the ICE1 branch, which can regulate lateral bud growth [35]. A similar function was also confirmed by homologous protein interactions (Fig.…”

Section: Discussionmentioning

confidence: 57%

“…ICE1 (homolog of ZjbHLH2, 3, and 4) could interact with FMA (homolog of ZjbHLH34), SPCH (homolog of ZjbHLH35) and MUTE (homolog of ZjbHLH36) could regulate stomatal differentiation [33]. Moreover, ICE1 also regulated lateral bud growth and plant stress response [34, 35], and LRL1 (homolog of ZjbHLH80), RHD6 (homolog of ZjbHLH89) and RSL2 (homolog of ZjbHLH90) were involved in the regulation of root hair development [36, 37]. These results further proved the functional diversity of ZjbHLH genes.…”

Section: Resultsmentioning

confidence: 99%

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Genome-wide analysis of the bHLH gene family in Chinese jujube (Ziziphus jujuba Mill.) and wild jujube

Gao

Xue

et al. 2019

BMC Genomics

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Background: The bHLH (basic helix-loop-helix) transcription factor is one of the largest families of transcription factors in plants, containing a large number of members with diverse functions. Chinese jujube (Ziziphus jujuba Mill.) is the species with the highest economic value in the family Rhamnaceae. However, the characteristics of the bHLH family in the jujube genome are still unclear. Hence, ZjbHLHs were first searched at a genome-wide level, their expression levels under various conditions were investigated systematically, and their protein-protein interaction networks were predicted. Results: We identified 92 ZjbHLHs in the jujube genome, and these genes were classified into 16 classes according to bHLH domains. Ten ZjbHLHs with atypical bHLH domains were found. Seventy ZjbHLHs were mapped to but not evenly distributed on 12 pseudo-chromosomes. The domain sequences among ZjbHLHs were highly conserved, and their conserved residues were also identified. The tissue-specific expression of 37 ZjbHLH genes in jujube and wild jujube showed diverse patterns, revealing that these genes likely perform multiple functions. Many ZjbHLH genes were screened and found to be involved in flower and fruit development, especially in earlier developmental stages. A few genes responsive to phytoplasma invasion were also verified. Based on protein-protein interaction prediction and homology comparison, protein-protein interaction networks composed of 92 ZjbHLHs were also established. Conclusions: This study provides a comprehensive bioinformatics analysis of 92 identified ZjbHLH genes. We explored their expression patterns in various tissues, the flowering process, and fruit ripening and under phytoplasma stress. The protein-protein interaction networks of ZjbHLHs provide valuable clues toward further studies of their biological functions.

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Section: Discussionmentioning

confidence: 57%

Section: Resultsmentioning

confidence: 99%

Genome-wide analysis of the bHLH gene family in Chinese jujube (Ziziphus jujuba Mill.) and wild jujube

Gao

Xue

et al. 2019

BMC Genomics

View full text Add to dashboard Cite

show abstract

“…Three groups of seedlings at a similar developmental stage (30 transgenic seedlings and 30 untransformed control seedlings per group) were subjected to low temperature (4 °C), salinity (300 mM NaCl) and drought (without water for 2, 4, 6, 8, 10, 12 or 14 days).Chlorophyll content [39], malondialdehyde (MDA) content (TBA assay) [40,41], proline (Pro) content (ninhydrin assay) [42], leaf membrane permeability (relative conductivity assay) [43] and morphology were investigated for each treatment. Physiological indices and gene function were statistically analyzed using SAS6.12.…”

Section: Analysis Of Stress Tolerance and Gsltp Functionmentioning

confidence: 99%

GsLTP, a Non-Specific Lipid Transfer Protein from Glycine soja, Enhances Drought and Salt Stress Tolerance in Transgenic Tobacco

Fan

Liu

Cao

et al. 2020

Preprint

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Background Lipid transfer proteins (LTPs) mainly exist in plant cell walls where they make an important impact in the transport of diverse lipophilic compounds. Although the functions of several LTPS in different plant species have been identified, little is known about the biochemical and enzymatic activities of LTP family members in soybeans. Results Herein, GsLTP was identified from Glycine soja using soybean gene microarray expression and screened in a salt stress expressed sequence tag (EST) library. The 369 bp open reading frame (ORF) encodes a protein of 122 amino acids, and the cDNA sequence shares similarity with LTP genes in other plants such as soybean (93%) and Vigna (72%). The function of the gene was characterized in transgenic tobacco. Various physiological characteristics under different environmental stresses were investigated. We discovered that over-expression of GsLTP enhanced tolerance to drought and salt stresses. Conclusions Our results indicate that GsLTP plays an important role in multiple abiotic signalling pathways, and provide a theoretical basis and practical gene resource for crop breeding.

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“…Three groups of seedlings at a similar developmental stage (30 transgenic seedlings and 30 untransformed control seedlings per group) were subjected to low temperature (4°C), salinity (300 mM NaCl) and drought (without water for 2, 4, 6, 8, 10, 12 or 14 days). Chlorophyll content [39], malondialdehyde (MDA) content (TBA assay) [40,41], proline (Pro) content (ninhydrin assay) [42], leaf membrane permeability (relative conductivity assay) [43] and morphology were investigated for each treatment. Physiological indices and gene functions were statistically analyzed using SAS6.12.…”

Section: Analysis Of Stress Tolerance and Gsltp Functionmentioning

confidence: 99%

GsLTP, a Non-Specific Lipid Transfer Protein from Glycine soja, Enhances Drought and Salt Stress Tolerance in Transgenic Tobacco

JinPing

Liu

Cao

et al. 2020

Preprint

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Background: Lipid transfer proteins (LTPs) mainly exist in plant cell walls where they make an important impact in the transport of diverse lipophilic compounds. They have an important effect on the stress tolerance of plants by mediating plant responses to environmental stimuli and cell signal transduction pathways. Although the functions of several LTPS in different plant species have been identified, little is known about the biochemical and enzymatic activities of LTP family members in soybeans.Results: Herein, GsLTP was identified from Glycine soja using soybean gene microarray expression and screened in a salt stress expressed sequence tag (EST) library. The 369 bp open reading frame (ORF) encodes a protein of 122 amino acids. The cDNA sequence shares similarity with LTP genes in other plants such as soybean (93%) and Vigna (72%). The function of the gene was characterized in transgenic tobacco. Various physiological characteristics under different environmental stresses were investigated. We discovered that overexpression of GsLTP enhanced tolerance to drought and salt stresses.Conclusions: Our results indicate that GsLTP plays an important role in multiple abiotic signalling pathways, and provide a theoretical basis and practical gene resource for crop breeding.

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Overexpression of Brassica campestris BcICE1 gene increases abiotic stress tolerance in tobacco

Cited by 31 publications

References 22 publications

Genome-wide analysis of the bHLH gene family in Chinese jujube (Ziziphus jujuba Mill.) and wild jujube

Genome-wide analysis of the bHLH gene family in Chinese jujube (Ziziphus jujuba Mill.) and wild jujube

GsLTP, a Non-Specific Lipid Transfer Protein from Glycine soja, Enhances Drought and Salt Stress Tolerance in Transgenic Tobacco

GsLTP, a Non-Specific Lipid Transfer Protein from Glycine soja, Enhances Drought and Salt Stress Tolerance in Transgenic Tobacco

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