Overexpression of Beclin1 gene leads to reduction of telomerase activity in MDCK cells and enhances apoptosis

Taji, Fatemeh; Abdoli, Asghar; Baesi, Kazem; Sheikholeslami, Farzaneh; Kouchesfahani, Homa Mohseni

doi:10.4103/jcrt.jcrt_265_17

Cited by 2 publications

(2 citation statements)

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“…Researchers hope to increase the lifespan of these patients by reducing the complications. Some studies have reported the in vitro antiviral activity of autophagy in humans, as well as mouse neuroblastoma cell lines [31]. They also suggested that autophagy is induced by binding of the virus to Hsp90AA1 and inactivation of AKT-mTOR pathway [45], while the opposite occurs in some viruses [46].…”

Section: Discussionmentioning

confidence: 99%

“…Beclin-1 expression, which regulates ß-amyloid accumulation in the brains of mice, decreases in the early stages of Alzheimer's disease [29] Beclin-1 diminishes α-synuclein accumulation in the limbic system of Parkinson's models, increases lysosomal activation, and plays a crucial role in intracellular decadence of a-synuclein through the autophagy pathway [30]. Taji F, et al [31] found that Beclin-1 overexpression in MDCK cells diminished telomerase activity and increased apoptosis. Generally, blockade of Beclin-1 autophagy and inhibition of Fas-mediated apoptosis by γ2-herpes virus Bcl-2 homolog (vBcl-2) are important signals for viral amplifi cation [32].…”

Section: Introductionmentioning

confidence: 99%

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Development of a New Anti-Rabies Antiviral: Effective Treatment with Beclin-1 and Lifespan Prolongation

Parizad,

Abdoli,

Mostafavi

et al. 2023

J Biomed Res Environ Sci

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Autophagy is a cellular mechanism that exerts antiviral effects in some virus-infected cells, while it can have opposite effects on some other cells. Rabies is an infectious disease caused by negative-stranded RNA virus. After the street Rabies Virus (RABV), the vector containing Beclin-1 were injected into the brains of mice. They were sacrificed to collect the samples. Next, the focus forming assay, TUNEL assay and q PCR were performed. The expression of MAP1LC3B, ATG5, and BECN1 genes and the relative mRNA expression of autophagy-related genes increased after in vivo transfection of the pIRES2-EGFP-Beclin-1 vector in test groups (p < 0.01). The number of viruses decreased in the brains of test group compared to the controls. Survival significantly increased in mice treated with the Beclin-1 containing vector compared to controls. The TUNEL assay did not indicate apoptosis. LC3 was notably expressed in the brain samples that were previously transfected with the pIRES-EGFP-Beclin-1 vector.

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Section: Discussionmentioning

confidence: 99%