Overexpression of BBX18 Promotes Thermomorphogenesis Through the PRR5-PIF4 Pathway

Hwang, Geonhee; Park, Jeeyoon; Kim, Soohwan; Park, Jeonghyang; Seo, Dain; Oh, Eunkyoo

doi:10.3389/fpls.2021.782352

Cited by 11 publications

(13 citation statements)

References 48 publications

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“…Instead, the ZTL‐PRR5 module might determine the sensitivity of hypocotyl growth to high temperatures by affecting the maximum activity of PIF4. Given that PRR5 is a negative regulator of high temperature‐mediated hypocotyl growth (Hwang et al, 2021; Zhu et al, 2016), the elevation in PRR5 level at high temperatures may play a role in preventing exaggerated high temperature responses, similar to HFR1 (Foreman et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…These factors are plant‐specific nuclear proteins that act in the clock genetic circuit and regulate the expression of clock‐output genes. Both TOC1 and PRR5 exhibit transcription repression activity and directly interact with PIF4 to inhibit its transcription activation activity, thereby suppressing PIF4‐mediated growth promotion (Hwang et al, 2021; Zhu et al, 2016). TOC1 and PRR5 also interact with other PIF members, including PIF3 and PIF7, and suppress shade avoidance responses (Zhang et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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ZTL regulates thermomorphogenesis through TOC1 and PRR5

Seo

Park

et al. 2023

Plant Cell & Environment

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Plants adapt to high temperature stresses through thermomorphogenesis, a process that includes stem elongation and hyponastic leaf growth. Thermomorphogenesis is gated by the circadian clock through two evening-expressed clock components, TIMING OF CAB EXPRESSION1 (TOC1) and PSEUDO-RESPONSE REGULA-TORS5 (PRR5). These proteins directly interact with and inhibit PHYTOCHROME INTERACTING FACTOR4 (PIF4), a basic helix-loop-helix transcription factor that promotes thermoresponsive growth. PIF4-mediated thermoresponsive growth is positively regulated by ZEITLUPE (ZTL), a central clock component, but the molecular mechanisms underlying this are poorly understood. Here, we show that ZTL regulates thermoresponsive growth through TOC1 and PRR5. Genetic analyses reveal that ZTL regulates PIF4 activity as well as PIF4 expression. In Arabidopsis thaliana, ztl mutants exhibit highly accumulated TOC1 and PRR5 and unresponsive expression of PIF4 target genes under exposure to high temperatures. Mutations in TOC1 and PRR5 restore thermoactivation of PIF4 target genes and thermoresponsive growth in ztl mutants. We also show that the molecular chaperone heat-shock protein 90 promotes thermoresponsive growth through the ZTL-TOC1/PRR5 signaling module. Further, we show that ZTL protein stability is increased at high temperatures. Taken together, our results demonstrate that ZTL-mediated degradation of TOC1 and PRR5 enhances the sensitivity of hypocotyl growth to high temperatures.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

ZTL regulates thermomorphogenesis through TOC1 and PRR5

Seo

Park

et al. 2023

Plant Cell & Environment

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“…It has reported that BBX18 and BBX23 interact with ELF3 to positively regulate thermomorphogenesis which dependent on COP1 and PIF4 (Ding et al 2018). In addition, BBX18 interacts with PRR5 to alleviate the PRR5-mediated suppression of PIF4 activity, concomitantly enhancing the thermoresponsive hypocotyl growth in a ELF3 independent pathway (Hwang et al 2021). BBX20, which is transcriptionally repressed by BZR1, promotes photomorphogenesis and represses brassinosteriod signaling pathway (Fan et al 2012).…”

Section: Bbxs Act As Transcriptional Regulatorsmentioning

confidence: 99%

“…Thereby, BBX18 and BBX23 positively regulate thermomophogenic development in a ELF3 dependent manner (Ding et al 2018). However, BBX18 is also documented to associate with PRR5 to alleviate the PRR5-mediated suppression of PIF4 activity which strengths the thermoresponsive hypocotyl growth in a ELF3 independent pathway (Hwang et al 2021). Thus, plants employ BBX18 to adapt to elevated ambient temperature both in ELF3 dependent and independent pathway.…”

Section: Functions Of Bbxs In Abiotic Stress Responsementioning

confidence: 99%

Multi-layered roles of BBX proteins in plant growth and development

et al. 2023

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Light and phytohormone are external and internal cues that regulate plant growth and development throughout their life cycle. BBXs (B-box domain proteins) are a group of zinc finger proteins that not only directly govern the transcription of target genes but also associate with other factors to create a meticulous regulatory network to precisely regulate numerous aspects of growth and developmental processes in plants. Recent studies demonstrate that BBXs play pivotal roles in light-controlled plant growth and development. Besides, BBXs have been documented to regulate phytohormone-mediated physiological procedures. In this review, we summarize and highlight the multi-faced role of BBXs, with a focus in photomorphogenesis, photoperiodic flowering, shade avoidance, abiotic stress, and phytohormone-mediated growth and development in plant.

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“…However, it is possible to genetically alter soybeans to reduce this photoperiod sensitivity, creating strains that can be grown in a wide range of locations and agricultural settings, to increase yields. Core genes in the light signaling pathway, such as Suppressor of phytochrome A (SPA), Blue light inhibitors of cryptochromes (BIC) and Pseudoresponse regulator 5 (PRR5), sense changes in the light environment, which in turn affects plant growth and development ( Wang et al., 2017 ; Hwang et al., 2021 ; Ponnu and Hoecker, 2021 ). For example, GmBICs regulate soybean stem elongation under low levels of blue light ( Mu et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

N6-methyladenosine mRNA methylation is important for the light response in soybean

Zhang

et al. 2023

Front. Plant Sci.

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N6-methyladenosine (m6A) modification of messenger RNA (mRNA) is the most prevalent and abundant modification in eukaryotic mRNA and posttranscriptionally modulates the transcriptome at almost all stages of mRNA metabolism. In plants, m6A is crucial for embryonic-phase growth, flowering time control, microspore generation and fruit maturation. However, the role of m6A in plant responses to light, the most important environmental stimulus, remains unexplored. Here, we profile the m6A transcriptome of Williams 82, a soybean cultivar, and reveal that m6A is highly conserved and plays an important role in the response to light stimuli in soybean. Similar to the case in Arabidopsis, m6A in soybean is enriched not only around the stop codon and within the 3’UTR but also around the start codon. Moreover, genes with methylation occurring in the 3’UTR have higher expression levels and are more prone to alternative splicing. The core genes in the light signaling pathway, GmSPA1a, GmPRR5e and GmBIC2b, undergo changes in methylation modification and transcription levels in response to light. KEGG pathway analysis revealed that differentially expressed genes with differential m6A peaks were involved in the “photosynthesis” and “circadian rhythm” pathways. Our results highlight the important role played by epitranscriptomic mRNA methylation in the light response in soybean and provide a solid basis for determining the functional role of light on RNA m6A modification in this plant.

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Overexpression of BBX18 Promotes Thermomorphogenesis Through the PRR5-PIF4 Pathway

Cited by 11 publications

References 48 publications

ZTL regulates thermomorphogenesis through TOC1 and PRR5

ZTL regulates thermomorphogenesis through TOC1 and PRR5

Multi-layered roles of BBX proteins in plant growth and development

N6-methyladenosine mRNA methylation is important for the light response in soybean

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