Overexpression of bacterial γ‐glutamylcysteine synthetase mediates changes in cadmium influx, allocation and detoxification in poplar

He, Jun; Li, Hong; Ma, Chaofeng; Zhang, Yanli; Polle, Andrea; Rennenberg, Heinz; Cheng, Xingqi; Luo, Zhibin

doi:10.1111/nph.13013

Cited by 210 publications

(113 citation statements)

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“…He et al (2013b) indicated the activity of antioxidant enzymes (SOD, POD, CAT) in Populus Â canescens were unchanged or reduced under Cd stress. In most cases, the activity of anti-oxidative enzymes was reduced after Cd exposure which did not detoxify free Cd ions in plant cells (He et al, 2015). These results also suggest antioxidant enzymes (SOD, CAT, and POD) are not involved in Cd detoxification in neither young nor old leaves of castor cultivars.…”

Section: Antioxidant Enzymesmentioning

confidence: 86%

Subcellular cadmium distribution and antioxidant enzymatic activities in the leaves of two castor ( Ricinus communis L.) cultivars exhibit differences in Cd accumulation

Zhang

Guo

Yang

et al. 2015

Ecotoxicology and Environmental Safety

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a b s t r a c tThe aims of this study were: (1) the study of cadmium (Cd) accumulation and toxicity in different castor cultivars (Ricinus communis L.); (2) to investigate changes in antioxidant enzymatic activities and the subcellular distribution of Cd in young and old leaves from two different castor cultivars, after exposure to two different Cd concentrations, and explore the underlying mechanism of Cd detoxification focusing on antioxidant enzymes and subcellular compartmentalization. The Cd concentration, toxicity, and subcellular distribution, as well as superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) activities were measured in Zibo-3 and Zibo-9 cultivars after exposure to two different concentrations of Cd (2 mg/L and 5 mg/L) for 10 days. This research revealed Cd accumulation characteristics in castor are root4 stem4 young leaf 4old leaf. Castor tolerance was Cd dose exposure and the cultivars themselves dependent. Investigation of subcellular Cd partitioning showed that Cd accumulated mainly in the heat stable protein (HSP) and cellular debris fractions, followed by the Cd rich granule (MRG), heat denatured protein (HDP), and organelle fractions. With increasing Cd concentration in nutrient solution, the decreased detoxified fractions (BDM) and the increased Cd-sensitive fractions (MSF) in young leaves may indicate the increased Cd toxicity in castor cultivars. The BDM-Cd fractions or MSF-Cd in old leaves may be linked with Cd tolerance of different cultivars of castor. The antioxidant enzymes that govern Cd detoxification were not found to be active in leaves. Taken together, these results indicate Cd tolerance and toxicity in castor can be explained by subcellular partitioning.Crown

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Section: Antioxidant Enzymesmentioning

confidence: 86%

Subcellular cadmium distribution and antioxidant enzymatic activities in the leaves of two castor ( Ricinus communis L.) cultivars exhibit differences in Cd accumulation

Zhang

Guo

Yang

et al. 2015

Ecotoxicology and Environmental Safety

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“…The genus Populus spp. is of particular interest for phytoremediation of Cd 2+ pollution (Sell et al, 2005; Krpata et al, 2008, 2009; Kieffer et al, 2009; He et al, 2011, 2013, 2015; Ma Y. et al, 2014), due to its widespread distribution, rapid growth, and genotypic differences in response to ion-specific stress (Chen and Polle, 2010; Polle et al, 2013; Chen et al, 2014; Polle and Chen, 2015). Populus tremula (Kieffer et al, 2009) and Populus × canescens (He et al, 2011) have been recently identified as woody Cd 2+ -hyperaccumulators.…”

Section: Introductionmentioning

confidence: 99%

“…Transcript levels of the transporters involved in Cd 2+ uptake and transport have been investigated in herbaceous and woody species (Kim et al, 2006; Plaza et al, 2007; Krämer, 2010; Migeon et al, 2010; Mendoza-Cózatl et al, 2011; Lin and Aarts, 2012). In poplar plants, a variety of heavy metal transporters, such as ZRT-IRT-like proteins (ZIP2, ZIP6.2), natural resistance associated macrophage proteins (NRAMP1.1, NRAMP1.3), ATP-binding cassette transporter C1 (ABCC1), heavy metal ATPase 4 (HMA4), ATP-binding cassette transporter in mitochondria (ATM3), have been suggested to play pivotal roles in Cd 2+ transport and detoxification (Ma Y. et al, 2014; He et al, 2015). In addition to these heavy metal transporters, ion channels in the plasma membrane (PM) that are permeable to Cd 2+ contribute the Cd 2+ uptake (Li et al, 2012a; Sun et al, 2013a,b; He et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…In poplar plants, a variety of heavy metal transporters, such as ZRT-IRT-like proteins (ZIP2, ZIP6.2), natural resistance associated macrophage proteins (NRAMP1.1, NRAMP1.3), ATP-binding cassette transporter C1 (ABCC1), heavy metal ATPase 4 (HMA4), ATP-binding cassette transporter in mitochondria (ATM3), have been suggested to play pivotal roles in Cd 2+ transport and detoxification (Ma Y. et al, 2014; He et al, 2015). In addition to these heavy metal transporters, ion channels in the plasma membrane (PM) that are permeable to Cd 2+ contribute the Cd 2+ uptake (Li et al, 2012a; Sun et al, 2013a,b; He et al, 2015). High external Cd 2+ concentrations establish a large electrochemical gradient facilitating the rapid movement of Cd 2+ ions through Cd 2+ -permeable channels.…”

Section: Introductionmentioning

confidence: 99%

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Paxillus involutus-Facilitated Cd2+ Influx through Plasma Membrane Ca2+-Permeable Channels Is Stimulated by H2O2 and H+-ATPase in Ectomycorrhizal Populus × canescens under Cadmium Stress

Zhang

et al. 2017

Front. Plant Sci.

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Using a Non-invasive Micro-test Technique, flux profiles of Cd2+, Ca2+, and H+ were investigated in axenically grown cultures of two strains of Paxillus involutus (MAJ and NAU), ectomycorrhizae formed by these fungi with the woody Cd2+-hyperaccumulator, Populus × canescens, and non-mycorrhizal (NM) roots. The influx of Cd2+ increased in fungal mycelia, NM and ectomycorrhizal (EM) roots upon a 40-min shock, after short-term (ST, 24 h), or long-term (LT, 7 days) exposure to a hydroponic environment of 50 μM CdCl2. Cd2+ treatments (shock, ST, and LT) decreased Ca2+ influx in NM and EM roots but led to an enhanced influx of Ca2+ in axenically grown EM cultures of the two P. involutus isolates. The susceptibility of Cd2+ flux to typical Ca2+ channel blockers (LaCl3, GdCl3, verapamil, and TEA) in fungal mycelia and poplar roots indicated that the Cd2+ entry occurred mainly through Ca2+-permeable channels in the plasma membrane (PM). Cd2+ treatment resulted in H2O2 production. H2O2 exposure accelerated the entry of Cd2+ and Ca2+ in NM and EM roots. Cd2+ further stimulated H+ pumping activity benefiting NM and EM roots to maintain an acidic environment, which favored the entry of Cd2+ across the PM. A scavenger of reactive oxygen species, DMTU, and an inhibitor of PM H+-ATPase, orthovanadate, decreased Ca2+ and Cd2+ influx in NM and EM roots, suggesting that the entry of Cd2+ through Ca2+-permeable channels is stimulated by H2O2 and H+ pumps. Compared to NM roots, EM roots exhibited higher Cd2+-fluxes under shock, ST, and LT Cd2+ treatments. We conclude that ectomycorrhizal P. × canescens roots retained a pronounced H2O2 production and a high H+-pumping activity, which activated PM Ca2+ channels and thus facilitated a high influx of Cd2+ under Cd2+ stress.

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“…Cadmium alters a wide array of processes in growing plants, amongst which plant water relations (Haag-Kerwer et al, 1999;Perfus-Barbeoch et al, 2002;Lefevre et al, 2014), leaf gas exchanges (Baryla et al, 2001;Küpper et al, 2007;He et al, 2011;Kieffer et al, 2009;Wang et al, 2009;Parmar et al, 2013), carbohydrate and ion homeostasis (Besson-Bard et al, 2009;Wu et al, 2012;He et al, 2015), and oxidative status (He et al, 2011(He et al, , 2013a.…”

Section: Introductionmentioning

confidence: 99%

Awaiting better times: A quiescence response and adventitious root primordia formation prolong survival under cadmium stress in Tetradenia riparia (Hochst.) Codd

Bazihizina

Taiti

Serre

et al. 2016

Environmental and Experimental Botany

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A B S T R A C TPlants survive stressful conditions by employing three main strategies: escape, tolerance, and survival. Experiments were conducted to pinpoint the response strategy adopted by Tetradenia riparia to cadmium (Cd) stress by monitoring several physiological parameters and plants' ability to recover once the stress receded. Cadmium (30 and 150 mM) strongly affected shoot and root growth in a dose dependent manner, with almost complete inhibition of shoot growth, stomatal opening and CO 2 assimilation in plants exposed to 150 mM CdSO 4 . Independently of the CdSO 4 concentrations, plants excluded Cd from the aboveground tissues, with concentrations in shoots remaining around 0.1 mmol g À1 dry mass.Furthermore, Cd stress was associated with a decline in methylation of Lys4 of histone H3, likely associated with the transition from an active to a quiescent state. Surprisingly, Cd also induced the initiation of root primordia in T. riparia stems, which, once placed in a Cd-free media, quickly (24 h) developed into adventitious roots, which were likely the driving factor of the rapid resumption of leaf elongation and photosynthetic activity, which increased almost 20-fold over the 3 weeks of recovery. Therefore T. riparia ability to survive Cd stress was mediated by quiescence, which associated with an excluder strategy and stem root primordia formation, enabled rapid resumption of growth under Cd-free conditions.

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Overexpression of bacterial γ‐glutamylcysteine synthetase mediates changes in cadmium influx, allocation and detoxification in poplar

Abstract: SummaryOverexpression of bacterial c-glutamylcysteine synthetase in the cytosol of Populus tremula 9 P. alba produces higher glutathione (GSH) concentrations in leaves, thereby indicating the potential for cadmium (Cd)

Cited by 210 publications

References 83 publications

Subcellular cadmium distribution and antioxidant enzymatic activities in the leaves of two castor ( Ricinus communis L.) cultivars exhibit differences in Cd accumulation

Subcellular cadmium distribution and antioxidant enzymatic activities in the leaves of two castor ( Ricinus communis L.) cultivars exhibit differences in Cd accumulation

Paxillus involutus-Facilitated Cd2+ Influx through Plasma Membrane Ca2+-Permeable Channels Is Stimulated by H2O2 and H+-ATPase in Ectomycorrhizal Populus × canescens under Cadmium Stress

Awaiting better times: A quiescence response and adventitious root primordia formation prolong survival under cadmium stress in Tetradenia riparia (Hochst.) Codd

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