Overexpression of ATP Sulfurylase in Indian Mustard Leads to Increased Selenate Uptake, Reduction, and Tolerance1

Pilon-Smits, Elizabeth A. H.; Hwang, Seongbin; Lytle, C. Mel; Zhu, Yongliang; Tai, Jenny C.; Bravo, Rogelio C.; Chen, Yi‐Chang; Leustek, Tom; Terry, Norman

doi:10.1104/pp.119.1.123

Cited by 431 publications

(293 citation statements)

References 48 publications

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“…Root and shoot materials were harvested separately, rinsed with distilled water, and dried at 37°C for a week. Three replicates consisting of 30 to 50 seedlings were acid-digested and analyzed for Se and S by inductively coupled plasma atomic emission spectrometry as described by Pilon-Smits et al (1999).…”

Section: Quantification Of Se and S Accumulationmentioning

confidence: 99%

Cooperative Ethylene and Jasmonic Acid Signaling Regulates Selenite Resistance in Arabidopsis

2008

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Selenium (Se) is an essential element for many organisms, but excess Se is toxic. To better understand plant Se toxicity and resistance mechanisms, we compared the physiological and molecular responses of two Arabidopsis (Arabidopsis thaliana) accessions, Columbia (Col)-0 and Wassilewskija (Ws)-2, to selenite treatment. Measurement of root length Se tolerance index demonstrated a clear difference between selenite-resistant Col-0 and selenite-sensitive Ws-2. Macroarray analysis showed more pronounced selenite-induced increases in mRNA levels of ethylene-or jasmonic acid (JA)-biosynthesis and -inducible genes in Col-0 than in Ws-2. Indeed, Col-0 exhibited higher levels of ethylene and JA. The selenite-sensitive phenotype of Ws-2 was attenuated by treatment with ethylene precursor or methyl jasmonate (MeJA). Conversely, the selenite resistance of Col-0 was reduced in mutants impaired in ethylene or JA biosynthesis or signaling. Genes encoding sulfur (S) transporters and S assimilation enzymes were up-regulated by selenite in Col-0 but not Ws-2. Accordingly, Col-0 contained higher levels of total S and Se and of nonprotein thiols than Ws-2. Glutathione redox status was reduced by selenite in Ws-2 but not in Col-0. Furthermore, the generation of reactive oxygen species by selenite was higher in Col-0 than in Ws-2. Together, these results indicate that JA and ethylene play important roles in Se resistance in Arabidopsis. Reactive oxygen species may also have a signaling role, and the resistance mechanism appears to involve enhanced S uptake and reduction.

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Section: Quantification Of Se and S Accumulationmentioning

confidence: 99%

Cooperative Ethylene and Jasmonic Acid Signaling Regulates Selenite Resistance in Arabidopsis

2008

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“…An attractive solution to this problem is the genetic engineering of fast-growing Se accumulator plants, such as Indian mustard (Brassica juncea) as the efficiency of Se phytoremediation, a naturally occurring process, can be dramatically enhanced this way (4)(5)(6)(7). One strategy for genetically engineering plants for improved phytoremediation is to increase the levels of rate-limiting enzymes involved in the uptake and/or detoxification of selenium.…”

Section: Introductionmentioning

confidence: 99%

Selenium Volatiles as Proxy to the Metabolic Pathways of Selenium in Genetically Modified Brassica juncea

Kubachka

Meija

LeDuc

et al. 2007

Environ. Sci. Technol.

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Vous avez des questions? Nous pouvons vous aider. Pour communiquer directement avec un auteur, consultez la première page de la revue dans laquelle son article a été publié afin de trouver ses coordonnées. Si vous n'arrivez pas à les repérer, communiquez avec nous à PublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. Questions? Contact the NRC Publications Archive team atPublicationsArchive-ArchivesPublications@nrc-cnrc.gc.ca. If you wish to email the authors directly, please see the first page of the publication for their contact information. NRC Publications Archive Archives des publications du CNRCThis publication could be one of several versions: author's original, accepted manuscript or the publisher's version. / La version de cette publication peut être l'une des suivantes : la version prépublication de l'auteur, la version acceptée du manuscrit ou la version de l'éditeur. For the publisher's version, please access the DOI link below./ Pour consulter la version de l'éditeur, utilisez le lien DOI ci-dessous. NRC Publications Record / Notice d'Archives des publications de CNRC:http://nparc.cisti-icist.nrc-cnrc.gc.ca/eng/view/object/?id=58092c90-c1ee-4cfb-bd48-2c53f4b6d912 http://nparc.cisti-icist.nrc-cnrc.gc.ca/fra/voir/objet/?id=58092c90-c1ee-4cfb-bd48-2c53f4b6d912 Berkeley, In this study we demonstrate that the headspace selenium volatiles could be used as proxy to the metabolic pathways in the Se-accumulator plant Brassica juncea. The selenium metabolic pathways in wild type plants are compared to those of several genetically modified cultures. Complementary use of atomic and molecular mass spectrometric techniques also allowed for identification of yet unreported minor headspace Se-containing volatiles such as CH 3 SeSeSeCH 3 ,C H 3 SeSSeCH 3 , and CH 3 SeCH 2 CH 3 . By combining the information resulting from this research with the previously known information about selenium metabolism in B. juncea, it is possible that a more efficacious phytoremediation tool can be constructed. Selenium Volatiles as Proxy to the Metabolic Pathways of Selenium in Genetically Modified

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“…Plant materials were rinsed with distilled water and dried at 45°C for 48 h. Replicates were acid digested and analyzed for Se and S by inductively coupled plasma atomic emission spectrometry as described by Pilon-Smits et al (1999).…”

Section: Quantification Of Se and S Accumulationmentioning

confidence: 99%

Molecular Mechanisms of Selenium Tolerance and Hyperaccumulation in Stanleya pinnata

Freeman

Tamaoki²,

Stushnoff³

et al. 2010

Plant Physiol.

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The molecular mechanisms responsible for selenium (Se) tolerance and hyperaccumulation were studied in the Se hyperaccumulator Stanleya pinnata (Brassicaceae) by comparing it with the related secondary Se accumulator Stanleya albescens using a combination of physiological, structural, genomic, and biochemical approaches. S. pinnata accumulated 3.6-fold more Se and was tolerant to 20 mM selenate, while S. albescens suffered reduced growth, chlorosis and necrosis, impaired photosynthesis, and high levels of reactive oxygen species. Levels of ascorbic acid, glutathione, total sulfur, and nonprotein thiols were higher in S. pinnata, suggesting that Se tolerance may in part be due to increased antioxidants and up-regulated sulfur assimilation. S. pinnata had higher selenocysteine methyltransferase protein levels and, judged from liquid chromatography-mass spectrometry, mainly accumulated the free amino acid methylselenocysteine, while S. albescens accumulated mainly the free amino acid selenocystathionine. S. albescens leaf x-ray absorption near-edge structure scans mainly detected a carbon-Se-carbon compound (presumably selenocystathionine) in addition to some selenocysteine and selenate. Thus, S. albescens may accumulate more toxic forms of Se in its leaves than S. pinnata. The species also showed different leaf Se sequestration patterns: while S. albescens showed a diffuse pattern, S. pinnata sequestered Se in localized epidermal cell clusters along leaf margins and tips, concentrated inside of epidermal cells. Transcript analyses of S. pinnata showed a constitutively higher expression of genes involved in sulfur assimilation, antioxidant activities, defense, and response to (methyl)jasmonic acid, salicylic acid, or ethylene. The levels of some of these hormones were constitutively elevated in S. pinnata compared with S. albescens, and leaf Se accumulation was slightly enhanced in both species when these hormones were supplied. Thus, defense-related phytohormones may play an important signaling role in the Se hyperaccumulation of S. pinnata, perhaps by constitutively up-regulating sulfur/Se assimilation followed by methylation of selenocysteine and the targeted sequestration of methylselenocysteine.

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Overexpression of ATP Sulfurylase in Indian Mustard Leads to Increased Selenate Uptake, Reduction, and Tolerance1

Cited by 431 publications

References 48 publications

Cooperative Ethylene and Jasmonic Acid Signaling Regulates Selenite Resistance in Arabidopsis

Cooperative Ethylene and Jasmonic Acid Signaling Regulates Selenite Resistance in Arabidopsis

Selenium Volatiles as Proxy to the Metabolic Pathways of Selenium in Genetically Modified Brassica juncea

Molecular Mechanisms of Selenium Tolerance and Hyperaccumulation in Stanleya pinnata

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