Overexpression of ATHG1/AHL23 and ATPG3/AHL20, Arabidopsis AT-hook motif nuclear-localized genes, confers salt tolerance in transgenic Zoysia japonica

Jeong, Hana; Sun, Hui; Zuo, Zhi-Fang; Lee, Dong Hee; Song, Pill‐Soon; Kang, Hong‐Gyu; Lee, Hyo-Yeon

doi:10.1007/s11816-020-00606-9

Cited by 12 publications

(6 citation statements)

References 63 publications

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“… Ma et al (2019) identified a putative negative regulator of cotton plant height near one of the two QTL (HATH13.1) that co-localized with the plant height QTL from Ma et al (2019) . Near the second QTL detected in our study in the co-localized region, we identified an AT hook DNA binding motif like gene, reported to enhance plant height upon overexpression in Zoysia japonica ( Jeong et al, 2020 ) and reduced plant height in a CRISPR/Cas9 mutant of sihmg3 (protein that contains 4 AT hook repeats) in tomato ( Li et al, 2022 ).…”

Section: Discussionmentioning

confidence: 70%

Identification of small effect quantitative trait loci of plant architectural, flowering, and early maturity traits in reciprocal interspecific introgression population in cotton

Chandnani

Kim²,

Patel³

et al. 2022

Front. Plant Sci.

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Plant architecture, flowering time and maturity traits are important determinants of yield and fiber quality of cotton. Genetic dissection of loci determining these yield and quality components is complicated by numerous loci with alleles conferring small differences. Therefore, mapping populations segregating for smaller numbers and sizes of introgressed segments is expected to facilitate dissection of these complex quantitative traits. At an advanced stage in the development of reciprocal advanced backcross populations from crosses between elite Gossypium hirsutum cultivar ‘Acala Maxxa’ (GH) and G. barbadense ‘Pima S6’ (GB), we undertook mapping of plant architectural traits, flowering time and maturity. A total of 284 BC4F1 and BC4F2 progeny rows, 120 in GH and 164 in GB background, were evaluated for phenotype, with only 4 and 3 (of 7) traits showing significant differences among progenies. Genotyping by sequencing yielded 3,186 and 3,026 SNPs, respectively, that revealed a total of 27 QTLs in GH background and 22 in GB, for plant height, days to flowering, residual flowering at maturity and maturity. More than of 90% QTLs identified in both backgrounds had small effects (%PV < 10), supporting the merit of this population structure to reduce background noise and small effect QTLs. Germplasm developed in this study may serve as potential pre-breeding material to develop improved cotton cultivars.

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Section: Discussionmentioning

confidence: 70%

Identification of small effect quantitative trait loci of plant architectural, flowering, and early maturity traits in reciprocal interspecific introgression population in cotton

Chandnani

Kim²,

Patel³

et al. 2022

Front. Plant Sci.

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“…1). In zoysiagrass transgenesis, the CaMV 35S or maize ubiquitin promoters have been used to ensure strong expression of genes (Jeong et al 2020;Kim et al 2020;Zuo et al 2020). Here, we used the ubiquitin1 promoter to drive expression casettes in our vectors, since the luciferase activity driven by the control of the maize ubiquitin 1 promoter were found to be relatively higher in zoysiagrass protoplasts than that driven by the control of the 35S promoter (Figs.…”

Section: Discussionmentioning

confidence: 99%

“…To test the feasibility of the promoter assay in zoysiagrass protoplasts, we compared the promoter activity of CaMV 35S and ZmUbi1 promoters using firefly luciferase (LUC). Although CaMV 35S and ZmUbi promoters have been widely used for constitutive expression in dicot and monocot plants, respectively, but both were used for overexpression in zoysiagrass studies (Jeong et al 2020;Kim et al 2020). Therefore, we transfected zoysiagrass protoplasts with the constructs expressing the luciferase gene under the control of minimal (Min pro-LUC), ZmUbi1 (ZmUbi1 pro-LUC), and CaMV 35S (35S pro-LUC).…”

Section: Promoter Assay Using Zoysiagrass Protoplastsmentioning

confidence: 99%

Transient gene expression system in zoysiagrass leaf mesophyll protoplasts

Kim

Doan

Lee

et al. 2021

Plant Biotechnol Rep

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Transient expression of genes in protoplasts is a versatile technique for rapid functional characterization of genes by assessing protein localization or effector-reporter responses. In addition, protoplasts have been widely used for generating transgenic or gene-edited plants in model or crop plants. Zoysiagrass (Zoysia japonica Steud.) is one of the most economically important turf plants used in many living places or natural fields, but its management is labor-intensive. Therefore, genetic manipulation using valuable genes is highly demanded in zoysiagrass. Although transient expression systems in zoysiagrass facilitates the identification of valuable zoysiagrass genes, transient expression use in the zoysiagrass protoplasts is yet to be reported. Here we describe the methodology and feasibility for transient expression of genes in the zoysiagrass protoplasts isolated from green leaves. We obtained more than 70% of transfection efficiency in the zoysiagrass protoplasts using polyethylene glycolmediated transfection. Additionally, we showed the feasibility of cellular, biochemical, and molecular approaches using the zoysiagrass protoplasts transiently expressing genes of interest through fluorescent microscopic observation, immunoblot analysis, and luciferase-based promoter activity assay. Along with the genome draft of the Zoysia family, transient expression will be a valuable tool to rapidly investigate gene functions. An initial assessment of gene function using protoplasts facilitate the identification of valuable genes that can improve the economic value of the Z. japonica or its closely related species by gene manipulation.

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“…During the last decade, AHLs have been demonstrated to function in the regulation of diverse plant growth and development processes, including hypocotyl elongation (Zhao et al ., 2013), pollen wall and flower development (Yun et al ., 2012; Lou et al ., 2014), root development (Zhou et al ., 2013), petiole growth (Favero et al ., 2020), and phytohormone signaling pathways (Favero et al ., 2020). Moreover, AHL genes were found to be involved in plant responses to pathogen attack and salt and drought stresses (Zhou et al ., 2016; Howden et al ., 2017; Jeong et al ., 2020; Rayapuram et al ., 2021). Nevertheless, there is a great scarcity in understanding the biological roles and molecular mechanisms of AHL proteins in cold tolerance.…”

Section: Introductionmentioning

confidence: 99%

Two AT‐Hook proteins regulate A/NINV7 expression to modulate sucrose catabolism for cold tolerance in Poncirus trifoliata

et al. 2022

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Invertase (INV)-mediated sucrose (Suc) hydrolysis, leading to the irreversible production of glucose (Glc) and fructose (Frc), plays an essential role in abiotic stress tolerance of plants. However, the regulatory network associated with the Suc catabolism in response to cold environment remains largely elusive.Herein, the cold-induced alkaline/neutral INV gene PtrA/NINV7 of trifoliate orange (Poncirus trifoliata (L.) Raf.) was shown to function in cold tolerance via mediating the Suc hydrolysis. Meanwhile, a nuclear matrix-associated region containing A/T-rich sequences within its promoter was indispensable for the cold induction of PtrA/NINV7.Two AT-Hook Motif Containing Nuclear Localized (AHL) proteins, PtrAHL14 and PtrAHL17, were identified as upstream transcriptional activators of PtrA/NINV7 by interacting with the A/T-rich motifs. PtrAHL14 and PtrAHL17 function positively in the cold tolerance by modulating PtrA/NINV7-mediated Suc catabolism. Furthermore, both PtrAHL14 and PtrAHL17 could form homo-and heterodimers between each other, and interacted with two histone acetyltransferases (HATs), GCN5 and TAF1, leading to elevated histone3 acetylation level under the cold stress.Taken together, our findings unraveled a new cold-responsive signaling module (AHL14/17-HATs-A/NINV7) for orchestration of Suc catabolism and cold tolerance, which shed light on the molecular mechanisms underlying Suc catabolism catalyzed by A/NINVs under cold stress.

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Overexpression of ATHG1/AHL23 and ATPG3/AHL20, Arabidopsis AT-hook motif nuclear-localized genes, confers salt tolerance in transgenic Zoysia japonica

Cited by 12 publications

References 63 publications

Identification of small effect quantitative trait loci of plant architectural, flowering, and early maturity traits in reciprocal interspecific introgression population in cotton

Identification of small effect quantitative trait loci of plant architectural, flowering, and early maturity traits in reciprocal interspecific introgression population in cotton

Transient gene expression system in zoysiagrass leaf mesophyll protoplasts

Two AT‐Hook proteins regulate A/NINV7 expression to modulate sucrose catabolism for cold tolerance in Poncirus trifoliata

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