Overexpression of Arabidopsis NLP7 improves plant growth under both nitrogen-limiting and -sufficient conditions by enhancing nitrogen and carbon assimilation

Yu, Linhui; Wu, Jie; Tang, Hui; Yang, Yuan; Wang, Shimei; Wang, Yuping; Zhu, Qi-Sheng; Li, Shigui; Xiang, Cheng‐Bin

doi:10.1038/srep27795

Cited by 117 publications

(73 citation statements)

References 64 publications

(108 reference statements)

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“…Currently, NLP7 is considered the master regulator for nitrate signaling, conveying information of a cell's nitrate status to metabolic and developmental processes (Castaings et al ., ). However, other NLPs have also been described to function in distinct developmental processes (Yu et al ., ). Given that they are expressed in almost all plant organs (Winter et al ., ; Castaings et al ., ; Chardin et al ., ), it will be interesting to uncover the functional significance of each of the NLPs in the future.…”

Section: Discussionmentioning

confidence: 97%

Nitrate acts at the Arabidopsis thaliana shoot apical meristem to regulate flowering time

et al. 2019

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Summary Optimal timing of flowering, a major determinant for crop productivity, is controlled by environmental and endogenous cues. Nutrients are known to modify flowering time; however, our understanding of how nutrients interact with the known pathways, especially at the shoot apical meristem ( SAM ), is still incomplete. Given the negative side‐effects of nitrogen fertilization, it is essential to understand its mode of action for sustainable crop production. We investigated how a moderate restriction by nitrate is integrated into the flowering network at the SAM , to which plants can adapt without stress symptoms. This condition delays flowering by decreasing expression of SUPRESSOR OF OVEREXPRESSION OF CONSTANS 1 ( SOC 1 ) at the SAM . Measurements of nitrate and the responses of nitrate‐responsive genes suggest that nitrate functions as a signal at the SAM . The transcription factors NIN ‐ LIKE PROTEIN 7 ( NLP 7) and NLP 6, which act as master regulators of nitrate signaling by binding to nitrate‐responsive elements ( NRE s), are expressed at the SAM and flowering is delayed in single and double mutants. Two upstream regulators of SOC 1 ( SQUAMOSA PROMOTER BINDING PROTEIN ‐ LIKE 3 ( SPL 3 ) and SPL 5 ) contain functional NRE s in their promoters. Our results point at a tissue‐specific, nitrate‐mediated flowering time control in Arabidopsis thaliana .

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Section: Discussionmentioning

confidence: 97%

Nitrate acts at the Arabidopsis thaliana shoot apical meristem to regulate flowering time

et al. 2019

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“…This shift in R/S ratio agrees with the study of W. japonica (Hoang et al ), where the PA‐cultivated plants developed more robust root system. However, R/S ratio reflects the source–sink interactions that modulate carbon assimilation, translocation, partitioning and storage throughout the plant and have a pivotal role in specific plant strategy (Yu et al , Chang and Zhu ). Therefore, it can be expected that the remarkably different C allocations resulting from using PM or PA nutrition will be observed in various plant species.…”

Section: Discussionmentioning

confidence: 99%

Mixotrophic in vitro cultivations: the way to go astray in plant physiology

Ševčı́ková

Lhotáková

Hamet

et al. 2019

Physiologia Plantarum

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Rate of photosynthesis and related plant carbohydrate status are crucial factors affecting plant vigor. Sugars providing carbon and energy sources serve also as important signaling molecules governing plant growth and development through a complex regulatory network. These facts are often neglected when mixotrophic cultivation of plants in vitro is used, where artificial exogenous sugar supply hinders studies of metabolism as well as sugar‐driven developmental processes. We compared the growth, selected gas‐exchange parameters and sugar metabolism characteristics in four model plants, potato (Solanum tuberosum ‘Lada’), tobacco (Nicotiana tabacum ‘Samsun’), rapeseed (Brassica napus ‘Asgard’) and strawberry (Fragaria vesca), under both photomixotrophic (PM) and photoautotrophic (PA) conditions. To ensure PA conditions, we used our improved sun caps that serve as gas and light permeable covers for cultivation vessels. We found bigger biomass accumulation, larger leaf areas, higher stomatal conductance and higher instantaneous water use efficiency and lower root sugar contents in PA plants compared to PM ones. However, for other characteristics (root biomass, root/shoot ratio, pigment contents, leaf sugar and starch levels and transpiration rates), a strong species‐dependent reactions to the exogenous sugar supply was noted, which does not allow to create a general view on the overall impact of PM nutrition under in vitro conditions.

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“…20-day-old seedlings germinated on MS agar medium were used for hydroponic culture as previous described67. The root of each tobacco seedling was wrapped in a sponge strip and inserted into a hole made in thick polystyrene foam board.…”

Section: Methodsmentioning

confidence: 99%

ATHB17 enhances stress tolerance by coordinating photosynthesis associated nuclear gene and ATSIG5 expression in response to abiotic stress

Zhao

Rong

et al. 2017

Sci Rep

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Photosynthesis is sensitive to environmental stress and must be efficiently modulated in response to abiotic stress. However, the underlying mechanisms are not well understood. Here we report that ARABIDOPSIS THALIANA HOMEOBOX 17 (ATHB17), an Arabidopsis HD-Zip transcription factor, regulated the expression of a number of photosynthesis associated nuclear genes (PhANGs) involved in the light reaction and ATSIG5 in response to abiotic stress. ATHB17 was responsive to ABA and multiple stress treatments. ATHB17-overexpressing plants displayed enhanced stress tolerance, whereas its knockout mutant was more sensitive compared to the wild type. Through RNA-seq and quantitative real-time reverse transcription PCR (qRT-PCR) analysis, we found that ATHB17 did not affect the expression of many known stress-responsive marker genes. Interestingly, we found that ATHB17 down-regulated many PhANGs and could directly modulate the expression of several PhANGs by binding to their promoters. Moreover, we identified ATSIG5, encoding a plastid sigma factor, as one of the target genes of ATHB17. Loss of ATSIG5 reduced salt tolerance while overexpression of ATSIG5 enhanced salt tolerance, similar to that of ATHB17. ATHB17 can positively modulate the expression of many plastid encoded genes (PEGs) through regulation of ATSIG5. Taken together, our results suggest that ATHB17 may play an important role in protecting plants by adjusting expression of PhANGs and PEGs in response to abiotic stresses.

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Overexpression of Arabidopsis NLP7 improves plant growth under both nitrogen-limiting and -sufficient conditions by enhancing nitrogen and carbon assimilation

Cited by 117 publications

References 64 publications

Nitrate acts at the Arabidopsis thaliana shoot apical meristem to regulate flowering time

Nitrate acts at the Arabidopsis thaliana shoot apical meristem to regulate flowering time

Mixotrophic in vitro cultivations: the way to go astray in plant physiology

ATHB17 enhances stress tolerance by coordinating photosynthesis associated nuclear gene and ATSIG5 expression in response to abiotic stress

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