Overexpression of Arabidopsis <i>ESR1</i> Induces Initiation of Shoot Regeneration

Banno, Hiroharu; Ikeda, Yoshihisa; Niu, Qi-Wen; Chua, Nam‐Hai

doi:10.1105/tpc.010234

Cited by 192 publications

(94 citation statements)

References 32 publications

(41 reference statements)

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“…Several key regulators involved in plant regeneration have also been identified from other studies, for instance, using Arabidopsis cDNA overexpression screening (Banno et al 2001), Arabidopsis temperature sensitive mutants (Ohtani and Sugiyama 2005;Tamaki et al 2009;Ohbayashi et al 2011), and quantitative trait loci analyses based on comparison of Arabidopsis accessions (Motte et al 2014). Such regulators are interconnected at multiple levels and further system-level studies of these molecular networks should accelerate our understanding of plant cell totipotency as well as building efficient tissue culture systems.…”

Section: Future Perspectivesmentioning

confidence: 96%

WIND1-based acquisition of regeneration competency in Arabidopsis and rapeseed

et al. 2015

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Callus formation and de novo organogenesis often occur in the wounded tissues of plants. Although this regenerative capacity of plant cells has been utilized for many years, molecular basis for the wound-induced acquisition of regeneration competency is yet to be elucidated. Here we find that wounding treatment is essential for shoot regeneration from roots in the conventional tissue culture of Arabidopsis thaliana. Furthermore, we show that an AP2/ERF transcription factor WOUND INDUCED DEDIFFERENTIATION1 (WIND1) plays a pivotal role for the acquisition of regeneration competency in the culture system. Ectopic expression of WIND1 can bypass both wounding and auxin pre-treatment and increase de novo shoot regeneration from root explants cultured on shoot-regeneration promoting media. In Brassica napus, activation of Arabidopsis WIND1 also greatly enhances de novo shoot regeneration, further corroborating the role of WIND1 in conferring cellular regenerative capacity. Our data also show that sequential activation of WIND1 and an embryonic regulator LEAFY COTYLEDON2 enhances generation of embryonic callus, suggesting that combining WIND1 with other transcription factors promote efficient and organ-specific regeneration. Our findings in the model plant and crop plant point to a possible way to efficiently induce callus formation and regeneration by utilizing transcription factors as a molecular switch.

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Section: Future Perspectivesmentioning

confidence: 96%

WIND1-based acquisition of regeneration competency in Arabidopsis and rapeseed

et al. 2015

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“…Several other factors have also been implicated in shoot regeneration such as ENHANCER OF SHOOT REGENERATION 1 (ESR1) and ESR2 which trigger shoot regeneration in a hormone-independent medium (Banno, Ikeda, Niu, & Chua, 2001;Ikeda, Banno, Niu, Howell, & Chua, 2006). ESR2 activates CUC1 at the transcript level to promote shoot regeneration (Ikeda et al, 2006).…”

Section: Organization Of Functional Shoot Meristem and Completion Of mentioning

confidence: 99%

De novo assembly of plant body plan: a step ahead of Deadpool

et al. 2016

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While in the movie Deadpool it is possible for a human to recreate an arm from scratch, in reality plants can even surpass that. Not only can they regenerate lost parts, but also the whole plant body can be reborn from a few existing cells. Despite the decades old realization that plant cells possess the ability to regenerate a complete shoot and root system, it is only now that the underlying mechanisms are being unraveled. De novo plant regeneration involves the initiation of regenerative mass, acquisition of the pluripotent state, reconstitution of stem cells and assembly of regulatory interactions. Recent studies have furthered our understanding on the making of a complete plant system in the absence of embryonic positional cues. We review the recent studies probing the molecular mechanisms of de novo plant regeneration in response to external inductive cues and our current knowledge of direct reprogramming of root to shoot and vice versa. We further discuss how de novo regeneration can be exploited to meet the demands of green culture industries and to serve as a general model to address the fundamental questions of regeneration across the plant kingdom.

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“…The mechanism by which CKs induce the regeneration of shoot apical meristems (SAMs) is not fully understood, but recent tissue culture research has shown renewed interest in the process in Arabidopsis (Cary et al, 2002;Che et al, 2002Che et al, , 2006Che et al, , 2007Gordon et al, 2007), while other studies have screened for genes that are involved in CK-independent regeneration of shoots (Banno et al, 2001;Daimon et al, 2003;Ikeda et al, 2006). Several key studies have taken advantage of the shoot-forming capacity of Arabidopsis root explants to assess the regenerative capacity of mutants or transgenic lines in which hormone levels or sensitivity have been altered (Andersen et al, 2008;Daimon et al, 2003;Endrizzi et al, 1996;Ikeda et al, 2006;Kakimoto, 1996;Mä hö nen et al, 2006;Ozawa et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Pluripotency of Arabidopsis xylem pericycle underlies shoot regeneration from root and hypocotyl explants grown in vitro

Atta¹,

Laurens²,

et al. 2009

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SummaryWe have established a detailed framework for the process of shoot regeneration from Arabidopsis root and hypocotyl explants grown in vitro. Using transgenic plant lines in which the GUS or GFP genes were fused to promoters of developmental genes (WUS, CLV1, CLV3, STM, CUC1, PLT1, RCH1, QC25), or to promoters of genes encoding indicators of the auxin response (DR5) or transport (PIN1), cytokinin (CK) response (ARR5) or synthesis (IPT5), or mitotic activity (CYCB1), we showed that regenerated shoots originated directly or indirectly from the pericycle cells adjacent to xylem poles. In addition, shoot regeneration appeared to be partly similar to the formation of lateral root meristems (LRMs). During pre-culture on a 2, 4-dichlorophenoxyacetic acid (2, 4-D)-rich callus-inducing medium (CIM), xylem pericycle reactivation established outgrowths that were not true calli but had many characteristics of LRMs. Transfer to a CK-rich shoot-inducing medium (SIM) resulted in early LRM-like primordia changing to shoot meristems. Direct origin of shoots from the xylem pericycle occurred upon direct culture on CK-containing media without prior growth on CIM. Thus, it appeared that the xylem pericycle is more pluripotent than previously thought. This pluripotency was accompanied by the ability of pericycle derivatives to retain diploidy, even after several rounds of cell division. In contrast, the phloem pericycle did not display such developmental plasticity, and responded to CKs with only periclinal divisions. Such observations reinforce the view that the pericycle is an 'extended meristem' that comprises two types of cell populations. They also suggest that the founder cells for LRM initiation are not initially fully specified for this developmental pathway.

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Overexpression of Arabidopsis ESR1 Induces Initiation of Shoot Regeneration

Cited by 192 publications

References 32 publications

WIND1-based acquisition of regeneration competency in Arabidopsis and rapeseed

WIND1-based acquisition of regeneration competency in Arabidopsis and rapeseed

De novo assembly of plant body plan: a step ahead of Deadpool

Pluripotency of Arabidopsis xylem pericycle underlies shoot regeneration from root and hypocotyl explants grown in vitro

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