Overexpression of ANLN in lung adenocarcinoma is associated with metastasis

Abstract

Background ANLN has been identified as an actin‐binding protein and previous studies have suggested that ANLN is associated with actin cytoskeletal dynamics. Lung adenocarcinoma is a poor prognosis tumor. Metastasis is a very common complication and is regarded as the main cause for an unsatisfactory treatment outcome. Whether ANLN is involved in the metastasis of lung adenocarcinoma is unknown. Methods We performed immunohistochemical staining and analyzed the correlation between the expression level and pathological parameters. We tested the migration and invasion of A549 and PC9 cell after interference with ANLN expression by Transwell and scratch wound healing assays. Protein expression of E‐cadherin, vimentin and N‐cadherin were detected using Western blotting and immunofluorescence staining. The same experiment was also tested after overexpression of ANLN. Results The metastasis of patients with high expression of ANLN was significantly more than that of patients with low expression of ANLN. In vitro, after interfering with ANLN expression, E‐cadherin and vimentin expression were increased and N‐cadherin expression was decreased in A549 and PC9 cells. Migration and invasion ability of A549 and PC9 cells were decreased,vice versa. Conclusion Our study suggests that the expression of ANLN in lung adenocarcinoma is associated with metastasis of cancer cells. ANLN may be involved in the metastasis of lung adenocarcinoma by promoting epithelial mesenchymal transformation of tumor cells.

ANLN promotes carcinogenesis in oral cancer by regulating PI3K/mTOR signaling pathway

“…This data further supports that ANLN is associated with poor prognosis in cancer patients and enlarges the list of cancers affected by ANLN. Based on the public data, ANLN could promote cell proliferation and growth in cancer and increase the ability of cell migration and invasion [2][3][4][5][6][7]. Our data was consistent with the previous study and for rst time, we disclosed the secret of ANLN in oral cancer.…”

“…Besides mutations in ANLN are detected in focal segmental glomerulosclerosis, more experiments suggest that ANLN contributes to tumor progression [2]. For example, overexpression of ANLN promoted cell metastasis in lung adenocarcinoma and patients with higher ANLN expression displayed much worse prognosis [3,4]. Overexpression of ANLN was found in pancreatic cancer but reduction of ANLN by microRNA inhibited cell aggressiveness ability [5,6].…”

Expression of OPN3 in lung adenocarcinoma promotes epithelial‐mesenchymal transition and tumor metastasis

“…Immunohistochemistry was carried out as per the procedure described elsewhere . The OPN3 antibody (abcam, ab228748, USA) was used as a primary antibody.…”

“…Primer sequences for OPN3 were referenced to the sequence listed in Jiao et al . Primer sequences (for CDH1, CDH2 , Vimentin, Slug, Snail ) and the experimental procedures were used as described in Xu et al …”

“…Invasion and migration tests were performed using Corning's chamber (Corning, NY, USA). The specific experimental steps were followed as described elsewhere . Cells passing through the basement membrane of the upper chamber were stained with a three‐step staining kit (ThermoFisher Scientific, USA), and statistical analysis of four random field counts was then performed under a 200x microscope.…”