Overexpression of an SKn-dehydrin gene from Eucalyptus globulus and Eucalyptus nitens enhances tolerance to freezing stress in Arabidopsis

Aguayo, Paula; Sanhueza, Javiera; Noriega, Felipe; Ochoa, Margaret; Le-Feuvre, Regis; Navarrete, Darío; Fernández, M; Valenzuela, Sofía

doi:10.1007/s00468-016-1410-9

Cited by 13 publications

(7 citation statements)

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“…Wheat WZY2 can act as a protectant to increase Escherichia coli viability, protect lactate dehydrogenase (LDH) activity and inhibit protein aggregation during temperature variation including heat and cold (Yang et al 2015 ). A number of studies have shown that the cold stress tolerance was enhanced by overexpression of different DHNs in various plant species, such as Arabidopsis (Aguayo et al 2016 ; Ochoa-Alfaro et al 2012 ; Peng et al 2008 ), tobacco (Guo et al 2017 ; Hill et al 2016 ; Liu et al 2014 ; Xing et al 2011 ), strawberry (Houde et al 2004 ), cucumber (Yin et al 2006 ), and tomato (Liu et al 2015 ), suggesting their possible functions in cold stress. Besides, loss of function of some DHN genes can also decrease the cold stress tolerance of transgenic plants.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of CsLEA11, a Y3SK2-type dehydrin gene from cucumber (Cucumis sativus), enhances tolerance to heat and cold in Escherichia coli

Zhou

et al. 2017

AMB Expr

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As the group II LEA (late embryogenesis abundant) proteins, dehydrins (DHNs) play an important role in plant growth and development, as well as in response to abiotic or biotic stress challenges. In this study, a DHN gene named CsLEA11 was identified and characterized from Cucumis sativus. Sequence analysis of CsLEA11 showed that it is a Y3SK2-type DHN protein rich in hydrophilic amino acids. Expression analyses revealed that the transcription of CsLEA11 could be significantly induced by heat and cold stress. The recombinant plasmid was transformed into Escherichia coli BL21 and isopropy-β-d-thiogalactoside (IPTG) was used to induce recombinant E. coli to express CsLEA11 gene. Overexpression of CsLEA11 in E. coli enhanced cell viability and conferred tolerance to heat and cold stress. Furthermore, CsLEA11 protein could protect the activity of lactate dehydrogenase (LDH) under heat stress. Taken together, our data demonstrate that CsLEA11 might function in tolerance of cucumber to heat and cold stress.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-017-0483-1) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Overexpression of CsLEA11, a Y3SK2-type dehydrin gene from cucumber (Cucumis sativus), enhances tolerance to heat and cold in Escherichia coli

Zhou

et al. 2017

AMB Expr

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“…Previous studies have been focused on analyses of the putative promoters of DHN genes (Zhu et al, 2014;Liu et al, 2015;Aguayo et al, 2016). All of these studies showed that the expression of DHN genes is upregulated in response to multiple hormones and abiotic stresses (Zhu et al, 2014;Liu et al, 2015;Aguayo et al, 2016). Our results show that AnDHN positively improves drought tolerance through morphological and physiological changes in A. nanus.…”

Section: Andhn Improves Drought Tolerance Via Abscisic Acidmentioning

confidence: 53%

“…Although DHNs are predicted to be stress-related proteins (Nylander et al, 2001;Kosová et al, 2007;Peng et al, 2008;Kim and Nam, 2010;Shekhawat et al, 2011), the study of DHN functions in many species is still in its infancy. Previous studies have been focused on analyses of the putative promoters of DHN genes (Zhu et al, 2014;Liu et al, 2015;Aguayo et al, 2016). All of these studies showed that the expression of DHN genes is upregulated in response to multiple hormones and abiotic stresses (Zhu et al, 2014;Liu et al, 2015;Aguayo et al, 2016).…”

Section: Andhn Improves Drought Tolerance Via Abscisic Acidmentioning

confidence: 99%

AnDHN, a Dehydrin Protein From Ammopiptanthus nanus, Mitigates the Negative Effects of Drought Stress in Plants

et al. 2021

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Dehydrins (DHNs) play crucial roles in a broad spectrum of abiotic stresses in model plants. However, the evolutionary role of DHNs has not been explored, and the function of DHN proteins is largely unknown in Ammopiptanthus nanus (A. nanus), an ancient and endangered legume species from the deserts of northwestern China. In this study, we isolated a drought-response gene (c195333_g1_i1) from a drought-induced RNA-seq library of A. nanus. Evolutionary bioinformatics showed that c195333_g1_i1 is an ortholog of Arabidopsis DHN, and we renamed it AnDHN. Moreover, DHN proteins may define a class of proteins that are evolutionarily conserved in all angiosperms that have experienced a contraction during the evolution of legumes. Arabidopsis plants overexpressing AnDHN exhibited morpho-physiological changes, such as an increased germination rate, higher relative water content (RWC), higher proline (PRO) content, increased peroxidase (POD) and catalase (CAT) activities, lower contents of malondialdehyde (MDA), H2O2 and O2–, and longer root length. Our results showed that the transgenic lines had improved drought resistance with deep root system architecture, excellent water retention, increased osmotic adjustment, and enhanced reactive oxygen species (ROS) scavenging. Furthermore, the transgenic lines also had enhanced salt and cold tolerance. Our findings demonstrate that AnDHN may be a good candidate gene for improving abiotic stress tolerance in crops.Key Message: Using transcriptome analysis in Ammopiptanthus nanus, we isolated a drought-responsive gene, AnDHN, that plays a key role in enhancing abiotic stress tolerance in plants, with strong functional diversification in legumes.

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“…The over-expression of a single DHN gene generally results in an improvement in plant stress tolerance 35 – 38 . However, this is not an absolute rule since in some cases the over-expression of a DHN gene resulted in a slight or even no measureable increase in stress tolerance 39 – 41 .…”

Section: Discussionmentioning

confidence: 99%

In vivo evidence for homo- and heterodimeric interactions of Arabidopsis thaliana dehydrins AtCOR47, AtERD10, and AtRAB18

Hernández-Sánchez

Maruri-López

Graether

et al. 2017

Sci Rep

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Dehydrins (DHNs) are intrinsically disordered proteins that play central roles in plant abiotic stress responses; however, how they work remains unclear. Herein, we report the in planta subcellular localization of Arabidopsis thaliana DHNs AtCOR47, AtERD10, and AtRAB18 through GFP translational fusions. To explore the dimerization ability of the Arabidopsis acidic DHNs AtCOR47 and AtERD10, we conducted an in planta DHN binding assay using the Bimolecular Fluorescence Complementation (BiFC) technique. Our analyses revealed homodimeric interactions for AtCOR47 and AtERD10; interestingly, heterodimeric associations also occurred with these DHNs, and these interactions were observed in the cytosol of tobacco cells. Furthermore, we evaluated whether Arabidopsis basic DHNs, such as AtRAB18, could also interact with itself and/or with AtCOR47 and AtERD10 in the BiFC system. Our data revealed homodimeric RAB18 complexes in the nucleus and cytosol, while heterodimeric associations between AtRAB18 and acidic DHNs occurred only in the cytosol. Finally, we demonstrated the presence of heterodimeric complexes among Arabidopsis AtCOR47, AtERD10, and AtRAB18 DHNs with their acidic ortholog the OpsDHN1 from Opuntia streptacantha; these heterodimeric interactions showed different subcellular distributions. Our results guide DHN research toward a new scenario where DHN/DHN oligomerization could be explored as a part of their molecular mechanism.

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Overexpression of an SKn-dehydrin gene from Eucalyptus globulus and Eucalyptus nitens enhances tolerance to freezing stress in Arabidopsis

Cited by 13 publications

References 64 publications

Overexpression of CsLEA11, a Y3SK2-type dehydrin gene from cucumber (Cucumis sativus), enhances tolerance to heat and cold in Escherichia coli

Overexpression of CsLEA11, a Y3SK2-type dehydrin gene from cucumber (Cucumis sativus), enhances tolerance to heat and cold in Escherichia coli

AnDHN, a Dehydrin Protein From Ammopiptanthus nanus, Mitigates the Negative Effects of Drought Stress in Plants

In vivo evidence for homo- and heterodimeric interactions of Arabidopsis thaliana dehydrins AtCOR47, AtERD10, and AtRAB18

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