Overexpression of an EIN3-binding F-box protein2-like gene caused elongated fruit shape and delayed fruit development and ripening in tomato

Guo, Xingming; Zhang, Yong; Tu, Yun; Wang, Yongzhong; Cheng, Wenjing; Yang, Yingwu

doi:10.1016/j.plantsci.2018.04.016

Cited by 34 publications

(16 citation statements)

References 48 publications

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“…In the present work, the expression of MaEBF1 was significantly induced by ETH treatment, which is similar to EBF genes in other fruits, such as SlEBF1/2/3 in tomato [17,52] and MaEBF2 in Cavendish banana fruit [53], indicating that MaEBF1 may be an important player in Fenjiao banana fruit ripening. Additionally, the expression of MaEBF1 was strongly reduced after 12 days at 7 °C, which may be closely related to the fruit ripening problem.…”

Section: Discussionsupporting

confidence: 74%

“…EIN3 can be degraded by EBF1/2 through an ubiquitin/proteasome-mediated pathway under no ethylene condition [16]. The function characterization of EBF genes has been studied in just a few fruits, including tomato [17,18], apple [19], and papaya [20]. At the onset of fruiting, the gene expression of SlEBF1/2 has been shown to increase sharply, suggesting that SlEBF genes may be pivotal components in regulating ethylene signal responses during tomato fruit ripening.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, the senescence and ripening of tomato were accelerated by silencing the SlEBF1/2 gene [18]. Tomato fruits that were over-expressing SlEBF2-like had an elongated fruit shape and a postponed fruit ripening and development phenotype [17]. In apple, the expression of EBF1/2 increased sharply during fruit ripening and EBF1 repressed the promoter transactivation of PG1 gene by regulating the activity of EIL1/2/3 [19].…”

Section: Introductionmentioning

confidence: 99%

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The Involvement of the Banana F-Box Protein MaEBF1 in Regulating Chilling-Inhibited Starch Degradation through Interaction with a MaNAC67-Like Protein

et al. 2019

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Low-temperature storage is a common strategy for preserving and transporting vegetables and fruits. However, many fruits are hypersensitive to chilling injury, including bananas. In the present study, storage conditions of 11 °C delayed the ripening of Fenjiao (Musa ABB Pisang Awak) banana, and the pulp could be softened after ethephon treatment. Storage conditions of 7 °C prevented fruit from fully softening, and fruit contained a significantly higher starch content and lower soluble sugar content. MaEBF1, a critical gene component in the ethylene signaling pathway, was repressed during ripening after fruit had been stored for 12 days at 7 °C. The expression of a series of starch degradation-related genes and a MaNAC67-like gene were also severely repressed. Both MaEBF1 and MaNAC67-like genes were ethylene-inducible and localized in the nucleus. MaNAC67-like protein was able to physically bind to the promoter of genes associated with starch degradation, including MaBAM6, MaSEX4, and MaMEX1. Yeast two-hybrid, GST-pull down, and BiFC assays showed that MaEBF1 interacted with the MaNAC67-like protein, and their interaction further activated the promoters of MaBAM6 and MaSEX4. The current study indicates that MaNAC67-like is a direct regulator of starch degradation and potential for involvement in regulating chilling-inhibited starch degradation by interacting with the ethylene signaling components in banana fruit. The present work paves the way for further functional analysis of MaEBF1 and MaNAC67-like in banana, which will be useful for understanding the regulation of banana starch metabolism and fruit ripening.

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Section: Discussionsupporting

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Involvement of the Banana F-Box Protein MaEBF1 in Regulating Chilling-Inhibited Starch Degradation through Interaction with a MaNAC67-Like Protein

et al. 2019

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“…Transcriptome analysis of Arabidopsis mutants found several EIN3-regulated genes that were coregulated by the other transcription factors such as PIFs (light signaling) and RHD6 (root hair development) [26], which indicates co-regulation of EIL/EIN3-activated transcription by certain developmental processes and environmental conditions. In tomato, overexpression of EIN3-binding F-box protein2 gene prompted elongated fruit shape and delayed fruit development and ripening [27]. In cotton, ethylene plays a vital role in enhancing the fiber cell elongation stage by regulating the expression of tubulin, sucrose synthase, and expansin genes [28].…”

Section: Introductionmentioning

confidence: 99%

Investigation of the EIL/EIN3 Transcription Factor Gene Family Members and Their Expression Levels in the Early Stage of Cotton Fiber Development

Salih

et al. 2020

Plants

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The ethylene-insensitive3-like/ethylene-insensitive3 (EIL/EIN3) protein family can serve as a crucial factor for plant growth and development under diverse environmental conditions. EIL/EIN3 protein is a form of a localized nuclear protein with DNA-binding activity that potentially contributes to the intricate network of primary and secondary metabolic pathways of plants. In light of recent research advances, next-generation sequencing (NGS) and novel bioinformatics tools have provided significant breakthroughs in the study of the EIL/EIN3 protein family in cotton. In turn, this paved the way to identifying and characterizing the EIL/EIN3 protein family. Hence, the high-throughput, rapid, and cost-effective meta sequence analyses have led to a remarkable understanding of protein families in addition to the discovery of novel genes, enzymes, metabolites, and other biomolecules of the higher plants. Therefore, this work highlights the recent advance in the genomic-sequencing analysis of higher plants, which has provided a plethora of function profiles of the EIL/EIN3 protein family. The regulatory role and crosstalk of different metabolic pathways, which are apparently affected by these transcription factor proteins in one way or another, are also discussed. The ethylene hormone plays an important role in the regulation of reactive oxygen species in plants under various environmental stress circumstances. EIL/EIN3 proteins are the key ethylene-signaling regulators and play important roles in promoting cotton fiber developmental stages. However, the function of EIL/EIN3 during initiation and early elongation stages of cotton fiber development has not yet been fully understood. The results provided valuable information on cotton EIL/EIN3 proteins, as well as a new vision into the evolutionary relationships of this gene family in cotton species.

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“…The E3 ligase in the Ub/26S proteasome pathway is essential for recognition of target proteins for ubiquitination, and is the specificity determinant of the E3 complex for appropriate targets [3]. To date, several hundred E3 ubiquitin ligases have been identified, one of the best characterized being the SCF protein complex consisting of RBX1, SKP1, CULLIN, and F-box proteins [4, 5]. In this complex, RBX1, CULLIN1, and SKP1 are invariant, and interact together to form a core scaffold.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis and characterization of F-box gene family in Gossypium hirsutum L

Zhang¹,

Tian²,

Li³

et al. 2019

BMC Genomics

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BackgroundF-box proteins are substrate-recognition components of the Skp1-Rbx1-Cul1-F-box protein (SCF) ubiquitin ligases. By selectively targeting the key regulatory proteins or enzymes for ubiquitination and 26S proteasome mediated degradation, F-box proteins play diverse roles in plant growth/development and in the responses of plants to both environmental and endogenous signals. Studies of F-box proteins from the model plant Arabidopsis and from many additional plant species have demonstrated that they belong to a super gene family, and function across almost all aspects of the plant life cycle. However, systematic exploration of F-box family genes in the important fiber crop cotton (Gossypium hirsutum) has not been previously performed. The genome-wide analysis of the cotton F-box gene family is now possible thanks to the completion of several cotton genome sequencing projects.ResultsIn current study, we first conducted a genome-wide investigation of cotton F-box family genes by reference to the published F-box protein sequences from other plant species. 592 F-box protein encoding genes were identified in the Gossypium hirsutume acc.TM-1 genome and, subsequently, we were able to present their gene structures, chromosomal locations, syntenic relationships with their parent species. In addition, duplication modes analysis showed that cotton F-box genes were distributed to 26 chromosomes, with the maximum number of genes being detected on chromosome 5. Although the WGD (whole-genome duplication) mode seems play a dominant role during cotton F-box gene expansion process, other duplication modes including TD (tandem duplication), PD (proximal duplication), and TRD (transposed duplication) also contribute significantly to the evolutionary expansion of cotton F-box genes. Collectively, these bioinformatic analysis suggest possible evolutionary forces underlying F-box gene diversification. Additionally, we also conducted analyses of gene ontology, and expression profiles in silico, allowing identification of F-box gene members potentially involved in hormone signal transduction.ConclusionThe results of this study provide first insights into the Gossypium hirsutum F-box gene family, which lays the foundation for future studies of functionality, particularly those involving F-box protein family members that play a role in hormone signal transduction.

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Overexpression of an EIN3-binding F-box protein2-like gene caused elongated fruit shape and delayed fruit development and ripening in tomato

Cited by 34 publications

References 48 publications

The Involvement of the Banana F-Box Protein MaEBF1 in Regulating Chilling-Inhibited Starch Degradation through Interaction with a MaNAC67-Like Protein

The Involvement of the Banana F-Box Protein MaEBF1 in Regulating Chilling-Inhibited Starch Degradation through Interaction with a MaNAC67-Like Protein

Investigation of the EIL/EIN3 Transcription Factor Gene Family Members and Their Expression Levels in the Early Stage of Cotton Fiber Development

Genome-wide analysis and characterization of F-box gene family in Gossypium hirsutum L

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