Overexpression of an AP2/ERF Type Transcription Factor OsEREBP1 Confers Biotic and Abiotic Stress Tolerance in Rice

Jisha, V.; Dampanaboina, Lavanya; Vadassery, Jyothilakshmi; Mithöfer, Axel; Kappara, Saivishnupriya; Ramanan, Rajeshwari

doi:10.1371/journal.pone.0127831

Cited by 170 publications

(104 citation statements)

References 67 publications

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“…Seed-priming with MJ and PB was found to increase AO activity in drought-sensitive genotype which may improve drought tolerance ability of the genotype. On the contrary, we observed SA to decrease AO activity by 45% in the drought-sensitive genotype ( Figure 4 ), which might be acting as antagonist (Jisha et al, 2015). A positive correlation between TPC and AO activity has been reported earlier to impart abiotic stress tolerance in plants (Trust et al, 2005; Rao et al, 2013).…”

Section: Discussionmentioning

confidence: 71%

Elicitor-Induced Biochemical and Molecular Manifestations to Improve Drought Tolerance in Rice (Oryza sativa L.) through Seed-Priming

et al. 2017

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Rice (Oryza sativa L.) is one of the major grain cereals of the Indian subcontinent which face water-deficit stress for their cultivation. Seed-priming has been reported to be a useful approach to complement stress responses in plants. In the present study, seed-priming with hormonal or chemical elicitor [viz. methyl jasmonate (MJ), salicylic acid (SA), paclobutrazol (PB)] showed significant increase in total phenolic content, antioxidant activity and expression of Rice Drought-responsive (RD1 and RD2) genes (of AP2/ERF family) in contrasting rice genotypes (Nagina-22, drought-tolerant and Pusa Sugandh-5, drought-sensitive) under drought stress. However, decrease in lipid peroxidation and protein oxidation was observed not only under the stress but also under control condition in the plants raised from primed seeds. Expression analyses of RD1 and RD2 genes showed upregulated expression in the plants raised from primed seeds under drought stress. Moreover, the RD2 gene and the drought-sensitive genotype showed better response than that of the RD1 gene and the drought-tolerant genotype in combating the effects of drought stress. Among the elicitors, MJ was found to be the most effective for seed-priming, followed by PB and SA. Growth and development of the plants raised from primed seeds were found to be better under control and drought stress conditions compared to that of the plants raised from unprimed seeds under the stress. The present study suggests that seed-priming could be one of the useful approaches to be explored toward the development of simple, cost-effective and farmer-friendly technology to enhance rice yield in rainfed areas.

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Section: Discussionmentioning

confidence: 71%

Elicitor-Induced Biochemical and Molecular Manifestations to Improve Drought Tolerance in Rice (Oryza sativa L.) through Seed-Priming

et al. 2017

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“…LOXs‐activated pathways involve JA and abscisic acid signalling, thereby priming the rice plants for enhanced survival under abiotic or biotic stress conditions (Jisha et al . ). In this study, GSH2 , GSHU35 , LOX and HO1 were found to be differentially methylated, but accompanied with increased transcripts with Aza under Cd stress (Fig.…”

Section: Discussionmentioning

confidence: 97%

“…LOX encoding an LOX (EC 1.13.11.1) catalyses oxygenation of fatty acids to their hydroperoxy derivatives and is one of the key enzymes in octadecanoid pathway upstream controlling jasmonic acid (JA) biosynthesis (Gardner 1991). LOXs-activated pathways involve JA and abscisic acid signalling, thereby priming the rice plants for enhanced survival under abiotic or biotic stress conditions (Jisha et al 2015). In this study, GSH2, GSHU35, LOX and HO1 were found to be differentially methylated, but accompanied with increased transcripts with Aza under Cd stress ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Variation of DNA methylation patterns associated with gene expression in rice (Oryza sativa) exposed to cadmium

Feng

Liu

Tao

et al. 2016

Plant Cell & Environment

148

101

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We report genome-wide single-base resolution maps of methylated cytosines and transcriptome change in Cd-exposed rice. Widespread differences were identified in CG and non-CG methylation marks between Cd-exposed and Cd-free rice genomes. There are 2320 non-redundant differentially methylated regions detected in the genome. RNA sequencing revealed 2092 DNA methylation-modified genes differentially expressed under Cd exposure. More genes were found hypermethylated than those hypomethylated in CG, CHH and CHG (where H is A, C or T) contexts in upstream, gene body and downstream regions. Many of the genes were involved in stress response, metal transport and transcription factors. Most of the DNA methylation-modified genes were transcriptionally altered under Cd stress. A subset of loss of function mutants defective in DNA methylation and histone modification activities was used to identify transcript abundance of selected genes. Compared with wide type, mutation of MET1 and DRM2 resulted in general lower transcript levels of the genes under Cd stress. Transcripts of OsIRO2, OsPR1b and Os09g02214 in drm2 were significantly reduced. A commonly used DNA methylation inhibitor 5-azacytidine was employed to investigate whether DNA demethylation affected physiological consequences. 5-azacytidine provision decreased general DNA methylation levels of selected genes, but promoted growth of rice seedlings and Cd accumulation in rice plant.

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“…EREBP was reported to confer biotic and abiotic stress tolerance in rice (Jisha et al, 2015). Serine/threonine protein kinases and receptor-like serine/threonine protein kinases have been associated with the early stage of plant defense.…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptomics Atlases Reveals Different Gene Expression Pattern Related to Fusarium Wilt Disease Resistance and Susceptibility in Two Vernicia Species

et al. 2016

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Vernicia fordii (tung oil tree) is a promising industrial crop. Unfortunately, the devastating Fusarium wilt disease has caused its great losses, while its sister species (Vernicia montana) is remarkably resistant to this pathogen. However, the genetic mechanisms underlying this difference remain largely unknown. We here generated comparative transcriptomic atlases for different stages of Fusarium oxysporum infected Vernicia root. The transcriptomes of V. fordii and V. montana were assembled de novo and contained 258,430 and 245,240 non-redundant transcripts with N50 values of 1776 and 2452, respectively. A total of 44,310 pairs of putative one-to-one orthologous genes were identified in Vernicia species. Overall, the vast majority of orthologous genes shared a remarkably similar expression mode. The expression patterns of a small set of genes were further validated by quantitative real-time PCR. Moreover, 157 unigenes whose expression significantly correlated between the two species were defined, and gene set enrichment analysis indicated roles in increased defense response and in jasmonic and salicylic acid signaling responses during pathogen attack. Co-expression network analysis further identified the 17 hub unigenes, such as the serine/threonine protein kinase D6PK, leucine-rich repeat receptor-like kinase (LRR-RLK), and EREBP transcription factor, which play essential roles in plant pathogen resistance. Intriguingly, the expression of most hub genes differed significantly between V. montana and V. fordii. Based on our results, we propose a model to describe the major molecular reactions that underlie the defense responses of resistant V. montana to F. oxysporum. These data represent a crucial step toward breeding more pathogen-resistant V. fordii.

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Overexpression of an AP2/ERF Type Transcription Factor OsEREBP1 Confers Biotic and Abiotic Stress Tolerance in Rice

Cited by 170 publications

References 67 publications

Elicitor-Induced Biochemical and Molecular Manifestations to Improve Drought Tolerance in Rice (Oryza sativa L.) through Seed-Priming

Elicitor-Induced Biochemical and Molecular Manifestations to Improve Drought Tolerance in Rice (Oryza sativa L.) through Seed-Priming

Variation of DNA methylation patterns associated with gene expression in rice (Oryza sativa) exposed to cadmium

Comparative Transcriptomics Atlases Reveals Different Gene Expression Pattern Related to Fusarium Wilt Disease Resistance and Susceptibility in Two Vernicia Species

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