Overexpression of AEG-1 in renal cell carcinoma and its correlation with tumor nuclear grade and progression

Abstract

The study was aimed at detecting the expression of a newly found oncogene, astrocyte elevated gene-1 (AEG-1), in renal cell carcinoma (RCC) and its correlation with histopathologic features and the survival of patients. Real-time reverse transcription-PCR and Western blot showed markedly higher expression of AEG-1 in 8 cases of RCC tissue compared with the paired normal tissue from the same patient. The expression level of AEG-1 was also increased in four RCC cell lines in contrast with normal tubular epithelial human kidney cells 2 (HK-2) at both mRNA and protein levels. Furthermore, immunohistochemistry analysis showed highly expressed AEG-1 in 96 of 102 (94.1%) cases of paraffin-embedded archival RCC tissue. Statistical analysis showed a significant correlation of AEG-1 expression with tumor grade (P <0.001), clinical staging (P = 0.003), T classification (P = 0.003) as well as metastasis classification (P=0.032). The means for survival time of low AEG-1 expression group was 76.98m while high AEG-1expression group was 60.94m. Our results suggest that AEG-1 protein is overexpressed in RCC and plays an important role in tumor differentiation and progression. High AEG-1 expression is closely associated with poor prognosis.

Metadherin in prostate, bladder, and kidney cancer: A systematic review

“…An in vitro study by Chen et al (32) investigated the expression of MTDH in four kidney cancer cell lines; 786-0, OS-RC-1, Caki-2 and ACHN. A significantly higher level of MTDH was expressed in these four cell lines in the cytoplasm compared to the HK-2 cells as the control (32).…”

“…MTDH overexpression in primary kidney cancer has been confirmed by immunohistochemistry, RT-PCR and western blot. Two studies found that the expression of MTDH in kidney carcinoma specimens was high compared to normal tissues (32,33). An in vitro study by Chen et al (32) investigated the expression of MTDH in four kidney cancer cell lines; 786-0, OS-RC-1, Caki-2 and ACHN.…”

MTDH expression in invasive micropapillary carcinoma of the breast

“…MTDH expression is higher in breast tumors than in normal breast tissue at mRNA and protein level and there is significant association between MTDH expression and each of tumor size, clinical stage and lymph node metastasis [2] . In addition, MTDH expression is higher in renal cell carcinoma than in the paired normal tissue from the same patient and is correlated with tumor grade, clinical stage and TNM classification [5] . Song et al [35] also found that MTDH expression was markedly correlated with TNM classification and histological differentiation in colorectal carcinoma.…”

“…Published studies have suggested that MTDH expression is up-regulated in several kinds of tumors, such as breast cancer, prostate cancer, non-small cell lung cancer, squamous cell carcinoma and renal cell carcinoma et al [1][2][3][4][5] and the level of the MTDH expression is associated with tumor invasion and metastasis. Importantly, MTDH enhances the invasiveness of breast cancer cells by inducing epithelial to mesenchymal transition [6] .…”

The emerging role of astrocyte-elevated gene-1 in hepatocellular carcinoma (Review)

“…AEG-1 is ubiquitously lowly expressed in all normal tissues, with higher expression detected in the skeletal muscle and heart and in endocrine glands such as the thyroid and adrenal gland (22). AEG-1 is markedly upregulated in HCC (16,24), breast (25), gastric (26), gallbladder (27), colorectal (28), prostate (23) and renal (29) cancer, esophageal squamous cell carcinoma (ESSC) (30), non-small cell lung cancer (NSCLC) (31), pancreatic ductal adenocarcinoma (32), tongue carcinoma (33), melanoma (22), glioblastoma multiforme (GBM) (34), acute myeloid leukemia (35), neuroblastoma (36), oligodendroglioma (37) andosteosarcoma (38), cervical (39) and ovarian carcinoma (40).…”