Overexpression of ABA Receptor PYL10 Gene Confers Drought and Cold Tolerance to Indica Rice

Verma, Rakesh Kumar; Kumar, Vinjamuri Venkata Santosh; Yadav, Shashank Kumar; Pushkar, Suchitra; Rao, M. S.; Chinnusamy, Viswanathan

doi:10.3389/fpls.2019.01488

Cited by 61 publications

(44 citation statements)

References 64 publications

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“…Nagina 22. In a previous study, OsPYL10 [47] expression was found to be downregulated by PEG, NaCl and cold stresses in the roots, but was found to be upregulated by ABA in roots. In this study also similar expression of OsPYL10 was found under these treatments.…”

Section: Regulation Of Gene Expressionmentioning

confidence: 81%

“…Previous functional validation studies have shown that constitutive/stress-inducible overexpression of OsPYL2 [42], OsPYL10 [42,47,87], and OsPYL11 (=RCAR5) [44] conferred tolerance to abiotic stresses. In a previous study, expression analysis of OsPYL11 (=RCAR5) [44] under salt, PEG and ABA treatments showed that it is significantly downregulated by ABA and salt stress.…”

Section: Regulation Of Gene Expressionmentioning

confidence: 99%

“…Since the discovery of PYL family of ABA receptors, a lot of efforts have been made to unravel the function of PYL members in diverse plant species and agriculturally important crops, including Arabidopsis [15,22,28,[32][33][34][35][36][37], Artemisia annua [38], Vitis vinifera [39,40], Oryza sativa [41][42][43][44][45][46][47], Triticum aestivum [48,49], Zea mays [50,51], Solanum lycopersicum [52], Glycine max [53], Populus [54], Hevea brasiliensis [55], strawberry [56], Gossypium hirsutum [57][58][59], Brassica rapa [60] and Brachypodium distachyon [61,62]. As compared with dicotyledonous model plant Arabidopsis, signalling modules in the monocot rice are similar in type and number which proves the conserved nature of functional ABA signalling pathway across plant species [49,[63][64][65].…”

Section: Introductionmentioning

confidence: 99%

“…Despite the advancements achieved towards the comprehension of the role of PYL gene family in Arabidopsis, functional diversity and redundancy of PYLs in development and stress responsive processes in agronomically important crop like rice is relatively less investigated. In rice OsPYL2, OsPYL8, OsPYL9, OsPYL10 and OsPYL11 receptors have been functionally characterized by overexpressing the PYL genes while CISPR/Cas9 knockout mutants of PYL genes in rice has been found to moderate abiotic stress tolerance and yield [41][42][43][44][45][46][47]. Most of these works were focused in japonica rice while very less information is available in indica rice.…”

Section: Introductionmentioning

confidence: 99%

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Genome-wide identification and characterization of ABA receptor PYL gene family in rice

et al. 2020

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Background Abscisic acid (ABA), a key phytohormone that controls plant growth and stress responses, is sensed by the pyrabactin resistance 1(PYR1)/PYR1-like (PYL)/regulatory components of the ABA receptor (RCAR) family of proteins. Comprehensive information on evolution and function of PYL gene family in rice (Oryza sativa) needs further investigation. This study made detailed analysis on evolutionary relationship between PYL family members, collinearity, synteny, gene structure, protein motifs, cis-regulatory elements (CREs), SNP variations, miRNAs targeting PYLs and expression profiles in different tissues and stress responses. Results Based on sequence homology with Arabidopsis PYL proteins, we identified a total of 13 PYLs in rice (BOP clade) and maize (PACCMAD clade), while other members of BOP (wheat – each diploid genome, barley and Brachypodium) and PACCMAD (sorghum and foxtail millet) have 8-9 PYLs. The phylogenetic analysis divided PYLs into three subfamilies that are structurally and functionally conserved across species. Gene structure and motif analysis of OsPYLs revealed that members of each subfamily have similar gene and motif structure. Segmental duplication appears be the driving force for the expansion of PYLs, and the majority of the PYLs underwent evolution under purifying selection in rice. 32 unique potential miRNAs that might target PYLs were identified in rice. Thus, the predicted regulation of PYLs through miRNAs in rice is more elaborate as compared with B. napus. Further, the miRNAs identified to in this study were also regulated by stresses, which adds additional layer of regulation of PYLs. The frequency of SAPs identified was higher in indica cultivars and were predominantly located in START domain that participate in ABA binding. The promoters of most of the OsPYLs have cis-regulatory elements involved in imparting abiotic stress responsive expression. In silico and q-RT-PCR expression analyses of PYL genes revealed multifaceted role of ABARs in shaping plant development as well as abiotic stress responses. Conclusion The predicted miRNA mediated regulation of OsPYLs and stress regulated expression of all OsPYLs, at least, under one stress, lays foundation for further validation and fine tuning ABA receptors for stress tolerance without yield penalty in rice.

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Section: Regulation Of Gene Expressionmentioning

confidence: 81%

Section: Regulation Of Gene Expressionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genome-wide identification and characterization of ABA receptor PYL gene family in rice

et al. 2020

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“…The essential roles of ABA and JA in plant stress responses and stress tolerance provide important clues for the development of stress-tolerant plants, and many successful studies reported stress-tolerant crops generated by modulation of ABA or JA responses. For example, activation or overexpression of OsPYL10, an ABA receptor increased ABA responses, leading to improved tolerance to drought [8], and overexpression of OsbZIP42 or heterologous expression of ZmbZIP4 (encoding positive regulators of ABA signaling) conferred drought tolerance in rice [9,10]. Additionally, a knock-out of the JA signaling repressor OsJAZ1 increased JA sensitivity and drought tolerance; conversely, overexpression of OsJAZ1 reduced JA sensitivity and drought tolerance [11].…”

Section: Introductionmentioning

confidence: 99%

Root Development and Stress Tolerance in rice: The Key to Improving Stress Tolerance without Yield Penalties

Seo

Seomun

Choi

et al. 2020

IJMS

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Roots anchor plants and take up water and nutrients from the soil; therefore, root development strongly affects plant growth and productivity. Moreover, increasing evidence indicates that root development is deeply involved in plant tolerance to abiotic stresses such as drought and salinity. These findings suggest that modulating root growth and development provides a potentially useful approach to improve plant abiotic stress tolerance. Such targeted approaches may avoid the yield penalties that result from growth–defense trade-offs produced by global induction of defenses against abiotic stresses. This review summarizes the developmental mechanisms underlying root development and discusses recent studies about modulation of root growth and stress tolerance in rice.

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Advances and opportunities in unraveling cold‐tolerance mechanisms in the world's primary staple food crops

Jan,

Rustgi,

Barmukh

et al. 2023

The Plant Genome

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Temperatures below or above optimal growth conditions are among the major stressors affecting productivity, end‐use quality, and distribution of key staple crops including rice (Oryza sativa), wheat (Triticum aestivum), and maize (Zea mays L.). Among temperature stresses, cold stress induces cellular changes that cause oxidative stress and slowdown metabolism, limit growth, and ultimately reduce crop productivity. Perception of cold stress by plant cells leads to the activation of cold‐responsive transcription factors and downstream genes, which ultimately impart cold tolerance. The response triggered in crops to cold stress includes gene expression/suppression, the accumulation of sugars upon chilling, and signaling molecules, among others. Much of the information on the effects of cold stress on perception, signal transduction, gene expression, and plant metabolism are available in the model plant Arabidopsis but somewhat lacking in major crops. Hence, a complete understanding of the molecular mechanisms by which staple crops respond to cold stress remain largely unknown. Here, we make an effort to elaborate on the molecular mechanisms employed in response to low‐temperature stress. We summarize the effects of cold stress on the growth and development of these crops, the mechanism of cold perception, and the role of various sensors and transducers in cold signaling. We discuss the progress in cold tolerance research at the genome, transcriptome, proteome, and metabolome levels and highlight how these findings provide opportunities for designing cold‐tolerant crops for the future.

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Overexpression of ABA Receptor PYL10 Gene Confers Drought and Cold Tolerance to Indica Rice

Cited by 61 publications

References 64 publications

Genome-wide identification and characterization of ABA receptor PYL gene family in rice

Genome-wide identification and characterization of ABA receptor PYL gene family in rice

Root Development and Stress Tolerance in rice: The Key to Improving Stress Tolerance without Yield Penalties

Advances and opportunities in unraveling cold‐tolerance mechanisms in the world's primary staple food crops

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