Overexpression of AaPIF3 promotes artemisinin production in Artemisia annua

Zhang, Qiaozhuo; Wu, N; Jian, Dongqin; Jiang, Ruiqi; Yang, Chunxian; Lan, Xiaozhong; Chen, Min; Zhang, Fangyuan; Liao, Zhihua

doi:10.1016/j.indcrop.2019.111476

Cited by 20 publications

(13 citation statements)

References 39 publications

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“…Under red light, PIF1 is degraded, which stimulates the accumulation of vindoline (Liu et al, 2019). PIF3 upregulates the expression of ADS, CYP71AV1, artemisinic aldehyde Δ11( 13) reductase (DBR2), and aldehyde dehydrogenase 1 (ALDH1), and dramatically increases the content of artemisinin under light in A. annua (Zhang et al, 2019c). AaMYB15 is reported as a negative regulator of artemisinin, it binds to the promoter of AaORA and inhibits the biosynthesis of artemisinin under light in A. annua (Wu et al, 2021).…”

Section: Summary and Perspectivesmentioning

confidence: 99%

Effects of Light on Secondary Metabolite Biosynthesis in Medicinal Plants

Zhang

Zou

et al. 2021

Front. Plant Sci.

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Secondary metabolites (SMs) found in medicinal plants are one of main sources of drugs, cosmetics, and health products. With the increase in demand for these bioactive compounds, improving the content and yield of SMs in medicinal plants has become increasingly important. The content and distribution of SMs in medicinal plants are closely related to environmental factors, especially light. In recent years, artificial light sources have been used in controlled environments for the production and conservation of medicinal germplasm. Therefore, it is essential to elucidate how light affects the accumulation of SMs in different plant species. Here, we systematically summarize recent advances in our understanding of the regulatory roles of light quality, light intensity, and photoperiod in the biosynthesis of three main types of SMs (polyphenols, alkaloids, and terpenoids), and the underlying mechanisms. This article provides a detailed overview of the role of light signaling pathways in SM biosynthesis, which will further promote the application of artificial light sources in medicinal plant production.

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Section: Summary and Perspectivesmentioning

confidence: 99%

Effects of Light on Secondary Metabolite Biosynthesis in Medicinal Plants

Zhang

Zou

et al. 2021

Front. Plant Sci.

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“…So, we prepared a nootkatone biosynthesis module for working in the cytosol, the plant expression vector pcNKT, which harbored VS , VO , and hygromycin resistance gene (Hygr, as a selection marker) driven by CaMV 35S promoter (Figure ). A. annua is very rich in sesquiterpenes; for example, the artemisinin level in leaves is generally 10–20 mg/g dry weight (DW), suggesting that FPP is abundant enough for sesquiterpene biosynthesis in A. annua . − Therefore, it is not necessary to fortify FPP formation through overexpressing FPS in the cytosol. The vector pcNKT was used to genetically transform the plants of A. annua for nootkatone production in the cytosol.…”

Section: Results and Discussionmentioning

confidence: 99%

“…Engineered A. annua plants, including cNKT and pNKT lines, were established and micropropagated according to the methods developed before (Figure A–C). − Genomic PCR was used to detect the integration of transgenes into the genome of A. annua plants. The 1636-bp fragment of VO :: NOS , the 1915-bp fragment of VS :: OCS , and the 492-bp fragment specific to hygromycin resistance gene, were simultaneously amplified from positive control (pcNKT).…”

Section: Results and Discussionmentioning

confidence: 99%

“…The plant expression vectors, pcNKT and ppNKT, were respectively introduced into Agrobacterium tumefactions EHA105 to generate engineered bacterial strains, which were used for genetic transformation of A. annua . Engineered transgenic plants of A. annua were obtained according to the methods developed before. − Micropropagated transgenic plants of A. annua were transferred into pots with substrate composed of perlite: pindstrap moss: vermiculite (1:3:6), and cultured in a greenhouse under a 16/8 h light/dark photoperiod at 25 ± 1 °C. When the plants reached around 70 cm in height, their leaves were harvested and used for molecular detection and metabolite analysis.…”

Section: Methodsmentioning

confidence: 99%

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Engineering Nootkatone Biosynthesis in Artemisia annua

et al. 2021

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Nootkatone is a valuable sesquiterpene widely used in the food, fragrance, and flavor industries. Its price is very high due to its limited production in grapefruit peels or Alaska cypress heartwoods. Chemical synthesis of nootkatone uses heavy metals, highly flammable compounds, and strong oxidants, which cause severe damage to the environment. In this study, nootkatone is synthesized in Artemisia annua, using synthetic biology methods. Engineered Artemisia annua coexpressing valencene synthase (VS) and valencene oxidase (VO) in the cytosol produced nootkatone ranging from 0.89 to 8.52 μg/g fresh weight (FW). Furthermore, transgenic Artemisia annua coexpressing farnesyl diphosphate synthase (FPS), VS, and VO in plastids produced nootkatone ranging from 12.11 to 47.80 μg/g FW. These results indicated that engineering nootkatone biosynthesis in plastids was superior to that in the cytosol. Meanwhile, artemisinin production was unaltered in nootkatone-producing Artemisia annua. Our study developed a green approach for producing nootkatone in Artemisia annua with great market potential.

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“…Since 2002, Artemisinin‐based combination therapies (ACTs) are recommended to treat malaria by the World Health Organization (WHO) 45 . However, the artemisinin content in A. annua is very low, ranging from 0.01% to 1% of dry weight in leaves, 46 resulting in the disposal of about 99% of the plant. Despite contributing significantly to the field of biomedical and fabric engineering applications, 47 its waste can cause environmental problems if destinated incorrectly.…”

Section: Introductionmentioning

confidence: 99%

Eco‐friendly foams of castor oil based‐polyurethane with Artemisia residue fillers for discarded vegetable oil sorption

Maia

Zanini

Claro

et al. 2021

J of Applied Polymer Sci

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In this article, a new approach is applied to reuse Artemisia residue (AR) as filler in polyurethane (PU) foam for vegetable oil sorption for discarded cooking oil applications. The pristine PU and PU/X%AR foams (X stands for AR content of 5–20%wt/wt) were characterized by SEM, density, contact angle (CA), thermogravimetric analysis, and Fourier transform infrared spectroscopy. The influence of two experimental factors, such as contact time (30–180 s) and initial vegetable oil concentration (20–200 g/L), was investigated in vegetable oil and vegetable oil/mineral water systems. The AR loading of the foams increased the foams' density and influenced the morphological, physical, thermal, and sorption properties. The PU/20%AR sample presented the highest CA (122.5°) and the best sorption capacity and efficiency in both systems due to the small pores size and higher frequency of pores. Langmuir and Freundlich isotherm models well defined the sorption mechanisms. The Langmuir model represented the best fit of experimental data for PU/20%AR with a maximum adsorption capacity of 16.86 g/g. The PU/20%AR presented reusability of 7 cycles, conserving their hydrophobicity after the process. Therefore, AR is an innovative route as fillers in PU foams for discarded vegetable oil sorption, and the circular economy can benefit from the reuse of discarded vegetable cooking oil.

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Overexpression of AaPIF3 promotes artemisinin production in Artemisia annua

Cited by 20 publications

References 39 publications

Effects of Light on Secondary Metabolite Biosynthesis in Medicinal Plants

Effects of Light on Secondary Metabolite Biosynthesis in Medicinal Plants

Engineering Nootkatone Biosynthesis in Artemisia annua

Eco‐friendly foams of castor oil based‐polyurethane with Artemisia residue fillers for discarded vegetable oil sorption

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