Overexpression of a yeast cadmium factor 1 (YCF1) enhances heavy metal tolerance and accumulation in Brassica juncea

Bhuiyan, Mohammed Shafi Ullah; Min, Sung Ran; Jeong, Won Joong; Sultana, Sayeda; Choi, Kwan Sam; Song, Won Yong; Lee, Youngsook; Lim, Yong Pyo; Liu, Jang R.

doi:10.1007/s11240-010-9845-y

Cited by 66 publications

(25 citation statements)

References 35 publications

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“…As already discussed above, overexpression of AtATM3 in Arabidopsis led to plants exhibiting a higher cadmium tolerance (Kim et al 2007). Similar results were obtained when AtATM3 was overexpressed in Brassica juncea (Bhuiyan et al 2011b). Growth of the transgenic plants was less impaired in the presence of cadmium and lead, and a transgenic plant accumulated approximately two times more cadmium.…”

Section: Abc Transporters and Their Potential In Heavy Metal Phytoremsupporting

confidence: 81%

ABC Transporters and Heavy Metals

Song

Park

Eisenach³

et al. 2014

Signaling and Communication in Plants

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The first evidence showing that ABC transporters are involved in heavy metal resistance in eukaryotic cells has been obtained from experiments in Schizosaccharomyces pombe and Saccharomyces cerevisae, where a half-size transporter of the ABCB subclass and an ABCC-type transporter, respectively, have been shown to confer heavy metal tolerance. Biochemical studies have indicated that vacuolar ABC transporters should also play an important role in heavy metal detoxification in plants. But it was only recently that two ABCC-type transporters, AtABCC1 and AtABCC2, have been identified as major apo-phytochelatin and phytochelatin-heavy metal(oid) complex transporters. Several plasma membrane transporters have also been shown to confer heavy metal resistance. However, with the exception of STAR1, an UDP glucose exporter, which-by altering cell wall composition-confers aluminum tolerance, the substrates required to be transported to confer heavy metal resistance by these plasma membrane-localized ABC proteins are still not elucidated. A mitochondrial ABC transporter AtATM3 was shown to be required for plant growth and development. The different studies indicate that this transporter is important for the production of cytosolic iron sulfur complexes and molybdenum cofactors, prosthetic groups required for several enzymes. However, the final proof as to which substrate is transported by AtATM3 is still missing. Several laboratories took advantage of the fact that ABC transporters are involved in heavy metal tolerance to generate transgenic plants suitable for phytoremediation. The results show that overexpression of ABC proteins alone is not sufficient to produce plants that can efficiently decontaminate soils, but they indicate that this class of transporters, when combined with other transporters and enzymes involved in heavy metal transport and detoxification, may prove a good solution to produce plants that can stabilize, and in the long term clean up, soils contaminated with heavy metals.

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Section: Abc Transporters and Their Potential In Heavy Metal Phytoremsupporting

confidence: 81%

ABC Transporters and Heavy Metals

Song

Park

Eisenach³

et al. 2014

Signaling and Communication in Plants

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“…A yeast cadmium factor 1 (YCF1), a member of the ATP-binding cassette (ABC) transporters, is localised at the vacuolar membrane in Saccharomyces cerevisiae. YCF1 gene introduced to the transgenic Brassica juncea plants showed 1.3-to 1.6-fold tolerance to cadmium ions compared to wild plants (Bhuiyan et al 2011). Overexpression of BjCdR15 in transgenic Arabidopsis thaliana and Nicotiana tabacum led to the enhancement of cadmium tolerance.…”

Section: Impact Of Members Of Brassicaceae Family On Cadmiummentioning

confidence: 92%

Cadmium Accumulation by Plants of Brassicaceae Family and Its Connection with Their Primary and Secondary Metabolism

Babula

Adam

Havel

et al. 2012

The Plant Family Brassicaceae

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The mustard family -Brassicaceae -is well known as family of plants, metallophytes, which are able to accumulate wide range of heavy metals and metalloids, especially zinc and cadmium, but also nickel, thallium, chromium and selenium. Ecological importance of this process consists partially in plants themselves to survive negative environmental conditions. There are two basic different strategies, how to survive these conditions -accumulation of heavy metals in plants tissues with different intensity in individual cell types, but also organs, which is partially given by chemical composition of cell walls, and ability to synthesize special defensive -detoxification compounds rich on thiol groupsglutathione and phytochelatins, which are able to bind heavy metals and transport them to the "secure" cell compartment -vacuole. The second principle is based on ability to exclude heavy metals. Role of secondary metabolites rich on sulphur in detoxification of heavy metals is still discussed with unclear conclusions. Members of Brassicaceae family, especially genera Thlaspi and Brassica, are well-known hyperaccumulators of heavy metals with possible utilization in phytoremediation technologies. In this review chapter, mechanisms of cadmium uptake and transport and its deposition in various plant cells and tissues are discussed with respect with

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“…Many stress-related genes have been identified and characterized for their roles (Gelvin 2005;He et al 2010;Zhang et al 2010;Subramanyam et al 2011;Bhuiyan et al 2011;Li et al 2011;Movahedi et al 2012;Yarra et al 2012). The goal of our study was to investigate the potential of the salt stress tolerant candidate gene in major crop such as soybean.…”

Section: Discussionmentioning

confidence: 99%

“…The genetic transformation technique provides a powerful tool for overcoming abiotic stress of many species. (Gelvin 2005;Phang et al 2008;He et al 2010;Zhang et al 2010;Bhuiyan et al 2011;Li et al 2011;Subramanyam et al 2011;Movahedi et al 2012;Yarra et al 2012). Soybean transgenic plants with multiple functions have been developed using Agrobacterium-mediated transformation system based on the cotyledonary-node (CN) method (Hinchee et al 1988) and improved by using half-seed explants (Paz et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of AtSZF2 from Arabidopsis Showed Enhanced Tolerance to Salt Stress in Soybean

Kim¹,

Kim²,

Pak³

et al. 2017

Plant Breed. Biotech

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Plants have adapted to environmental challenges by expressing many plant genes in response to the stresses. Among those genes, CCCH zinc finger proteins are involved in abiotic and biotic stresses. Transgenic soybean plants overexpressing AtSZF2 were produced to investigate that its ectopic overexpression enhanced salt stress tolerance by Agrobacterium-mediated transformation using half-seed explants. Sixteen transgenic lines were chosen to analyze for T-DNA insertion and transcription levels, and most of them were confirmed as positive. In further analysis with Southern blot, stable transformation event and copy number were confirmed. Following high salinity stress on the detached leaf and whole plant of two transgenic lines (#4 and #6) revealed that the ectopic expression of AtSZF2 was correlated with stress tolerance in phenotype, ion leakage and chlorophyll content with statistical significance. In another test with 20% PEG treatment, similar tolerance of transgenic plants was observed with lower ion leakage and higher chlorophyll content, indicating that the damage of cell membrane was prevented in transgenic plants. Finally, expression of various abiotic stress-responding genes was detected by reverse transcriptase and quantitative real-time PCR analysis with the transgenic plants. It could be proposed that introduction of AtSZF2 resulted in the modulation of ABA/stress responsive gene expression in transgenic soybean plants and make them tolerant against salt stress. Considering soybean as a salt-sensitive crop and importance of salt stress tolerance in specific farming region, the introduction of AtSZF2 may provide an approach for crop improvement in soybean breeding.

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Overexpression of a yeast cadmium factor 1 (YCF1) enhances heavy metal tolerance and accumulation in Brassica juncea

Cited by 66 publications

References 35 publications

ABC Transporters and Heavy Metals

ABC Transporters and Heavy Metals

Cadmium Accumulation by Plants of Brassicaceae Family and Its Connection with Their Primary and Secondary Metabolism

Overexpression of AtSZF2 from Arabidopsis Showed Enhanced Tolerance to Salt Stress in Soybean

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