Overexpression of a truncated form of the MSN2 gene enhances the initial rate of ethanol production in an industrial fuel-ethanol Saccharomyces cerevisiae strain

Bücker, Augusto; Gonçalves, Davi L.; Santo, Júlio Cézar Espírito; Stambuk, Boris U.

doi:10.1186/1753-6561-8-s4-p126

Cited by 1 publication

(1 citation statement)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Overexpression of MSN2 has been shown to increase furfural tolerance [ 52 ]. Moreover, overexpression of a truncated form of MSN2 , which was suggested to either modify transcriptional activity or alter the translocation of Msn2, has been shown to increase tolerance to ethanol [ 53 , 54 ]. In our dataset, MSN4 was not significantly differentially expressed in adapting cultures, whereas MSN2 was significantly down-regulated in adapting cultures, compared to non-adapting cultures in the final sample (t4, at 41.5 h).…”

Section: Resultsmentioning

confidence: 99%

RNA sequencing reveals metabolic and regulatory changes leading to more robust fermentation performance during short-term adaptation of Saccharomyces cerevisiae to lignocellulosic inhibitors

Dijk

Rugbjerg

Nygård

et al. 2021

Biotechnol Biofuels

View full text Add to dashboard Cite

Background The limited tolerance of Saccharomyces cerevisiae to inhibitors is a major challenge in second-generation bioethanol production, and our understanding of the molecular mechanisms providing tolerance to inhibitor-rich lignocellulosic hydrolysates is incomplete. Short-term adaptation of the yeast in the presence of dilute hydrolysate can improve its robustness and productivity during subsequent fermentation. Results We utilized RNA sequencing to investigate differential gene expression in the industrial yeast strain CR01 during short-term adaptation, mimicking industrial conditions for cell propagation. In this first transcriptomic study of short-term adaption of S. cerevisiae to lignocellulosic hydrolysate, we found that cultures respond by fine-tuned up- and down-regulation of a subset of general stress response genes. Furthermore, time-resolved RNA sequencing allowed for identification of genes that were differentially expressed at 2 or more sampling points, revealing the importance of oxidative stress response, thiamin and biotin biosynthesis. furan-aldehyde reductases and specific drug:H+ antiporters, as well as the down-regulation of certain transporter genes. Conclusions These findings provide a better understanding of the molecular mechanisms governing short-term adaptation of S. cerevisiae to lignocellulosic hydrolysate, and suggest new genetic targets for improving fermentation robustness.

show abstract

Section: Resultsmentioning

confidence: 99%