Overexpression of a tomato miR171 target gene <i>Sl<scp>GRAS</scp>24</i> impacts multiple agronomical traits via regulating gibberellin and auxin homeostasis

Huang, Wei; Peng, Shiyuan; Xian, Zhiqiang; Lin, Dongbo; Hu, Guojian; Lü, Yuan; Ren, Maozhi; Li, Zhengguo

doi:10.1111/pbi.12646

Cited by 137 publications

(114 citation statements)

References 53 publications

(78 reference statements)

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“…In contrast to the involvement of GRAS24 in the downstream signaling of GA (Huang et al ), GRAS2 likely functions in the upstream biosynthesis of GA. The differential roles of GRAS2 and GRAS24 in the GA pathway further illustrate the importance of GRAS genes for the stability of the GA pathway.…”

Section: Discussionmentioning

confidence: 81%

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Silencing GRAS2 reduces fruit weight in tomato

Wang

et al. 2018

JIPB

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GRAS family transcription factors are involved in multiple biological processes in plants. Here, we report that GRAS2 plays a vital role in regulating fruit weight in tomato (Solanum lycopersicum). We establish that the expression of GRAS2 was elevated in ovaries and maintained at a constant level in fertilized ovules. Reduction of GRAS2 expression in transgenic plants reduced fruit weight through modulating ovary growth and cell size. At the metabolic level, downregulation of GRAS2 decreased activities of the gibberellic acid biosynthesis and signal transduction pathways, leading to insufficient levels of active gibberellic acid during the initial ovary development of tomato. Moreover, genotypic diversity of GRAS2 was consistent with the molecular basis of fruit weight evolution, suggesting that GRAS2 contributes to the molecular basis of the evolution of fruit weight in tomato. Collectively, these findings enhance our understanding of GRAS2 functions, in fruit development of tomato, and demonstrate a strong association between the GRAS gene family and fruit development.

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Section: Discussionmentioning

confidence: 81%

“…For instance, DELLA , a negative regulator of GA signaling, was shown to restrain fruit growth (Marti et al ). In addition, GRAS24 regulates fruit development through modulating GA and auxin signaling (Huang et al ).…”

Section: Introductionmentioning

confidence: 99%

Silencing GRAS2 reduces fruit weight in tomato

Wang

et al. 2018

JIPB

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“…8b). Interestingly, five of these miR-NAs (except miR397) have been demonstrated to be regulated by auxin signaling [35][36][37]. Moreover, a few Fig.…”

Section: Functional Analysis Of Duckweed Lncrnas Upon Salt Treatmentmentioning

confidence: 99%

Genome-wide discovery and functional prediction of salt-responsive lncRNAs in duckweed

Ding

et al. 2020

BMC Genomics

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Background: Salt significantly depresses the growth and development of the greater duckweed, Spirodela polyrhiza, a model species of floating aquatic plants. Physiological responses of this plant to salt stress have been characterized, however, the roles of long noncoding RNAs (lncRNAs) remain unknown. Results: In this work, totally 2815 novel lncRNAs were discovered in S. polyrhiza by strand-specific RNA sequencing, of which 185 (6.6%) were expressed differentially under salinity condition. Co-expression analysis indicated that the transacting lncRNAs regulated their co-expressed genes functioning in amino acid metabolism, cell-and cell wallrelated metabolism, hormone metabolism, photosynthesis, RNA transcription, secondary metabolism, and transport. In total, 42 lncRNA-mRNA pairs that might participate in cis-acting regulation were found, and these adjacent genes were involved in cell wall, cell cycle, carbon metabolism, ROS regulation, hormone metabolism, and transcription factor. In addition, the lncRNAs probably functioning as miRNA targets were also investigated. Specifically, TCONS_ 00033722, TCONS_00044328, and TCONS_00059333 were targeted by a few well-studied salt-responsive miRNAs, supporting the involvement of miRNA and lncRNA interactions in the regulation of salt stress responses. Finally, a representative network of lncRNA-miRNA-mRNA was proposed and discussed to participate in duckweed salt stress via auxin signaling. Conclusions: This study is the first report on salt-responsive lncRNAs in duckweed, and the findings will provide a solid foundation for in-depth functional characterization of duckweed lncRNAs in response to salt stress.

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“…GA is found to play important roles in many aspects of plant development such as oroan elonoation, oermination and flowerino time. It has been reported that expression of GRAS oenes in tomato showed dosedependent response to GA (Huang et al 2017). Our results demonstrated that the majority of CaGRAS oenes detected here displayed dramatic chanoes after GA treatment.…”

Section: Discussionsupporting

confidence: 73%

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Supplemental Information 1: The Gene IDs From Arabodipsis

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Plant-specific GRAS transcription factors diversely participate in the regulation of multiple biological processes including growth and development, signal cross-talking and biotic/abiotic responses. However, this gene family was not characterized detailed in pepper (Capsicum annuum L.), an economically important vegetable crop. Here, a total of 50 Ca GRAS members were identified in the pepper genome and renamed by their respective chromosomal distribution. Genomic organization revealed that most CaGRAS genes (84%) have no intron. A phylogenetic analysis was carried out using Arabidopsis thaliana to classify pepper GARS genes into at least ten subfamilies. Multiple sequence alignment showed GRAS-typical domains present in those proteins, with the members from the same phylogenetic subfamily exhibiting the similar motif composition. The presence of highly divergent N-terminus may be associated with functional specificity of each CaGRAS protein. Expression of 12 CaGRAS genes was not detected in all tissues tested, suggesting that their functions may be lost during evolution. By contrast, the rest 38 CaGRAS genes were expressed largely in several organs, showing their important roles in pepper life activities. Moreover, 21 CaGRAS genes were differentially expressed under cold, drought, salt and GA treatments, indicating that they play vital roles in response to abiotic stress in pepper. The first comprehensive analysis of GRAS gene family in the pepper genome in this study provide insights into understanding the CRAS-mediated regulation network, benefiting the genetic improvements in pepper and some other relative plants.

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Overexpression of a tomato miR171 target gene SlGRAS24 impacts multiple agronomical traits via regulating gibberellin and auxin homeostasis

Cited by 137 publications

References 53 publications

Silencing GRAS2 reduces fruit weight in tomato

Silencing GRAS2 reduces fruit weight in tomato

Genome-wide discovery and functional prediction of salt-responsive lncRNAs in duckweed

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