Overexpression of a soybean salicylic acid methyltransferase gene confers resistance to soybean cyst nematode

Lin, Jingyu; Mazarei, Mitra; Zhao, Nan; Zhu, Junwei; Zhuang, Xiaoxi; Liu, Wusheng; Pantalone, Vincent R.; Arelli, Prakash R.; Stewart, C. Neal; Chen, Feng

doi:10.1111/pbi.12108

Cited by 66 publications

(58 citation statements)

References 51 publications

(86 reference statements)

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“…Lin et al (2013) reported that a salicylic acid methyltransferase (SAMT) gene from soybean plays a role in soybean defense against soybean cyst nematode (Heterodera glycines Ichinohe). SAMT modulates the level of SA by converting salicylic acid to methyl salicylate.…”

Section: Seed Priming Experimentsmentioning

confidence: 99%

Inhibitory effects of salicylic acid on Meloidogyne javanica reproduction in tomato plants

Moslemi

Fatemy

Bernard

2016

Span. j. agric. res.

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Root-knot nematodes (Meloidogyne spp.), play a major role in loss of agricultural production. Natural substances, such as salicylic acid (SA) could possibly be involved in inducing host plant resistance against nematodes. The present study is concerned with exploring the effects of varying concentrations of SA as seed priming and soil drench on tomato growth parameters and the reproduction of the root-knot nematode Meloidogyne javanica. SA at 50 μM concentration caused only 2% of juvenile mortality under in vitro conditions. SA applied as 50 μM seed treatment caused 95% and, as a soil drench, 78% reduction in the number of egg masses that formed on tomato plants. The numbers of galls were reduced to a lesser extent. Final nematode density per gram of soil was reduced to less than 1 by the 50 μM SA seed treatment, and in other treatments decreased by between 70 and 88% compared with control plants. Our results indicate SA has potential to lower root knot nematode reproduction in tomato, and seed priming is a fairly easy method to work with.

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Section: Seed Priming Experimentsmentioning

confidence: 99%

Inhibitory effects of salicylic acid on Meloidogyne javanica reproduction in tomato plants

Moslemi

Fatemy

Bernard

2016

Span. j. agric. res.

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“…The PCR product was cloned into pET100/D-TOPO vector using the protocol recommended by the vendor (Invitrogen, Carlsbad, CA, USA). Recombinant SmSQS was expressed in E. coli following a protocol previously described (Lin et al, 2013).…”

Section: Protein Expression In Escherichia Coli and Determination Of mentioning

confidence: 99%

Volatile squalene from a nonseed plant Selaginella moellendorffii : Emission and biosynthesis

Jiang

Chen

et al. 2015

Plant Physiology and Biochemistry

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“…In our previous study, biochemical assays characterized GmSAMT1 to be a SA methyltransferase (Lin et al ., ). Overexpression of GmSAMT1 in soybean hairy roots in two susceptible backgrounds exhibited increased resistance up to 46.6% against SCN HG type 1.2.5.7 (race 2) (Lin et al ., ). In the current study, stable GmSAMT1 of transgenic soybean was produced, and homozygous T3 soybean lines with a range of transgene expression were challenged with multiple‐HG types of SCN.…”

Section: Discussionmentioning

confidence: 97%

Transgenic soybean overexpressing GmSAMT1 exhibits resistance to multiple‐HG types of soybean cyst nematode Heterodera glycines

Lin

Mazarei

Zhao

et al. 2016

Plant Biotechnology Journal

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SummarySoybean (Glycine max (L.) Merr.) salicylic acid methyl transferase (GmSAMT1) catalyses the conversion of salicylic acid to methyl salicylate. Prior results showed that when GmSAMT1 was overexpressed in transgenic soybean hairy roots, resistance is conferred against soybean cyst nematode (SCN), Heterodera glycines Ichinohe. In this study, we produced transgenic soybean overexpressing GmSAMT1 and characterized their response to various SCN races. Transgenic plants conferred a significant reduction in the development of SCN HG type 1.2.5.7 (race 2), HG type 0 (race 3) and HG type 2.5.7 (race 5). Among transgenic lines, GmSAMT1 expression in roots was positively associated with SCN resistance. In some transgenic lines, there was a significant decrease in salicylic acid titer relative to control plants. No significant seed yield differences were observed between transgenics and control soybean plants grown in one greenhouse with 22 °C day/night temperature, whereas transgenic soybean had higher yield than controls grown a warmer greenhouse (27 °C day/23 °C night) temperature. In a 1‐year field experiment in Knoxville, TN, there was no significant difference in seed yield between the transgenic and nontransgenic soybean under conditions with negligible SCN infection. We hypothesize that GmSAMT1 expression affects salicylic acid biosynthesis, which, in turn, attenuates SCN development, without negative consequences to soybean yield or other morphological traits. Thus, we conclude that GmSAMT1 overexpression confers broad resistance to multiple SCN races, which would be potentially applicable to commercial production.

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Overexpression of a soybean salicylic acid methyltransferase gene confers resistance to soybean cyst nematode

Cited by 66 publications

References 51 publications

Inhibitory effects of salicylic acid on Meloidogyne javanica reproduction in tomato plants

Inhibitory effects of salicylic acid on Meloidogyne javanica reproduction in tomato plants

Volatile squalene from a nonseed plant Selaginella moellendorffii : Emission and biosynthesis

Transgenic soybean overexpressing GmSAMT1 exhibits resistance to multiple‐HG types of soybean cyst nematode Heterodera glycines

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