Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification

Li, Guotian; Jones, Kyle C; Eudes, Aymerick; Pidatala, Venkataramana R.; Sun, Jian; Xu, Feng; Zhang, Chengcheng; Wei, Tong; Jain, Rashmi; Birdseye, Devon; Canlas, Patrick E.; Baidoo, Edward E. K.; Duong, Phat Q.; Sharma, Manoj K.; Singh, Seema; Ruan, Deling; Keasling, Jay D.; Mortimer, Jenny C.; Loqué, Dominique; Bartley, Laura E.; Scheller, Henrik Vibe; Ronald, Pamela C.

doi:10.1186/s12896-018-0464-8

Cited by 41 publications

(55 citation statements)

References 60 publications

(79 reference statements)

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“…Some engineering efforts have focused on reducing the presence of these otherwise naturally occurring inhibitors, such as cell wall-bound ferulic acid. 17 Feedstock engineering can deliver biomass tailored to the end use, which can in turn have a dramatic impact on product yields, costs, and net environmental impacts. This requires a detailed knowledge of plant cell wall biosynthesis and carbon metabolism.…”

Section: Plant Engineering For Cell Wall Optimization and In-planta Pmentioning

confidence: 99%

Approaches for More Efficient Biological Conversion of Lignocellulosic Feedstocks to Biofuels and Bioproducts

Baral

Sundström

Das

et al. 2019

ACS Sustainable Chem. Eng.

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The future bioeconomy promises drop-in or performance-advantaged biofuels and bioproducts derived from lignocellulosic biomass, substantial greenhouse gas (GHG) emissions reductions in sectors with few or no alternatives, and increased domestic energy production in countries with sufficient biomass resources. Despite the slower than anticipated pace of commercializing next-generation biofuels, the research community continues to make dramatic improvements at every stage of production, from feedstock cultivation through conversion to final products. However, the interdisciplinary nature of bioenergy research, and the need for crosscoordination among biologists, chemists, agronomists, and engineers, make coordinating and optimizing these strategies challenging. This Perspective surveys recent advancements in bioenergy crop engineering, lignocellulosic biomass deconstruction and fractionation, catabolism of biomass-derived sugars and aromatics, and biological conversion to fuels and products. We organize major research approaches into broad categories and comment on which strategies offer synergies or trade-offs in the context of four approaches to improving the economics and carbon-efficiency of advanced biofuels and bioproducts: 1) maximize sugar conversion to a single product, 2) utilize diverse carbon sources for producing a single product, 3) convert lignin to high-value products, and 4) fractionate the hydrolysate to derive maximum value from each component.

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Section: Plant Engineering For Cell Wall Optimization and In-planta Pmentioning

confidence: 99%

Approaches for More Efficient Biological Conversion of Lignocellulosic Feedstocks to Biofuels and Bioproducts

Baral

Sundström

Das

et al. 2019

ACS Sustainable Chem. Eng.

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“…The BAHD superfamily has members in various ester synthetic pathways. The functions of several BAHD genes have been reported [43][44][45]. Here, by detecting their HXXXD and DFGWG motifs using a HMMER search, we identified 717 BAHD genes from seven Rosaceae species.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide comparative analysis of the BAHD superfamily in seven Rosaceae species and expression analysis in pear (Pyrus☓ bretschneideri)

Liu

Qiao

et al. 2019

Preprint

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Background: The BAHD acyltransferase superfamily exhibits various biological roles in plants, including regulating fruit quality, catalytic synthesizing of terpene, phenolics and esters, and improving stress resistance. However, the copy numbers, expression characteristics and associations with fruit aroma formation of the BAHD genes remain unclear. Results: In total, 717 BAHD genes were obtained from the genomes of seven Rosaceae, ( Pyrus bretschneideri , Malus domestica , Prunus avium , Prunus persica , Fragaria vesca , Pyrus communis and Rubus occidentalis ). Based on the classifications in model plants, we divided the BAHD family genes into seven groups, I-a, I-b, II-a, II-b, III-a, IV and V. An intra-species synteny analysis detected 78 syntenic gene pairs among the seven Rosaceae species. Dispersed gene duplication occurred frequently in all the investigated species. Different modes of duplicated gene pairs identified in each investigated species revealed that the Ka/Ks ratios were less than one, indicating that they evolved through purifying selection. Based on the correlation analysis between the ester content and expression levels of BAHD genes at different developmental stages, we selected five genes for verification as assessed by qRT-PCR. Pbr020016.1 , Pbr019034.1 , Pbr014028.1 and Pbr029551.1 are important candidate genes involved in aroma formation during pear fruit development. Conclusion: We have thoroughly annotated the BAHD superfamily genes and performed a comprehensive comparative analysis of their colinearity, phylogenetic relationships and gene duplication patterns in seven Rosaceae species, and we also obtained four candidate genes involved in aroma synthesis in pear fruit. These results provide a theoretical basis for future studies of the specific biological functions of BAHD superfamily members and the improvement of pear fruit quality. Keywords : BAHD, pear, evolution, Rosaceae, transcriptome, volatile esters

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“…The real time PCR amplification mixture (20 μL) contained 1 μg of cDNA, 10 μL of 2× TransStart® Top Green qPCR Super Mix Kit, 0.4 μL of 50× Dye II and 4 μL of 5 μM forward and reverse primers. The relative quantification of each transcript of different genes was calculated using the 2 -ΔΔCT method, normalized to the internal control actin gene (LOC_Os03g50885) (Li et al 2018).…”

Section: Methodsmentioning

confidence: 99%

Whole-Genome Sequencing Identifies a Rice Grain Shape Mutant, gs9–1

Jiang

Chern

et al. 2019

Rice

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Background Breeding for genes controlling key agronomic traits is an important goal of rice genetic improvement. To gain insight into genes controlling grain morphology, we screened M 3 plants derived from 1,000 whole-genome sequenced (WGS) M 2 Kitaake mutants to identify lines with altered grain size. Results In this study, we isolated a mutant, named fast-neutron (FN) 60–4, which exhibits a significant reduction in grain size. We crossed FN60–4 with the parental line Kitaake and analyzed the resulting backcross population. Segregation analysis of 113 lines from the BC 2 F 2 population revealed that the mutant phenotype is controlled by a single semi-dominant locus. Mutant FN60–4 is reduced 20% in plant height and 8.8% in 1000-grain weight compared with Kitaake. FN60–4 also exhibits an 8% reduction in cell number and a 9% reduction in cell length along the vertical axis of the glume. We carried out whole-genome sequencing of DNA pools extracted from segregants with long grains or short grains, and revealed that one gene, LOC_Os09g02650, cosegregated with the grain size phenotype in the BC 1 F 2 and BC 2 F 2 populations. This mutant allele was named grain shape 9–1 ( gs9–1 ). gs9–1 carries a 3-bp deletion that affects two amino acids. This locus is a new allele of the BC12/GDD1/MTD1 gene that encodes a kinesin-like protein involved in cell-cycle progression, cellulose microfibril deposition and gibberellic acid (GA) biosynthesis. The GA biosynthesis-related gene KO2 is down-regulated in gs9–1 . The dwarf phenotype of gs9–1 can be rescued by adding exogenous GA 3 . In contrast to the phenotypes for the other alleles, the gs9–1 is less severe, consistent with the nature of the mutation, which does not disrupt the open reading frame as observed for the other alleles. Conclusions In this study, we isolated a mutant, which exhibits altered grain shape and identified the mutated gene, gs9–1 . Our study reveals that gs9–1 is a semi-dominant gene that carries a two-amino acid mutation. gs9–1 is allelic to the BC12/GDD1/MTD1 gene involved in GA biosynthesis. These results demonstrate the efficiency and convenience of cloning genes from the whole-genome sequenced Kitaake mutant population to advance investigations into genes controlling key agronomic traits in rice. Electronic supplementary material The online version of this article (10.1186/s12284-019-0308-8) co...

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Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification

Cited by 41 publications

References 60 publications

Approaches for More Efficient Biological Conversion of Lignocellulosic Feedstocks to Biofuels and Bioproducts

Approaches for More Efficient Biological Conversion of Lignocellulosic Feedstocks to Biofuels and Bioproducts

Genome-wide comparative analysis of the BAHD superfamily in seven Rosaceae species and expression analysis in pear (Pyrus☓ bretschneideri)

Whole-Genome Sequencing Identifies a Rice Grain Shape Mutant, gs9–1

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