Overexpression of a MYB Family Gene, OsMYB6, Increases Drought and Salinity Stress Tolerance in Transgenic Rice

Tang, Yuehui; Bao, Xinxin; Yu-ling, Zhi; Wu, Qian; Guo, Yaru; XuHui, Yin; Zeng, Linjuan; Li, Jia; Zhang, Jing; He, Wenjuan; Liu, Weihao; Wang, Qingwei; Jia, Chengkai; Li, Zhengkang; Liu, Kun

doi:10.3389/fpls.2019.00168

Cited by 203 publications

(116 citation statements)

References 44 publications

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“…rty expression promotes drought stress responses via ABA-regulated stomatal closure Plant drought responses involve a complex process regulated by multiple molecular and cellular pathways. The expression levels of some genes are upregulated or downregulated by exposure to abiotic stresses, and the heterologous expression of these genes can increase the tolerance of transgenic plants to drought or salt stress [48][49][50][51]. Consistent with this, our results indicated that the eheterologous expression of rty has critical effects on strawberry drought responses.…”

Section: Heterologous Expression Of Rty Increases the Drought Toleransupporting

confidence: 83%

Heterologous Expression of Arabidopsis rty Enhances Drought Tolerance in Strawberry (Fragaria × ananassa Duch.)

Yang

Raza³

et al. 2020

Preprint

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Background Strawberry ( Fragaria × ananassa Duch.) is an important fruit crop worldwide. It was particularly sensitive to drought stress because of their fibrous and shallow root systems. Mutation of Arabidopsis thaliana ROOTY ( RTY ) results in increased endogenous auxin levels and roots and shoot growth, but the effects of this gene in strawberry remain unclear. Results Here, we heterologously expressed Arabidopsis rty in strawberry plants and examined the effects of rty expression on the hormonal and physiological properties of the plants. Heterologous expression of rty induced IAA accumulation and increased the production of adventitious roots as well as trichomes on the abaxial leaf surface of the transgenic plants. Furthermore, the transgenic strawberry plants had increased ABA accumulation and stomatal closure. The transgenic strawberry plants exhibited enhanced water use efficiency and a reduced water loss rate. Additionally, peroxidase and catalase activities were significantly higher in the transgenic plants than in the untransformed controls, and the transgenic plants were more drought tolerant than the wild-type plants. Our results uncover a transgenic approaches can be used to overcome the inherent trade-off between plant growth and drought tolerance by enhancing water use efficiency and reducing water loss rate under water shortage conditions. Conclusions In this study, the rty gene improves hormone-mediated drought tolerance in transgenic strawberry. We demonstrated that the heterologous expression of rty in strawberry improved drought tolerance by promoting auxin and ABA accumulation. These phytohormones together brought about various physiological changes that improved drought tolerance via increased root production, trichome density, and stomatal closure. This study provides the basis for future genetic modifications of strawberry to improve drought tolerance.

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Section: Heterologous Expression Of Rty Increases the Drought Toleransupporting

confidence: 83%

Heterologous Expression of Arabidopsis rty Enhances Drought Tolerance in Strawberry (Fragaria × ananassa Duch.)

Yang

Raza³

et al. 2020

Preprint

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“…The R2R3-MYB TFs regulating important plant biological processes such as primary and secondary metabolism, cell fate and identity, developmental processes, and responses to biotic and abiotic stresses [65]. Tang et al [66] showed that overexpression of OsMYB6, an R2R3-MYB TF have enhanced drought tolerance and it was probably raised from increased proline content, decreased MDA content, higher CAT and SOD activities and induced expression of abiotic stress-response genes [66]. Another identified TF with high centrality measures in the DRC-TF network was dehydrationresponsive element-binding 2D-like (DREB2D-like), a member of the DREB family of AP2/EREBF superfamily.…”

Section: Gene Regulatory Networkmentioning

confidence: 99%

Transcription Factors and microRNA Genes Regulatory Network Construction Under Drought Stress in Sesame (Sesamum indicum L.)

Baghery

Kazemitabar

Dehestani

et al. 2020

Preprint

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Background: Drought is one of the most common environmental stresses affecting crops yield and quality. Sesame is an important oilseed crop that most likely faces drought during its growth due to growing in semi-arid and arid areas. Plants responses to drought controlled by regulatory mechanisms. Despite this importance, there is little information about Sesame regulatory mechanisms against drought stress. Results: 458 drought-related genes were identified using comprehensive RNA-seq data analysis of two susceptible and tolerant sesame genotypes under drought stress. These drought-responsive genes were included secondary metabolites biosynthesis-related Like F3H, sucrose biosynthesis-related like SUS2, transporters like SUC2, and protectives like LEA and HSP families. Interactions between identified genes and regulators including TFs and miRNAs were predicted using bioinformatics tools and related regulatory gene networks were constructed. Key regulators and relations of Sesame under drought stress were detected by network analysis. TFs belonged to DREB (DREB2D), MYB (MYB63), ZFP (TFIIIA), bZIP (bZIP16), bHLH (PIF1), WRKY (WRKY30) and NAC (NAC29) families were found among key regulators. mRNAs like miR399, miR169, miR156, miR5685, miR529, miR395, miR396, and miR172 also found as key drought regulators. Furthermore, a total of 117 TFs and 133 miRNAs that might be involved in drought stress were identified with this approach. Conclusions: Most of the identified TFs and almost all of the miRNAs are introduced for the first time as potential regulators of drought response in Sesame. These regulators accompany with identified drought-related genes could be valuable candidates for future studies and breeding programs on Sesame under drought stress. Keywords: Sesamum indicum, Drought stress, Regulatory networks, miRNA, Transcription Factors.

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“…For example, plant hormones, such as abscisic acid (ABA), auxin and ethylene, could function as essential endogenous signal molecules and regulate plant development and tolerance to salinity 13,14 . The WRKY and MYB transcription factors are important to the regulation of transcriptional reprogramming involved in abiotic stress responses 15,16 , which have been demonstrated to regulate the contents of chlorophyll, proline, soluble sugar as well as activities of superoxide dismutase (SOD), and catalase (CAT) under saline stress 17,18 . In response to saline stress, metabolism of unsaturated fatty acid was activated to produce various compounds, which then function as signal molecules or participate in plant defense system as antimicrobial substances [19][20][21] .…”

mentioning

confidence: 99%

Transcriptome analyses revealed molecular responses of Cynanchum auriculatum leaves to saline stress

Zhang

et al. 2020

Sci Rep

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Cynanchum auriculatum is a traditional herbal medicine in China and can grow in saline soils. However, little is known in relation to the underlying molecular mechanisms. In the present study, C. auriculatum seedlings were exposed to 3.75‰ and 7.5‰ salinity. Next, transcriptome profiles of leaves were compared. Transcriptome sequencing showed 35,593 and 58,046 differentially expressed genes (DEGs) in treatments with 3.75‰ and 7.5‰, compared with the control, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of these DEGs enriched various defense-related biological pathways, including ROS scavenging, ion transportation, lipid metabolism and plant hormone signaling. further analyses suggested that C. auriculatum up-regulated Na + /H + exchanger and V-type proton Atpase to avoid accumulation of na + . The flavonoid and phenylpropanoids biosynthesis pathways were activated, which might increase antioxidant capacity in response to saline stress. The auxin and ethylene signaling pathways were upregulated in response to saline treatments, both of which are important plant hormones. Overall, these results raised new insights to further investigate molecular mechanisms underlying resistance of C. auriculatum to saline stress.www.nature.com/scientificreports www.nature.com/scientificreports/ and V-type proton ATPase, the flavonoid and phenylpropanoids biosynthesis pathways, the auxin and ethylene signaling pathways were upregulated to improve the antioxidant capacity of C. auriculatum in response to saline treatments. These changes might facilitate resistance of C. auriculatum to saline stress. Flavonoid and phenylpropanoids appear to play roles in resistance to salt tolerance in C. auriculatum, possibly due to its pharmacodynamics.

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Overexpression of a MYB Family Gene, OsMYB6, Increases Drought and Salinity Stress Tolerance in Transgenic Rice

Cited by 203 publications

References 44 publications

Heterologous Expression of Arabidopsis rty Enhances Drought Tolerance in Strawberry (Fragaria × ananassa Duch.)

Heterologous Expression of Arabidopsis rty Enhances Drought Tolerance in Strawberry (Fragaria × ananassa Duch.)

Transcription Factors and microRNA Genes Regulatory Network Construction Under Drought Stress in Sesame (Sesamum indicum L.)

Transcriptome analyses revealed molecular responses of Cynanchum auriculatum leaves to saline stress

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