Overexpression of a constitutively active truncated form of OsCDPK1 confers disease resistance by affecting OsPR10a expression in rice

Abstract

The rice pathogenesis-related protein OsPR10a was scarcely expressed in OsCDPK1-silenced (Ri-1) rice, which was highly sensitive to pathogen infection. After inoculating the leaves with bacterial blight (Xanthomonas oryzae pv. oryzae; Xoo), we found that the expression of OsPR10a was up- and down-regulated in OEtr-1 (overexpression of the constitutively active truncated form of OsCDPK1) and Ri-1 rice plants, respectively. OsPR10a and OsCDPK1 showed corresponding expression patterns and were up-regulated in response to the jasmonic acid, salicylic acid and Xoo treatments, and OsPR1 and OsPR4 were significantly up-regulated in OEtr-1. These results suggest that OsCDPK1 may be an upstream regulator involved in rice innate immunity and conferred broad-spectrum of disease resistance. Following the Xoo inoculation, the OEtr-1 and Ri-1 seedlings showed enhanced and reduced disease resistance, respectively. The dihybrid rice Ri-1/OsPR10a-Ox not only bypassed the effect of OsCDPK1 silencing on the susceptibility to Xoo but also showed enhanced disease resistance and, consistent with Ri-1 phenotypes, increased plant height and grain size. Our results reveal that OsCDPK1 plays novel key roles in the cross-talk and mediation of the balance between stress response and development and provides a clue for improving grain yield and disease resistance simultaneously in rice.

Overexpression of MdCPK1a gene, a calcium dependent protein kinase in apple, increase tobacco cold tolerance via scavenging ROS accumulation

“…In Arabidopsis, overexpression of SiCDPK24 enhanced drought tolerance [ 29 ] . OsCDPK1 positively regulates salt and drought tolerance in rice [ 30 ] , meanwhile it acts as a positive regulator of OsPR10a participating in the defense signaling pathway [ 31 ] . Conversely, some CDPKs are negative regulators of stress response because transgenic plants overexpressing them are more sensitive to abiotic/biotic stresses.…”

Pathogenesis-related protein 10 in resistance to biotic stress: progress in elucidating functions, regulation and modes of action

“…Some molecules influenced the susceptibility of the plant to the fungal pathogen. JAZ2 (Jasmonate-ZIM-domain) impairs JA sensitivity by decreasing the level of expression of JA response genes and increasing plant susceptibility ( He et al., 2018 ). This protein interacts with PR-10.…”

“…Based on these studies, a biological model of the responses that were triggered in the plant cell involving PR-10 was designed ( Figure 6 ). SA and JA positively activate PR-10 expression, either directly or by activating CDPK1 (Calcium-Dependent Protein Kinases) ( He et al., 2018 ). PGIP1 (Polygalacturonase-Inhibiting Proteins) seemed to act by inducing the JA pathway, which consequently positively regulated PR-10 ( Wu et al., 2019 ).…”

Recent Progress in Rice Broad-Spectrum Disease Resistance

“…In addition to CRK6 and CRK10, some kinases have been identified as conferring broad-spectrum resistance to Xoo . For instance, overexpression of a constitutively activated form of calcium-dependent protein kinases OsCDPK1 confers Xoo resistance by affecting OsPR10a expression in rice [ 48 ]. OsILA1, a Raf-like MAPKKK, functions as a negative regulator and acts upstream of the OsMAPKK4–OsMAPK6 cascade against Xoo [ 49 ].…”