Overexpression of a carrot BCH gene, DcBCH1, improves tolerance to drought in Arabidopsis thaliana

Li, Tong; Liu, Jie-Xia; Deng, Yuan-Jie; Xu, Zhihong; Xiong, Ai‐Sheng

doi:10.1186/s12870-021-03236-7

Cited by 31 publications

(9 citation statements)

References 71 publications

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“…6d ). This was consistent with the recently reported reduction in β-carotene after overexpressing carrot DcBCH1 in Arabidopsis [ 44 ]. There was no significant change in the amount of total carotenoid in transgenic kiwifruit leaves compared with the wild type, which may be attributed to the insignificant change in the transcription level of the PSY gene, as PSY has been reported to be closely related to the total amount of carotenoid flow [ 5 , 43 ].…”

Section: Discussionsupporting

confidence: 93%

Characterization and functional validation of β-carotene hydroxylase AcBCH genes in Actinidia chinensis

Xia

Zhou

Lin

et al. 2022

Horticulture Research

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Carotenoids are the pigment substance of yellow-fleshed kiwifruit, among which β-cryptoxanthin was only detected in the brighter yellow-fleshed variety “Jinshi 1”. β-carotene hydroxylase (BCH) catalyzes the formation of β-cryptoxanthin and zeaxanthin, but its molecular characteristics and functions have not been fully explained. Here, we isolated two β-carotene hydroxylase genes, AcBCH1 and AcBCH2 from kiwifruit (Actinidia chinensis), and their relative expression levels exhibited a close correlation with the content of β-cryptoxanthin. AcBCH1 catalyzed the formation of β-cryptoxanthin when transformed into the β-carotene-accumulating yeast cell. Moreover, silenced expression of AcBCH1 in kiwifruit caused a decrease in the contents of zeaxanthin, lutein and β-cryptoxanthin, and an increase in β-carotene content. The content of β-carotene decreased significantly after AcBCH1/2 were overexpressed in tomato. The content of zeaxanthin increased and β-carotene decreased in transgenic kiwifruit seedlings. The results will enrich our knowledge of the molecular mechanisms of carotenoids biosynthesis in kiwifruit.

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Section: Discussionsupporting

confidence: 93%

Characterization and functional validation of β-carotene hydroxylase AcBCH genes in Actinidia chinensis

Xia

Zhou

Lin

et al. 2022

Horticulture Research

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“…ABA is a hormone-reinforcing plant abiotic stress response [58] and can be produced in β-branches of carotenoids biosynthesis [59]. A previous study showed that abiotic stress can induce carotenoid synthesis to produce ABA in Arabidopsis to increase resistance [58,60,61]. This indicates that there is a potential relationship between carotenoids and ABA, and it can be reasonably inferred that the activation of carotenoid biosynthesis is related to ABA to boost plant salt tolerance and is worth further exploration.…”

Section: Discussionmentioning

confidence: 98%

Changes in Carotenoid Concentration and Expression of Carotenoid Biosynthesis Genes in Daucus carota Taproots in Response to Increased Salinity

Zhao

Deng

Wang³

et al. 2022

Horticulturae

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Studying the changes of carotenoids in the taproot of carrots under salt treatment is helpful to probe the salt stress response mechanism of carrots. The carotenoid concentration and the expression profiles of 10 carotenoid-related genes were determined in two carrot cultivars with different taproot colors. Under salt stress, the biosynthesis of carotenoids in the taproot of both ‘KRD’ and ‘BHJS’ was activated. RT-qPCR manifested that the expression levels of DcPSY1, DcPSY2, DcZDS1, DcCRT1 and DcCRT2 increased significantly in both ‘KRD’ and BHJS’ under salt stress, but DcCHXE transcripts decreased and DcPDS transcripts maintained a basal level compared to that of the control. In the taproot of ‘KRD’, the expression level of DcLCYB, DcLCYE and DcCHXB1 climbed dramatically. However, there was no significant change in the taproot of ‘BHJS’. The study showed that salt stress can stimulate the biosynthesis of carotenoids. The accumulation of lutein in the taproots of ‘KRD’ and ‘BHJS’ may be mainly attributed to the variation in DcLCYE and DcCHXB1 transcripts. The increase in β-carotene accumulation is speculated to increase salt tolerance.

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“…2) significantly reduced the seed germination percent of the majority of carrot accessions (Table 4). This reduction in the seed germination potential of sensitive carrot accessions under osmotic pressure might be in response to phytochrome-interacting factors that are directly associated with the regulation of abscisic acid (ABA) encoding genes (Li et al 2021). Recent studies also reported that phytochrome-interacting factors (DcPIF3) lower the drought stress tolerance of carrot by the overproduction of ABA (Wang et al 2022), and that a DREB-binding transcription factor from carrot enhances drought tolerance and regulates lignin biosynthesis in Arabidopsis (Li et al 2020).…”

Section: Resultsmentioning

confidence: 99%

Variation in Seed Germination and Amylase Activity of Diverse Carrot [Daucus carota (L.)] Germplasm under Simulated Drought Stress

Nijabat

Manzoor

Faiz

et al. 2023

horts

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Drought is one of the major environmental challenges constraining the production of agricultural crops, including carrot. Seed germination is the initial and most critical stage of crop establishment, and it is very sensitive to drought stress because water scarcity affects the enzymatic solubilization of stored metabolites in seeds that provide energy for the growth of germinating embryo. Few studies evaluating the effect of drought stress on carrot seed germination of more than a few cultivars grown under stress have been published. Therefore, the present study was designed to define the appropriate osmotic potential for evaluating drought tolerance of carrot, evaluate the response of diverse carrot germplasm to drought stress during seed germination to identify drought-tolerant accessions that may be used by plant breeders, and evaluate the relation between amylase activity and germination rate of drought-tolerant and drought-sensitive accessions. To identify an appropriate screening osmotic potential, two commercial cultivars and two United States Department of Agriculture inbreeds were evaluated at six osmotic potentials (00, −0.30, −0.51, −0.58, −0.80, and −1.05 MPa); −0.58 MPa was identified as the optimal osmotic potential for screening the drought tolerance of carrot seed. Cultivated and wild carrot plant introductions (PIs) (n = 200 and n = 50, respectively) from the National Plant Germplasm System were evaluated for drought tolerance under nonstress and simulated drought stress conditions (00 MPa and −0.58 MPa, respectively) by calculating the absolute decrease (AD) in percent germination, inhibition index (II), relative drought tolerance (RDT), and drought tolerance index (DTI). All measurements of drought tolerance identified significant differences among accessions; the AD in seed germination ranged from 0.0% to 69.3%, II ranged from 0.0% to 80.2%, RDT ranged from 0.2 to 1.0, and DTI ranged from 0.13 to 1.47. All wild carrot accessions displayed low levels of drought tolerance, but PI 652387 and PI 177381 (both from Turkey) and PI 274297 (Pakistan) were most drought-sensitive, whereas cultivated accessions PI 643114 (United States), PI 652208 (China), and PI 502347 (Uzbekistan) were most drought-tolerant. Tolerant accessions displayed much higher α-amylase activity under nonstress conditions than sensitive accessions, and α-amylase activity of tolerant accessions was also reduced less with seed germination under increasing osmotic potential (range, 0.0 to −1.05 MPa) than sensitive accessions over 24, 48, and 72 hours of seed germination. This is the first evaluation of drought stress tolerance during seed germination and the enzymatic response of diverse carrot germplasms under simulated drought stress.

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Overexpression of a carrot BCH gene, DcBCH1, improves tolerance to drought in Arabidopsis thaliana

Cited by 31 publications

References 71 publications

Characterization and functional validation of β-carotene hydroxylase AcBCH genes in Actinidia chinensis

Characterization and functional validation of β-carotene hydroxylase AcBCH genes in Actinidia chinensis

Changes in Carotenoid Concentration and Expression of Carotenoid Biosynthesis Genes in Daucus carota Taproots in Response to Increased Salinity

Variation in Seed Germination and Amylase Activity of Diverse Carrot [Daucus carota (L.)] Germplasm under Simulated Drought Stress

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