Overexpression of a Bcl-2-associated athanogene SlBAG9 negatively regulates high-temperature response in tomato

Ding, Haidong; Lü, Qian; Jiang, Hongrui; Ji, Yurong; Fang, Yifang; Sheng, Jiarong; Xu, Xiaoying; Ge, Cailin

doi:10.1016/j.ijbiomac.2021.11.114

Cited by 3 publications

(7 citation statements)

References 39 publications

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“…AtBAG7 played a key role in mediating the heat-induced UPR pathway [ 29 ]. Studies in the BAG family showed that BAG9 stimulated burning symptoms under heat and reduced the thermotolerance of tomato, which did not occur in our experiment [ 53 ]. By overexpressing BAG9 in Arabidopsis , the sensitivity to water scarcity, salinity, as well as ABA during the germination of seeds and the growth of seedlings were increased [ 54 ].…”

Section: Discussionmentioning

confidence: 52%

BAG9 Confers Thermotolerance by Regulating Cellular Redox Homeostasis and the Stability of Heat Shock Proteins in Solanum lycopersicum

et al. 2022

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The Bcl-2-associated athanogene (BAG) family, a group of co-chaperones that share conservative domains in flora and fauna, is involved in plant growth, development, and stress tolerance. However, the function of tomato BAG genes on thermotolerance remains largely unknown. Herein, we found that the expression of BAG9 was induced during heat stress in tomato plants. Knockout of the BAG9 gene by CRISPR/Cas9 reduced, while its overexpression increased thermotolerance in tomato plants as reflected by the phenotype, photosynthesis rate, and membrane peroxidation. Heat-induced reactive oxygen species and oxidative/oxidized proteins were further increased in bag9 mutants and were normalized in BAG9 overexpressing plants. Furthermore, the activities of antioxidant enzymes, ascorbic acid (AsA)/dehydroascorbic acid (DHA), and reduced glutathione (GSH)/oxidized glutathione (GSSG) were reduced in bag9 mutants and were increased in BAG9 overexpressing plants under heat stress. Additionally, BAG9 interacted with Hsp20 proteins in vitro and in vivo. Accumulation of Hsp proteins induced by heat showed a reduction in bag9 mutants; meanwhile, it was increased in BAG9 overexpressing plants. Thus, BAG9 played a crucial role in response to heat stress by regulating cellular redox homeostasis and the stability of heat shock proteins.

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Section: Discussionmentioning

confidence: 52%

BAG9 Confers Thermotolerance by Regulating Cellular Redox Homeostasis and the Stability of Heat Shock Proteins in Solanum lycopersicum

et al. 2022

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“…In our study, it has been found that HT induces AtBAG5 expression and the T-DNA mutant atbag5 exhibits increased basic thermotolerance (data not shown). Interestingly, in our previous study on the SlBAG9 function, a homologous gene of AtBAG5 in tomato, further proved that it negatively regulates heat tolerance (Ding et al, 2020;Ding et al, 2022;Jiang et al, 2022). HT (high temperature) induces high expression levels of SlBAG9 at the transcriptional and protein levels, and SlBAG9-overexpressing plants of Arabidopsis and tomato show high sensitivity to HT stress (Ding et al, 2020;Ding et al, 2022).…”

Section: Heat Stressmentioning

confidence: 94%

“…Interestingly, in our previous study on the SlBAG9 function, a homologous gene of AtBAG5 in tomato, further proved that it negatively regulates heat tolerance (Ding et al, 2020;Ding et al, 2022;Jiang et al, 2022). HT (high temperature) induces high expression levels of SlBAG9 at the transcriptional and protein levels, and SlBAG9-overexpressing plants of Arabidopsis and tomato show high sensitivity to HT stress (Ding et al, 2020;Ding et al, 2022). SlBAG9 promoter can drive GUS gene expression in tobacco leaves under normal or HT condition, and the HSE1 is essential for HTinduced GUS activity (Ding et al, 2022).…”

Section: Heat Stressmentioning

confidence: 95%

“…Recently, there have been more studies on the function of BAGs in a variety of plants (summarized in Table 1 ). For example, the involvement of tomato BAGs in fruit ripening, environmental stress, and dark-induced leaf senescence was reported ( Ding et al., 2020 ; He et al., 2021 ; Irfan et al., 2021 ; Ding et al., 2022 ; Jiang et al., 2022 ). The evolution of rice BAGs and their roles in cell death were studied ( Bansal et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

“…He et al (2021);Ding et al (2022) Dark-induced leaf senescence; Heat; DroughtDing et al (2020, Ding et al, 2022;He et al (2021);Jiang et al (2022) …”

mentioning

confidence: 99%

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Functional insights of plant bcl-2–associated ahanogene (BAG) proteins: Multi-taskers in diverse cellular signal transduction pathways

et al. 2023

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Bcl-2-associated athanogene (BAG) gene family is a highly conserved molecular chaperone cofactor in evolution from yeast to humans and plants playing important roles in a variety of signal pathways. Plant BAG proteins have special structures, especially those containing CaM-binding IQ motifs which are unique to plants. While early studies focused more on the structure and physiological function of plant BAGs, recent studies have revealed many novel functional mechanisms involved in multiple cellular processes. How to achieve signal specificity has become an interesting topic of plant BAG research. In this review, we have provided a historic view of plant BAG research and summarized recent advances in the establishment of BAG as essential components in normal plant growth, environmental stress response, and plant immunity. Based on the relationship between BAG proteins and their newly interacting proteins, this review highlights the functional mechanisms of various cellular signals mediated by plant BAGs. Future work needs to focus on the post-translational modification of BAG proteins, and on understanding how specificity is achieved among BAG signaling pathways.

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Genome-Wide Identification of the Bcl-2 Associated Athanogene (BAG) Gene Family in Solanum lycopersicum and the Functional Role of SlBAG9 in Response to Osmotic Stress

Jiang

Sheng

et al. 2022

Antioxidants

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The Bcl-2-associated athanogene (BAG) proteins are a family of multi-functional group of co-chaperones regulators, modulating diverse processes from plant growth and development to stress response. Here, 10 members of SlBAG gene family were identified based on the available tomato (Solanum lycopersicum) genomic information and named as SlBAG1-10 according to their chromosomal location. All SlBAG proteins harbor a characteristic BAG domain, categorized into two groups, and SlBAG4, SlBAG7, and SlBAG9 of group I contain a plant-specific isoleucine glutamine (IQ) calmodulin-binding motif located in the N terminus. The quantitative real-time PCR expression analysis revealed that these SlBAG genes had organ-specific expression patterns and most SlBAG genes were differentially expressed in multiple abiotic stresses including drought, salt, high temperature, cold, and cadmium stress as well as abscisic acid and H2O2. In addition, heterologous overexpression of SlBAG9 increased the sensitivity of Arabidopsis to drought, salt, and ABA during seed germination and seedling growth. The decreased tolerance may be due to the downregulation of stress-related genes expression and severe oxidative stress. The expression levels of some stress and ABA-related genes, such as ABI3, RD29A, DREB2A, and P5CS1, were significantly inhibited by SlBAG9 overexpression under osmotic stress. Meanwhile, the overexpression of SlBAG9 inhibited the expression of FSD1 and CAT1 under stress conditions and the decreased levels of superoxide dismutase and catalase enzyme activities were detected accompanying the trends in the expression of both genes, which resulted in H2O2 accumulation and lipid peroxidation. Taken together, these findings lay a foundation for the future study of the biological function of SlBAG genes in tomato.

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Overexpression of a Bcl-2-associated athanogene SlBAG9 negatively regulates high-temperature response in tomato

Cited by 3 publications

References 39 publications

BAG9 Confers Thermotolerance by Regulating Cellular Redox Homeostasis and the Stability of Heat Shock Proteins in Solanum lycopersicum

BAG9 Confers Thermotolerance by Regulating Cellular Redox Homeostasis and the Stability of Heat Shock Proteins in Solanum lycopersicum

Functional insights of plant bcl-2–associated ahanogene (BAG) proteins: Multi-taskers in diverse cellular signal transduction pathways

Genome-Wide Identification of the Bcl-2 Associated Athanogene (BAG) Gene Family in Solanum lycopersicum and the Functional Role of SlBAG9 in Response to Osmotic Stress

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